##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781143_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	71487
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.60943947850658	34.0	34.0	34.0	31.0	34.0
2	32.881125239554045	34.0	34.0	34.0	32.0	34.0
3	32.84316029487879	34.0	34.0	34.0	32.0	34.0
4	32.95242491641837	34.0	34.0	34.0	32.0	34.0
5	32.959908794606015	34.0	34.0	34.0	33.0	34.0
6	36.27501503769916	38.0	37.0	38.0	34.0	38.0
7	36.28973100004197	38.0	37.0	38.0	34.0	38.0
8	36.16086840964091	38.0	37.0	38.0	34.0	38.0
9	36.30057213199603	38.0	37.0	38.0	34.0	38.0
10-11	36.25876033404675	38.0	37.0	38.0	34.0	38.0
12-13	36.251157553121544	38.0	37.0	38.0	34.0	38.0
14-15	36.196343391106076	38.0	37.0	38.0	34.0	38.0
16-17	36.21201057534937	38.0	37.0	38.0	34.0	38.0
18-19	36.20635919817589	38.0	37.0	38.0	34.0	38.0
20-21	36.175164715262916	38.0	37.0	38.0	34.0	38.0
22-23	36.205072250898766	38.0	37.0	38.0	34.0	38.0
24-25	36.200903660805466	38.0	37.0	38.0	34.0	38.0
26-27	36.1617147173612	38.0	37.0	38.0	34.0	38.0
28-29	36.22743295983885	38.0	37.0	38.0	34.0	38.0
30-31	36.274539426748916	38.0	37.0	38.0	34.0	38.0
32-33	36.25074489067943	38.0	37.0	38.0	34.0	38.0
34-35	36.26592247541511	38.0	37.0	38.0	34.0	38.0
36-37	36.197189698826364	38.0	37.0	38.0	34.0	38.0
38-39	36.178361100619696	38.0	37.0	38.0	34.0	38.0
40-41	36.16699539776463	38.0	37.0	38.0	34.0	38.0
42-43	36.230741253654514	38.0	37.0	38.0	34.0	38.0
44-45	36.22475415110439	38.0	37.0	38.0	34.0	38.0
46-47	36.216284079622866	38.0	37.0	38.0	34.0	38.0
48-49	36.189328129589995	38.0	37.0	38.0	34.0	38.0
50-51	36.1061032075762	38.0	37.0	38.0	34.0	38.0
52-53	36.15018115181782	38.0	37.0	38.0	34.0	38.0
54-55	36.10236826276106	38.0	37.0	38.0	34.0	38.0
56-57	36.073041252255656	38.0	37.0	38.0	34.0	38.0
58-59	36.08267936827674	38.0	37.0	38.0	34.0	38.0
60-61	36.05956327723922	38.0	37.0	38.0	34.0	38.0
62-63	36.04579154251822	38.0	37.0	38.0	34.0	38.0
64-65	36.07021556366892	38.0	37.0	38.0	34.0	38.0
66-67	35.993075664106755	38.0	37.0	38.0	33.5	38.0
68-69	35.68909032411487	38.0	37.0	38.0	31.5	38.0
70-71	35.9293577853316	38.0	37.0	38.0	32.5	38.0
72-73	35.929553625134645	38.0	37.0	38.0	32.0	38.0
74-75	35.937121434666444	38.0	37.0	38.0	32.0	38.0
76	34.81230153734246	37.0	35.0	38.0	27.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	13.0
14	50.0
15	94.0
16	186.0
17	223.0
18	260.0
19	241.0
20	193.0
21	175.0
22	191.0
23	223.0
24	227.0
25	305.0
26	328.0
27	390.0
28	523.0
29	669.0
30	803.0
31	1138.0
32	1450.0
33	2075.0
34	3090.0
35	5279.0
36	11156.0
37	42204.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.19003455173668	18.529243079161244	9.97524025347266	26.305482115629413
2	23.08811392281114	20.683480912613483	31.12314127044078	25.1052638941346
3	19.579783736903213	21.806762068627865	28.926937764908306	29.686516429560623
4	13.728370193181977	16.254703652412328	36.32268804118231	33.694238113223385
5	13.696196511253794	33.5739365199267	35.523941415921776	17.20592555289773
6	29.746667226208963	36.77451844391288	18.536237357841287	14.942576972036875
7	28.715710548771106	29.48088463636745	21.897687691468377	19.905717123393067
8	25.30809797585575	34.1726467749381	19.62874368766349	20.890511561542656
9	24.011358708576385	16.502301117685732	21.513002364066196	37.97333780967168
10-11	24.976569166421868	24.448501126078867	27.179067522766374	23.395862184732888
12-13	25.030425112258175	23.24408633737603	26.179585099388703	25.545903450977104
14-15	23.20771608823982	23.94561248898401	23.84489487599144	29.00177654678473
16-17	23.23219606361996	26.730034831507826	25.120651307230684	24.91711779764153
18-19	22.718816008505044	26.762907941304015	26.277505000909258	24.240771049281687
20-21	22.68454404297285	26.655196049631403	26.30618154349742	24.35407836389833
22-23	22.951026060682363	26.48383622197043	26.344650076237635	24.22048764110957
24-25	22.65306978891267	26.446766544966216	26.333459230349575	24.566704435771538
26-27	22.203337669786116	26.536293312070725	26.68527144795557	24.575097570187587
28-29	22.700630883936938	26.50342020227454	26.92447577881293	23.87147313497559
30-31	22.99858715570663	26.38871403192189	26.560773287450868	24.051925524920613
32-33	22.624393246324505	26.85733070348455	26.37122833522179	24.147047714969155
34-35	23.11049561458727	25.92009736035923	26.951753465665085	24.01765355938842
36-37	23.31332969630842	26.472645376082365	26.037601242183893	24.176423685425323
38-39	22.564242449676165	26.723040552827786	26.659392616839426	24.053324380656623
40-41	22.72511085931708	25.913103081679186	27.334340509463257	24.027445549540477
42-43	22.538363618560016	26.304782687761413	26.78808734455216	24.368766349126414
44-45	22.3061535663827	26.232042189488997	26.82375816582036	24.638046078307944
46-47	22.60341041028439	26.00262984878369	26.89020381328074	24.50375592765118
48-49	22.076041797809392	25.84106201127478	27.300068543931065	24.782827646984767
50-51	22.18095597800999	26.00193042091569	27.25740344398282	24.559710157091498
52-53	21.992110453648916	25.30949683159176	27.946339893966737	24.752052820792592
54-55	21.6018297033027	25.822876886706673	27.716927553261435	24.858365856729193
56-57	21.222739798844543	25.83826429980276	27.93514904807867	25.00384685327402
58-59	21.638199952438907	25.688586736049913	27.544868297732457	25.12834501377873
60-61	21.431868731377733	25.645921636101672	27.647684194329038	25.27452543819156
62-63	20.78699623707807	25.47875837564872	28.345013778729	25.38923160854421
64-65	21.53538405584232	25.58716969518933	27.514792899408285	25.36265334956006
66-67	21.41368360680963	25.37244533971211	27.277686852154936	25.93618420132332
68-69	21.069565095751674	25.176605536671005	27.43785583392785	26.315973533649473
70-71	21.872508288220235	25.431197280624453	27.077650481905803	25.618643949249513
72-73	22.062752668317316	25.22206834809126	26.59014925790703	26.12502972568439
74-75	21.73821813756347	25.735448403206178	26.747520528207925	25.778812931022422
76	22.716018297033028	25.345867080727963	26.354442066389694	25.58367255584931
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	0.5
7	0.0
8	0.0
9	0.5
10	1.0
11	1.0
12	1.0
13	0.5
14	3.0
15	7.5
16	9.0
17	9.0
18	15.5
19	40.5
20	75.0
21	91.0
22	93.5
23	134.5
24	219.0
25	265.0
26	310.0
27	396.0
28	543.0
29	649.0
30	715.0
31	888.0
32	1167.0
33	1339.0
34	1519.0
35	1884.5
36	2274.5
37	2479.0
38	2649.0
39	3154.0
40	3775.0
41	4083.0
42	4105.0
43	4160.0
44	4200.0
45	4173.0
46	4161.0
47	4081.0
48	3803.5
49	3420.0
50	3234.0
51	3004.0
52	2467.5
53	2023.0
54	1885.0
55	1720.0
56	1456.5
57	1271.5
58	1185.0
59	1083.0
60	923.5
61	790.5
62	715.0
63	716.0
64	741.5
65	762.0
66	725.0
67	692.0
68	707.0
69	727.5
70	692.5
71	652.0
72	670.0
73	635.5
74	550.5
75	518.0
76	487.5
77	412.5
78	329.0
79	290.0
80	255.0
81	186.0
82	122.0
83	92.0
84	80.5
85	57.5
86	34.5
87	23.0
88	21.5
89	14.0
90	10.0
91	7.5
92	3.0
93	2.5
94	1.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	71487.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.577811350315436
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.20733206661451	47.06310238225132
2	13.769978763831453	17.23390266761789
3	4.385827651726835	8.233664862142767
4	2.1772661227227004	5.4499419474869555
5	1.3367609254498714	4.182578650663757
6	0.7957974740136358	2.9879558521129717
7	0.6147311948139041	2.692797291815295
8	0.46272493573264784	2.3165050988291576
9	0.3151894489773108	1.7751479289940828
>10	0.9343914161171342	8.064403318085805
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0013988557360079454	0.0
5	0.0	0.0	0.0	0.0013988557360079454	0.0
6	0.0	0.0	0.0	0.0013988557360079454	0.0
7	0.0	0.0	0.0	0.0013988557360079454	0.0
8	0.0	0.0	0.0	0.0013988557360079454	0.0
9	0.0	0.0	0.0	0.0013988557360079454	0.0
10	0.0	0.0	0.0	0.0013988557360079454	0.0
11	0.0	0.0	0.0	0.0013988557360079454	0.0
12	0.0	0.0	0.0	0.0013988557360079454	0.0
13	0.0	0.0	0.0	0.0013988557360079454	0.0
14	0.0	0.0	0.0	0.0013988557360079454	0.0
15	0.0	0.0	0.0	0.0013988557360079454	0.0
16	0.0	0.0	0.0	0.0013988557360079454	0.0
17	0.0	0.0	0.0	0.0013988557360079454	0.0
18	0.0	0.0	0.0	0.0013988557360079454	0.0
19	0.0	0.0	0.0	0.0013988557360079454	0.0
20	0.0	0.0	0.0	0.0013988557360079454	0.0
21	0.0	0.0	0.0	0.0013988557360079454	0.0
22	0.0	0.0	0.0	0.008393134416047674	0.0
23	0.0	0.0	0.0	0.008393134416047674	0.0
24	0.0	0.0	0.0	0.011190845888063563	0.0
25	0.0	0.0	0.0	0.011190845888063563	0.0
26	0.0	0.0	0.0	0.011190845888063563	0.0
27	0.0	0.0	0.0	0.013988557360079455	0.0
28	0.0	0.0	0.0	0.01818512456810329	0.0
29	0.0	0.0	0.0	0.023780547512135073	0.0
30	0.0	0.0	0.0	0.023780547512135073	0.0
31	0.0	0.0	0.0	0.02797711472015891	0.0
32	0.0	0.0	0.0	0.04196567208023837	0.0
33	0.0	0.0	0.0	0.06294850812035754	0.0
34	0.0	0.0	0.0	0.08812791136850057	0.0
35	0.0	0.0	0.0	0.12309930476869921	0.0
36	0.0	0.0	0.0	0.17485696700099318	0.0
37	0.0	0.0	0.0	0.2881642816176368	0.0
38	0.0	0.0	0.0	0.4420384125785108	0.0
39	0.0	0.0	0.0	0.6001091107474086	0.0
40	0.0	0.0	0.0	0.8071397596765846	0.0
41	0.0	0.0	0.0	1.0281589659658399	0.0
42	0.0	0.0	0.0	1.3219186705275086	0.0
43	0.0	0.0	0.0	1.6212737980332088	0.0
44	0.0	0.0	0.0	2.0143522598514414	0.0
45	0.0	0.0	0.0	2.523535747758334	0.0
46	0.0	0.0	0.0	3.059297494649377	0.0
47	0.0	0.0	0.0	3.5796718284443325	0.0
48	0.0	0.0	0.0	4.1000461622392885	0.0
49	0.0	0.0	0.0	4.6218193517702515	0.0
50	0.0	0.0	0.0	5.108621147901017	0.0
51	0.0	0.0	0.0	5.606613789919845	0.0
52	0.0	0.0	0.0	6.073831605746499	0.0
53	0.0	0.0	0.0	6.566228824821296	0.0
54	0.0	0.0	0.0	7.1103837061283865	0.0
55	0.0	0.0	0.0	7.721683662763859	0.0
56	0.0	0.0	0.0	8.331584763663324	0.0
57	0.0	0.0	0.0	8.889728202330494	0.0
58	0.0	0.0	0.0	9.547190398254228	0.0
59	0.0	0.0	0.0	10.155692643417684	0.0
60	0.0	0.0	0.0	10.711038370612838	0.0013988557360079454
61	0.0	0.0	0.0	11.284569222376096	0.0013988557360079454
62	0.0	0.0	0.0	11.834319526627219	0.0013988557360079454
63	0.0	0.0	0.0	12.386867542350357	0.0013988557360079454
64	0.0	0.0	0.0	12.96459496132164	0.0013988557360079454
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAAGTAC	15	0.0022163512	70.0	40
TGCGGCA	15	0.0022163512	70.0	35
TTACGTT	15	0.0022163512	70.0	52
TATGTTC	15	0.0022163512	70.0	28
TTTTACG	15	0.0022163512	70.0	50
GGAATGG	20	0.006925517	52.5	10
TGCTTAC	20	0.006925517	52.5	7
TCCGACA	20	0.006925517	52.5	66
CTGAGTC	20	0.006925517	52.5	40
TTACTCG	20	0.006925517	52.5	19
CGCCTAG	20	0.006925517	52.5	16
TACTGTA	20	0.006925517	52.5	50
TAATTCA	20	0.006925517	52.5	28
GCTTACC	20	0.006925517	52.5	8
GTATCCG	20	0.006925517	52.5	63
TATAGTC	20	0.006925517	52.5	5
CAAGAGT	20	0.006925517	52.5	4
GGTCTAC	20	0.006925517	52.5	1
ACAAGCA	20	0.006925517	52.5	34
TACGTTG	20	0.006925517	52.5	53
>>END_MODULE