FastQCFastQC Report
Wed 25 May 2016
SRR1781144_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781144_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58131
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT5700.9805439438509573No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCTT150.002214258570.0000153
TTGGGAT150.002214258570.0000110
CGGACCT150.002214258570.0000121
TCGTATG1350.057.03703740
CCGTCTT1300.056.5384647
GCCGTCT1300.056.5384646
CGTATGC1400.055.041
TATGCCG1400.055.043
TCTCGTA1150.054.78260838
CGTCTTC1350.054.44444348
CTCGTAT1300.053.84615339
GTATGCC1450.053.10344742
GTATCTC1200.052.50000435
CGGCATG200.006919028752.519
CTGAGGA200.006919028752.518
GGTCACT200.006919028752.570
GTAAGGA200.006919028752.53
CCTGCCA200.006919028752.58
ATACATC200.006919028752.537
GTCTTCT1300.051.15384749
Produced by FastQC (version 0.11.5)