FastQCFastQC Report
Wed 25 May 2016
SRR1781143_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781143_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71487
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGTAC150.002216351270.040
TGCGGCA150.002216351270.035
TTACGTT150.002216351270.052
TATGTTC150.002216351270.028
TTTTACG150.002216351270.050
GGAATGG200.00692551752.510
TGCTTAC200.00692551752.57
TCCGACA200.00692551752.566
CTGAGTC200.00692551752.540
TTACTCG200.00692551752.519
CGCCTAG200.00692551752.516
TACTGTA200.00692551752.550
TAATTCA200.00692551752.528
GCTTACC200.00692551752.58
GTATCCG200.00692551752.563
TATAGTC200.00692551752.55
CAAGAGT200.00692551752.54
GGTCTAC200.00692551752.51
ACAAGCA200.00692551752.534
TACGTTG200.00692551752.553
Produced by FastQC (version 0.11.5)