##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781144_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	58131
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.869191997385215	34.0	34.0	34.0	31.0	34.0
2	32.94404018509917	34.0	34.0	34.0	31.0	34.0
3	33.04973250073111	34.0	34.0	34.0	31.0	34.0
4	33.1756721886773	34.0	34.0	34.0	32.0	34.0
5	33.12855447179646	34.0	34.0	34.0	31.0	34.0
6	36.18626894428102	38.0	36.0	38.0	34.0	38.0
7	36.05547814419157	38.0	36.0	38.0	32.0	38.0
8	35.712631814350345	38.0	36.0	38.0	31.0	38.0
9	35.746193941270576	38.0	36.0	38.0	31.0	38.0
10-11	35.66169513684609	38.0	36.0	38.0	31.0	38.0
12-13	35.64131014432919	38.0	36.0	38.0	31.0	38.0
14-15	35.639804923362746	38.0	36.0	38.0	31.0	38.0
16-17	35.566479159140556	38.0	36.0	38.0	31.0	38.0
18-19	35.60126266535927	38.0	36.0	38.0	31.0	38.0
20-21	35.494262957802206	38.0	36.0	38.0	31.0	38.0
22-23	35.5778672309095	38.0	36.0	38.0	31.0	38.0
24-25	35.54727253960881	38.0	36.0	38.0	31.0	38.0
26-27	35.27140424214275	37.5	35.5	38.0	30.5	38.0
28-29	35.228165694723984	37.0	35.0	38.0	30.0	38.0
30-31	35.28674889473775	37.5	35.5	38.0	31.0	38.0
32-33	35.15517537974574	37.0	35.0	38.0	27.0	38.0
34-35	35.20644750649396	37.0	35.0	38.0	28.5	38.0
36-37	35.15039307770381	37.0	35.0	38.0	28.0	38.0
38-39	35.09737489463453	37.0	35.0	38.0	27.0	38.0
40-41	35.141034903923895	37.0	35.0	38.0	27.0	38.0
42-43	35.11812974144605	37.0	35.0	38.0	27.0	38.0
44-45	35.069231563193476	37.0	35.0	38.0	27.0	38.0
46-47	35.117562058110124	37.0	35.0	38.0	27.0	38.0
48-49	35.07417728922606	37.0	35.0	38.0	27.0	38.0
50-51	34.959049388450225	37.0	35.0	38.0	27.0	38.0
52-53	35.01164610964889	37.0	35.0	38.0	27.0	38.0
54-55	35.01084619222102	37.0	35.0	38.0	27.0	38.0
56-57	34.948874094717105	37.0	35.0	38.0	27.0	38.0
58-59	34.929615867609364	37.0	35.0	38.0	27.0	38.0
60-61	34.93811391512274	37.0	35.0	38.0	27.0	38.0
62-63	34.8738624830125	37.0	35.0	38.0	27.0	38.0
64-65	34.83784899623265	37.0	35.0	38.0	27.0	38.0
66-67	34.812793518088455	37.0	35.0	38.0	27.0	38.0
68-69	34.721026646711735	37.0	34.0	38.0	27.0	38.0
70-71	34.66440453458568	37.0	34.0	38.0	27.0	38.0
72-73	34.603576405016256	37.0	34.0	38.0	26.5	38.0
74-75	34.563055856599746	37.0	34.0	38.0	26.0	38.0
76	33.527721869570456	37.0	34.0	38.0	23.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	2.0
21	5.0
22	18.0
23	27.0
24	82.0
25	155.0
26	322.0
27	580.0
28	802.0
29	1309.0
30	1985.0
31	2682.0
32	3705.0
33	4787.0
34	6274.0
35	7949.0
36	11092.0
37	16355.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.69838812337651	20.09771034387848	10.713732775971513	26.490168756773496
2	22.623041062427966	21.51519843112969	31.73177822504344	24.12998228139891
3	21.24511878343741	23.05654470076207	28.721336292167692	26.977000223632828
4	15.035007139048012	17.997282000997746	35.64191223271576	31.325798627238477
5	14.78385026921952	34.405050661437095	34.94176945175552	15.869329617587862
6	31.20366069739038	37.26583062393559	17.4622834632124	14.06822521546163
7	29.698439730952504	29.12731588997265	21.639056613510864	19.535187765563986
8	25.66788804596515	35.67459703084413	18.924498116323477	19.733016806867248
9	24.969465517537976	16.801706490512807	21.109218833324732	37.119609158624485
10-11	26.212347972682387	25.396088145739792	26.359429564260033	22.032134317317784
12-13	25.37630524160947	22.762381517606784	26.54607696409833	25.315236276685415
14-15	23.473705940031998	23.948495639159827	23.377371798179972	29.2004266226282
16-17	24.806901653162683	27.551564569678828	24.506717586141647	23.134816191016842
18-19	24.18675061499028	26.46780547384356	25.410710292270906	23.934733618895255
20-21	23.035041544098675	26.686277545543685	25.706593727959266	24.572087182398374
22-23	23.651752077204936	26.74218575286852	25.88119936006606	23.724862809860486
24-25	24.13428291273159	26.718102217405516	25.770243071682923	23.377371798179972
26-27	23.785931774784537	26.90044898591113	26.07988852763586	23.233730711668475
28-29	23.948495639159827	26.036022088042525	26.84024014725362	23.17524212554403
30-31	23.55541793535291	26.81787686432368	26.10139168429926	23.525313516024152
32-33	22.926665634515146	26.50135039823846	27.401902599301575	23.170081367944814
34-35	24.487794808277854	26.646711737283034	26.295780220536376	22.569713233902736
36-37	23.72142230479434	26.312982745867092	26.798954086459894	23.16664086287867
38-39	22.950749169978153	26.620907949286952	27.026027420825375	23.402315459909513
40-41	22.88193906865528	25.644664636768677	27.415664619566154	24.05773167500989
42-43	23.650891950938398	25.73497789475495	27.25998176532315	23.354148388983504
44-45	22.645404345357896	26.54521683783179	27.514579140217784	23.294799676592522
46-47	23.71196091586245	25.31093564535274	27.01828628442655	23.958817154358258
48-49	23.18556364074246	25.311795771619273	27.23933873492629	24.263301852711976
50-51	22.479399975916465	25.620581101305675	27.75541449484784	24.14460442793002
52-53	22.330598131805747	25.322977413084242	28.967332404396966	23.379092050713044
54-55	21.811941993084584	25.475219762261098	28.29213328516626	24.420704959488052
56-57	22.14051022690131	25.634343121570247	27.467272195558305	24.757874455970136
58-59	21.75775403829282	25.433073575200837	28.663707832309782	24.145464554196554
60-61	22.19297792916	25.986994890849978	27.294386815984588	24.525640364005437
62-63	20.762587947910756	26.874645197915054	27.417384872099227	24.94538198207497
64-65	21.189210576112576	27.200633052932172	26.67079527274604	24.93936109820922
66-67	21.339732672756362	26.792073076327604	26.245032770810756	25.623161480105278
68-69	21.034387848136106	27.31416972011491	26.190844816018995	25.46059761572999
70-71	21.548743355524593	26.697459187008654	26.117734083363437	25.63606337410332
72-73	21.697545199635307	25.99645627978187	26.202026457483957	26.103972063098862
74-75	21.62701484577936	26.09107016910082	25.91388415819442	26.36803082692539
76	22.239424747552942	24.971185770071045	26.40759663518605	26.381792847189967
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.5
14	4.0
15	6.0
16	13.0
17	19.0
18	16.0
19	30.5
20	53.5
21	59.0
22	76.0
23	122.5
24	199.0
25	246.0
26	286.0
27	368.5
28	468.5
29	526.0
30	610.0
31	739.5
32	946.0
33	1107.0
34	1218.0
35	1482.5
36	1763.5
37	1891.0
38	2054.5
39	2484.0
40	3135.0
41	3509.0
42	3498.0
43	3559.0
44	3629.5
45	3559.0
46	3479.0
47	3423.5
48	3113.0
49	2722.0
50	2586.0
51	2363.0
52	1926.5
53	1567.5
54	1422.0
55	1328.5
56	1134.5
57	911.5
58	789.0
59	727.5
60	613.5
61	571.0
62	581.0
63	565.0
64	540.5
65	559.5
66	576.0
67	565.0
68	589.5
69	608.5
70	600.0
71	597.0
72	596.0
73	572.0
74	492.0
75	435.0
76	428.5
77	350.0
78	255.0
79	232.0
80	207.5
81	157.5
82	115.0
83	98.0
84	76.5
85	45.5
86	29.5
87	23.0
88	16.5
89	7.5
90	3.5
91	2.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	58131.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.11831896922468
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.08197175297701	46.01847551220519
2	14.353364718914428	17.83213775782285
3	4.976460814178898	9.27388140579037
4	2.256992522846857	5.6080232578142475
5	1.3569648296870673	4.2146187060260445
6	0.949875380780947	3.540279713061877
7	0.5926336194959845	2.576938294541639
8	0.3849349210744946	1.9129208167759026
9	0.2353918582110219	1.315993187799969
>10	0.8031016338964275	6.5352393731399765
>50	0.005538631957906397	0.1909480311709759
>100	0.0	0.0
>500	0.0027693159789531985	0.9805439438509573
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT	570	0.9805439438509573	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.001720252533071855	0.0
21	0.0	0.0	0.0	0.00344050506614371	0.0
22	0.0	0.0	0.0	0.00344050506614371	0.0
23	0.0	0.0	0.0	0.00344050506614371	0.0
24	0.0	0.0	0.0	0.00344050506614371	0.0
25	0.0	0.0	0.0	0.0051607575992155645	0.0
26	0.0	0.0	0.0	0.00688101013228742	0.0
27	0.0	0.0	0.0	0.010321515198431129	0.0
28	0.0	0.0	0.0	0.029244293062221535	0.0
29	0.0	0.0	0.0	0.04472656585986823	0.0
30	0.0	0.0	0.0	0.051607575992155645	0.0
31	0.0	0.0	0.0	0.05676833359137121	0.0
32	0.0	0.0	0.0	0.07225060638901791	0.0
33	0.0	0.0	0.0	0.09633414185202388	0.0
34	0.0	0.0	0.0	0.1221379298481017	0.0
35	0.0	0.0	0.0	0.18578727357176034	0.0
36	0.0	0.0	0.0	0.2717999002253531	0.0
37	0.0	0.0	0.0	0.42490237566874817	0.0
38	0.0	0.0	0.0	0.569403588446784	0.0
39	0.0	0.0	0.0	0.7448693468201132	0.0
40	0.0	0.0	0.0	0.9685021761194543	0.0
41	0.0	0.0	0.0	1.2523438440763104	0.0
42	0.0	0.0	0.0	1.5619893000292442	0.0
43	0.0	0.0	0.0	1.967968897834202	0.0
44	0.0	0.0	0.0	2.4513598596273933	0.0
45	0.0	0.0	0.0	2.9571141043505187	0.0
46	0.0	0.0	0.0	3.4559873389413567	0.0
47	0.0	0.0	0.0	4.009908654590494	0.0
48	0.0	0.0	0.0	4.548347697441985	0.0
49	0.0	0.0	0.0	5.048941184565894	0.0
50	0.0	0.0	0.0	5.5925409850166	0.0
51	0.0	0.0	0.0	6.1103369974712285	0.0
52	0.0	0.0	0.0	6.688341848583372	0.0
53	0.0	0.0	0.0	7.185494830641138	0.0
54	0.0	0.0	0.0	7.7290946310918445	0.0
55	0.0	0.0	0.0	8.36558806832843	0.0
56	0.0	0.0	0.0	9.00552201063116	0.0
57	0.0	0.0	0.0	9.621372417470885	0.0
58	0.0	0.0	0.0	10.166692470454663	0.0
59	0.0	0.0	0.0	10.74813782663295	0.0
60	0.0	0.0	0.0	11.284856616951368	0.0
61	0.0	0.0	0.0	11.737283033149266	0.0
62	0.0	0.0	0.0	12.246477782938536	0.0
63	0.0	0.0	0.0	12.76943455299238	0.0
64	0.0	0.0	0.0	13.297552080645438	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGCTT	15	0.0022142585	70.00001	53
TTGGGAT	15	0.0022142585	70.00001	10
CGGACCT	15	0.0022142585	70.00001	21
TCGTATG	135	0.0	57.037037	40
CCGTCTT	130	0.0	56.53846	47
GCCGTCT	130	0.0	56.53846	46
CGTATGC	140	0.0	55.0	41
TATGCCG	140	0.0	55.0	43
TCTCGTA	115	0.0	54.782608	38
CGTCTTC	135	0.0	54.444443	48
CTCGTAT	130	0.0	53.846153	39
GTATGCC	145	0.0	53.103447	42
GTATCTC	120	0.0	52.500004	35
CGGCATG	20	0.0069190287	52.5	19
CTGAGGA	20	0.0069190287	52.5	18
GGTCACT	20	0.0069190287	52.5	70
GTAAGGA	20	0.0069190287	52.5	3
CCTGCCA	20	0.0069190287	52.5	8
ATACATC	20	0.0069190287	52.5	37
GTCTTCT	130	0.0	51.153847	49
>>END_MODULE