FastQCFastQC Report
Wed 25 May 2016
SRR1781143_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781143_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68633
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT3970.5784389433654364No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATAC150.002215972970.043
CTTTGCG150.002215972970.039
AATCTCG850.057.6470636
GTCGCAT252.375168E-455.99999664
TATGCCG950.055.2631643
TCGTATG950.055.2631640
GCCGTCT950.055.2631646
TCTCGTA900.054.44444738
GAATGCA200.006924343352.52
ATTGTCG200.006924343352.561
TACGACA200.006924343352.549
AGACCCT200.006924343352.531
TGCCTAC200.006924343352.545
ATAAACG200.006924343352.523
CTGATGC200.006924343352.538
GATTAAC200.006924343352.58
AAAATCG200.006924343352.565
CGTATGC1000.052.49999641
CTCGTAT1000.052.49999639
CCGTCTT1000.052.49999647
Produced by FastQC (version 0.11.5)