##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781144_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	61030
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.351663116500085	34.0	34.0	34.0	31.0	34.0
2	32.50101589382271	34.0	34.0	34.0	31.0	34.0
3	32.51120760281829	34.0	34.0	34.0	31.0	34.0
4	32.56418155005735	34.0	34.0	34.0	31.0	34.0
5	32.61443552351302	34.0	34.0	34.0	31.0	34.0
6	35.90188431918728	38.0	37.0	38.0	32.0	38.0
7	35.83680157299689	38.0	37.0	38.0	32.0	38.0
8	35.63357365230215	38.0	37.0	38.0	31.0	38.0
9	35.91815500573488	38.0	37.0	38.0	32.0	38.0
10-11	35.81872849418319	38.0	37.0	38.0	31.5	38.0
12-13	35.78675241684418	38.0	37.0	38.0	31.0	38.0
14-15	35.75782402097329	38.0	37.0	38.0	31.0	38.0
16-17	35.72258725217107	38.0	37.0	38.0	31.0	38.0
18-19	35.747067016221536	38.0	37.0	38.0	31.0	38.0
20-21	35.707348844830406	38.0	37.0	38.0	31.0	38.0
22-23	35.733598230378504	38.0	37.0	38.0	31.5	38.0
24-25	35.72970670162215	38.0	37.0	38.0	31.0	38.0
26-27	35.70208913649025	38.0	37.0	38.0	31.0	38.0
28-29	35.76387842044896	38.0	37.0	38.0	31.0	38.0
30-31	35.81837620842209	38.0	37.0	38.0	31.5	38.0
32-33	35.807537276749144	38.0	37.0	38.0	31.5	38.0
34-35	35.82203014910699	38.0	37.0	38.0	31.5	38.0
36-37	35.72628215631657	38.0	37.0	38.0	31.0	38.0
38-39	35.73975094215959	38.0	37.0	38.0	31.0	38.0
40-41	35.706291987547104	38.0	37.0	38.0	31.0	38.0
42-43	35.761264951663115	38.0	37.0	38.0	31.0	38.0
44-45	35.7762084220875	38.0	37.0	38.0	31.5	38.0
46-47	35.75580042601999	38.0	37.0	38.0	31.5	38.0
48-49	35.721669670653775	38.0	37.0	38.0	31.0	38.0
50-51	35.62779780435851	38.0	37.0	38.0	31.0	38.0
52-53	35.68524496149435	38.0	37.0	38.0	31.0	38.0
54-55	35.65155661150254	38.0	37.0	38.0	31.0	38.0
56-57	35.59671473046043	38.0	37.0	38.0	31.0	38.0
58-59	35.61440275274455	38.0	37.0	38.0	31.0	38.0
60-61	35.59672292315255	38.0	37.0	38.0	31.0	38.0
62-63	35.57901851548419	38.0	37.0	38.0	31.0	38.0
64-65	35.596444371620514	38.0	37.0	38.0	31.0	38.0
66-67	35.53077175159758	38.0	37.0	38.0	31.0	38.0
68-69	35.23211535310503	38.0	36.0	38.0	28.5	38.0
70-71	35.436432901851546	38.0	36.5	38.0	31.0	38.0
72-73	35.480149106996556	38.0	37.0	38.0	31.0	38.0
74-75	35.45218744879568	38.0	37.0	38.0	31.0	38.0
76	34.26350974930362	37.0	34.0	38.0	25.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	11.0
14	33.0
15	113.0
16	199.0
17	275.0
18	328.0
19	280.0
20	263.0
21	220.0
22	229.0
23	260.0
24	263.0
25	316.0
26	336.0
27	458.0
28	537.0
29	728.0
30	938.0
31	1207.0
32	1570.0
33	2170.0
34	3236.0
35	5236.0
36	10194.0
37	31629.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.39996722923152	20.052433229559234	10.340815992135017	26.206783549074224
2	22.184171718826807	20.208094379813208	31.9760773390136	25.63165656234639
3	19.336391938390957	21.63362280845486	28.905456332951008	30.12452892020318
4	14.102900213009995	16.409962313616255	35.96100278551532	33.52613468785843
5	14.281500901196134	33.986563984925446	35.20399803375389	16.52793708012453
6	30.5849582172702	36.33786662297231	17.794527281664756	15.282647878092742
7	29.69031623791578	28.356545961002787	22.159593642470917	19.79354415861052
8	25.965918400786496	34.09962313616254	19.601835163034572	20.332623300016387
9	24.635425200720956	16.513190234310997	21.425528428641652	37.425856136326395
10-11	25.479272488939863	24.573160740619368	26.6172374242176	23.33032934622317
12-13	24.94265115516959	22.910863509749305	26.51155169588727	25.63493363919384
14-15	23.08700639029985	24.03653940684909	23.628543339341306	29.247910863509752
16-17	23.866131410781584	26.644273308209076	25.05407176798296	24.435523513026382
18-19	23.032934622316894	26.382926429624774	26.310011469768966	24.274127478289365
20-21	22.76011797476651	26.42798623627724	26.56726200229396	24.2446337866623
22-23	23.402425036867115	26.04047189906603	26.43535965918401	24.121743404882846
24-25	22.871538587579877	26.24201212518433	26.395215467802718	24.491233819433067
26-27	22.267737178436832	26.699164345403897	26.73766999836146	24.295428477797802
28-29	23.061609044732098	26.282975585777486	27.357037522529904	23.29837784696051
30-31	22.979682123545796	26.220711125675898	26.88104211043749	23.91856464034082
32-33	22.598721940029492	26.759790267081762	26.640176962149763	24.00131083073898
34-35	23.326233000163853	25.933147632311975	26.97116172374242	23.769457643781745
36-37	23.445846305095856	25.86269048009176	26.349336391938387	24.342126822873997
38-39	22.216123218089464	26.44846796657382	27.523349172538097	23.812059642798626
40-41	22.62248074717352	25.870063902998524	27.48975913485171	24.01769621497624
42-43	22.57496313288547	25.802064558413896	27.138292642962476	24.484679665738163
44-45	22.111256758971	26.225626740947078	27.243159102080945	24.41995739800098
46-47	22.52744551859741	25.636572177617566	27.393085367851878	24.44289693593315
48-49	21.96542683925938	25.75290840570211	27.788792397181716	24.492872357856793
50-51	22.04981156808127	26.05276093724398	27.52171063411437	24.37571686056038
52-53	21.79092249713256	25.59397017860069	28.037850237588067	24.577257086678685
54-55	21.545141733573654	25.87088317221039	27.92888743241029	24.65508766180567
56-57	21.069146321481238	26.019170899557594	27.883008356545965	25.028674422415204
58-59	21.37964935277732	25.893822710142555	27.672456169097163	25.05407176798296
60-61	20.90119613304932	25.616909716532852	28.22464361789284	25.25725053252499
62-63	20.66442733082091	25.494838603965263	28.39423234474848	25.44650172046535
64-65	21.489431427166966	25.807799442896933	27.663444207766673	25.039324922169428
66-67	21.18384401114206	25.515320334261837	27.45125348189415	25.84958217270195
68-69	20.983123054235623	25.638210716041293	27.158774373259053	26.219891856464034
70-71	21.709814845158117	25.818449942651156	26.99983614615763	25.4718990660331
72-73	21.89251187940357	25.334261838440113	26.619695231853186	26.15353105030313
74-75	22.083401605767655	25.486645911846633	26.586105194166805	25.843847288218907
76	22.428313943961985	25.605439947566772	26.65082746190398	25.315418646567263
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	2.5
12	4.0
13	3.5
14	6.0
15	6.5
16	12.5
17	21.0
18	27.5
19	39.5
20	59.0
21	73.0
22	81.5
23	132.0
24	210.5
25	247.0
26	296.0
27	398.0
28	512.0
29	573.0
30	640.5
31	766.0
32	1003.0
33	1182.0
34	1279.0
35	1534.0
36	1926.5
37	2161.0
38	2305.5
39	2666.5
40	3160.0
41	3530.5
42	3624.0
43	3672.0
44	3672.0
45	3586.0
46	3548.0
47	3506.0
48	3264.0
49	2942.5
50	2821.0
51	2596.5
52	2100.5
53	1679.5
54	1530.0
55	1404.5
56	1176.0
57	985.5
58	898.0
59	819.0
60	672.5
61	603.5
62	602.0
63	568.5
64	563.0
65	595.5
66	619.0
67	638.0
68	628.0
69	586.0
70	584.0
71	614.0
72	634.5
73	616.0
74	519.0
75	461.0
76	429.5
77	357.5
78	298.5
79	280.0
80	248.0
81	183.0
82	124.0
83	98.0
84	75.0
85	48.0
86	33.0
87	22.0
88	14.5
89	8.0
90	7.5
91	3.5
92	1.0
93	2.0
94	2.0
95	0.5
96	0.0
97	0.5
98	0.5
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	61030.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.56808127150582
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.49833666001331	45.251515648041945
2	14.536260811709914	17.89939374078322
3	4.9634065202927475	9.167622480747173
4	2.421823020625416	5.964279862362773
5	1.5196274118429807	4.6780271997378335
6	0.7984031936127743	2.9493691627068657
7	0.5748502994011976	2.477470096673767
8	0.45242847638057215	2.2284122562674096
9	0.26879574184963406	1.489431427166967
>10	0.9660678642714572	7.894478125512043
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0016385384237260364	0.0
5	0.0	0.0	0.0	0.0016385384237260364	0.0
6	0.0	0.0	0.0	0.0016385384237260364	0.0
7	0.0	0.0	0.0	0.0016385384237260364	0.0
8	0.0	0.0	0.0	0.0016385384237260364	0.0
9	0.0	0.0	0.0	0.0016385384237260364	0.0
10	0.0	0.0	0.0	0.0016385384237260364	0.0
11	0.0	0.0	0.0	0.0016385384237260364	0.0
12	0.0	0.0	0.0	0.0016385384237260364	0.0
13	0.0	0.0	0.0	0.0016385384237260364	0.0
14	0.0	0.0	0.0	0.0016385384237260364	0.0
15	0.0	0.0	0.0	0.0016385384237260364	0.0
16	0.0	0.0	0.0	0.0016385384237260364	0.0
17	0.0	0.0	0.0	0.0016385384237260364	0.0
18	0.0	0.0	0.0	0.0016385384237260364	0.0
19	0.0	0.0	0.0	0.0016385384237260364	0.0
20	0.0	0.0	0.0	0.0016385384237260364	0.0
21	0.0	0.0	0.0	0.0016385384237260364	0.0
22	0.0	0.0	0.0	0.004915615271178109	0.0
23	0.0	0.0	0.0	0.006554153694904146	0.0
24	0.0	0.0	0.0	0.013108307389808291	0.0
25	0.0	0.0	0.0	0.014746845813534328	0.0
26	0.0	0.0	0.0	0.016385384237260363	0.0
27	0.0	0.0	0.0	0.019662461084712438	0.0
28	0.0	0.0	0.0	0.03440930689824676	0.0
29	0.0	0.0	0.0	0.045879075864329016	0.0
30	0.0	0.0	0.0	0.052433229559233165	0.0
31	0.0	0.0	0.0	0.06062592167786335	0.0
32	0.0	0.0	0.0	0.07537276749139768	0.0
33	0.0	0.0	0.0	0.09667376699983615	0.0
34	0.0	0.0	0.0	0.1212518433557267	0.0
35	0.0	0.0	0.0	0.18679338030476814	0.0
36	0.0	0.0	0.0	0.2736359167622481	0.0
37	0.0	0.0	0.0	0.4178272980501393	0.0
38	0.0	0.0	0.0	0.5620186793380305	0.0
39	0.0	0.0	0.0	0.7488120596427986	0.0
40	0.0	0.0	0.0	0.9749303621169917	0.0
41	0.0	0.0	0.0	1.2534818941504178	0.0
42	0.0	0.0	0.0	1.5861051941668032	0.0
43	0.0	0.0	0.0	1.9990168769457644	0.0
44	0.0	0.0	0.0	2.482385711944945	0.0
45	0.0	0.0	0.0	2.9919711617237423	0.0
46	0.0	0.0	0.0	3.506472226773718	0.0
47	0.0	0.0	0.0	4.078322136654105	0.0
48	0.0	0.0	0.0	4.630509585449778	0.0
49	0.0	0.0	0.0	5.145010650499755	0.0
50	0.0	0.0	0.0	5.6922824840242505	0.0
51	0.0	0.0	0.0	6.214976241192856	0.0
52	0.0	0.0	0.0	6.806488612157955	0.0
53	0.0	0.0	0.0	7.309519908241848	0.0
54	0.0	0.0	0.0	7.8780927412747825	0.0
55	0.0	0.0	0.0	8.526953957070294	0.0
56	0.0	0.0	0.0	9.16106832705227	0.0
57	0.0	0.0	0.0	9.74766508274619	0.0
58	0.0	0.0	0.0	10.294936916270686	0.0
59	0.0	0.0	0.0	10.848762903490087	0.0
60	0.0	0.0	0.0	11.407504505980665	0.0
61	0.0	0.0	0.0	11.856464034081599	0.0
62	0.0	0.0	0.0	12.390627560216288	0.0
63	0.0	0.0	0.0	12.928068163198427	0.0
64	0.0	0.0	0.0	13.458954612485662	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTGCCG	15	0.0022147913	70.0	64
GCTTGTA	15	0.0022147913	70.0	1
CCAGTAA	15	0.0022147913	70.0	26
CGTTACT	25	2.3730705E-4	56.000004	17
CGACGTT	20	0.0069206776	52.500004	14
CCGATGC	20	0.0069206776	52.500004	33
ACGTTAC	20	0.0069206776	52.500004	16
GACGTTA	20	0.0069206776	52.500004	15
GACCGGG	20	0.0069206776	52.500004	32
CCGGAAT	20	0.0069206776	52.500004	3
AAGGTGA	20	0.0069206776	52.500004	53
TTCCGGC	20	0.0069206776	52.500004	54
TCGGCCA	20	0.0069206776	52.500004	21
ATCCGGA	20	0.0069206776	52.500004	1
CAAGGGT	20	0.0069206776	52.500004	32
CGCTACA	20	0.0069206776	52.500004	24
GGTATAC	20	0.0069206776	52.500004	39
ACTCGGA	20	0.0069206776	52.500004	21
TACTCGG	20	0.0069206776	52.500004	20
TACACGA	20	0.0069206776	52.500004	27
>>END_MODULE