##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781143_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	68633
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.79993589089796	34.0	34.0	34.0	31.0	34.0
2	32.87852782189326	34.0	34.0	34.0	31.0	34.0
3	32.99214663500066	34.0	34.0	34.0	31.0	34.0
4	33.1258141127446	34.0	34.0	34.0	31.0	34.0
5	33.05685311730509	34.0	34.0	34.0	31.0	34.0
6	36.08296300613407	38.0	36.0	38.0	32.0	38.0
7	35.93787245202745	38.0	36.0	38.0	32.0	38.0
8	35.59812335174042	38.0	36.0	38.0	31.0	38.0
9	35.61750178485568	38.0	36.0	38.0	31.0	38.0
10-11	35.573310215202596	37.5	36.0	38.0	31.0	38.0
12-13	35.510344877828445	37.0	36.0	38.0	31.0	38.0
14-15	35.52782917838358	38.0	36.0	38.0	31.0	38.0
16-17	35.43338481488497	37.0	35.5	38.0	31.0	38.0
18-19	35.44680401556103	37.0	36.0	38.0	31.0	38.0
20-21	35.363746302798944	37.0	35.0	38.0	31.0	38.0
22-23	35.443715122462955	37.0	36.0	38.0	31.0	38.0
24-25	35.41464018766483	37.0	36.0	38.0	31.0	38.0
26-27	35.14444217796104	37.0	35.0	38.0	28.5	38.0
28-29	35.07697463319394	37.0	35.0	38.0	27.0	38.0
30-31	35.12326431891364	37.0	35.0	38.0	27.0	38.0
32-33	35.03416723733481	37.0	35.0	38.0	27.0	38.0
34-35	35.085112118077305	37.0	35.0	38.0	27.0	38.0
36-37	34.997654189675515	37.0	35.0	38.0	27.0	38.0
38-39	35.00558040592718	37.0	35.0	38.0	27.0	38.0
40-41	35.02734107499308	37.0	35.0	38.0	27.0	38.0
42-43	34.973190739148805	37.0	35.0	38.0	27.0	38.0
44-45	34.957535005026735	37.0	35.0	38.0	27.0	38.0
46-47	34.97543455772005	37.0	35.0	38.0	27.0	38.0
48-49	34.97217082161643	37.0	35.0	38.0	27.0	38.0
50-51	34.84280885288418	37.0	34.5	38.0	27.0	38.0
52-53	34.86120379409322	37.0	35.0	38.0	27.0	38.0
54-55	34.86325819940845	37.0	35.0	38.0	27.0	38.0
56-57	34.78110384217505	37.0	34.0	38.0	27.0	38.0
58-59	34.77703145717075	37.0	34.0	38.0	27.0	38.0
60-61	34.803534742762224	37.0	34.0	38.0	27.0	38.0
62-63	34.74094094677487	37.0	34.0	38.0	27.0	38.0
64-65	34.65407311351682	37.0	34.0	38.0	27.0	38.0
66-67	34.634476126644614	37.0	34.0	38.0	27.0	38.0
68-69	34.55804787784302	37.0	34.0	38.0	26.5	38.0
70-71	34.53061209622193	37.0	34.0	38.0	26.5	38.0
72-73	34.51322250229481	37.0	34.0	38.0	26.0	38.0
74-75	34.506687744962335	37.0	34.0	38.0	26.0	38.0
76	33.390744976906156	37.0	34.0	38.0	23.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	1.0
20	2.0
21	2.0
22	20.0
23	54.0
24	114.0
25	200.0
26	381.0
27	673.0
28	1071.0
29	1720.0
30	2378.0
31	3441.0
32	4616.0
33	5973.0
34	7637.0
35	9695.0
36	12874.0
37	17779.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.514067576821645	18.826220622732503	9.476490900878586	25.183220899567267
2	24.868503489574987	22.107441026911253	29.31242988066965	23.711625602844112
3	21.588740110442497	23.038480031471742	29.04725132225023	26.32552853583553
4	15.6061952704967	18.370171783252953	35.35471274751213	30.668920198738213
5	14.720324042370288	34.55334897206883	34.51838037095858	16.207946614602307
6	30.75634170151385	37.250302332697096	18.13704777585127	13.856308189937785
7	29.612577040199323	29.80053327116693	21.307534276514215	19.279355412119532
8	26.213337607273466	34.97880028557691	19.330351288738655	19.477510818410966
9	25.486282109189457	16.713534305654715	20.79903253536928	37.00115104978654
10-11	25.880407384202936	25.17083618667405	26.91416665452479	22.034589774598228
12-13	25.935774335960836	23.09020442061399	26.147042967668614	24.826978275756563
14-15	24.02634301283639	23.842757857007562	23.525854909445894	28.605044220710152
16-17	24.55742864219836	26.971719143852084	24.61133856891	23.85951364503956
18-19	23.748779741523755	26.670843471799284	26.012996663412647	23.56738012326432
20-21	23.46684539507234	27.11377908586249	25.55111972374805	23.868255795317122
22-23	24.13197732869028	26.61256246994886	25.81192720702869	23.443532994332173
24-25	24.296621158917723	26.636603383212158	25.67350982763394	23.393265630236186
26-27	23.372867279588537	26.829659201841682	26.573222793699824	23.22425072486996
28-29	23.798318593096614	26.21115206970408	27.413197732869026	22.57733160433028
30-31	23.665000801363774	26.091676015910714	26.931650955079917	23.311672227645595
32-33	23.09384698322964	26.75607943700552	26.82747366427229	23.322599915492546
34-35	23.819445456267392	26.285460347063367	27.337432430463483	22.55766176620576
36-37	24.15237567933793	26.869727390613846	25.783515218626608	23.19438171142162
38-39	23.349554878848366	26.716739760756486	26.786676962976998	23.14702839741815
40-41	23.497442921043813	26.147771480191746	27.032185683271898	23.322599915492546
42-43	24.027800037882653	25.646554864278116	26.706540585432663	23.61910451240657
44-45	23.10914574621538	26.4522897148602	27.01761543280929	23.420949106115135
46-47	23.818716943744263	25.55184823627118	26.871184415660103	23.75825040432445
48-49	23.295644952136726	25.363892005303573	27.215770839100724	24.124692203458977
50-51	23.09311847070651	25.44257135780164	27.34981714335669	24.114493028135154
52-53	22.625413430856877	25.324552329054534	28.45132807832967	23.59870616175892
54-55	22.141681115498375	25.74126149228505	27.82043623329885	24.296621158917723
56-57	22.66038203196713	25.564961461687524	27.41028368277651	24.364372823568836
58-59	22.140952602975243	25.410516806783907	28.089257354333917	24.359273235906926
60-61	22.63706963122696	25.759474305363312	27.399355994929554	24.204100068480177
62-63	21.45177975609401	26.10406072880393	27.87726021010301	24.566899304999055
64-65	21.845905031107485	26.418778138796206	26.909067066862878	24.82624976323343
66-67	21.825506680459835	26.238107033059897	26.777934812699428	25.158451473780836
68-69	21.50423265775939	26.582693456500518	26.666472396660502	25.246601489079595
70-71	21.529002083545816	26.2213512450279	26.64898809610537	25.60065857532091
72-73	21.628079786691533	25.57734617458074	26.53971121763583	26.254862821091894
74-75	21.629536811737793	25.405417219121993	26.584150481546775	26.38089548759343
76	21.797094692057755	25.490653184328238	26.424606238981248	26.287645884632756
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	0.5
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	1.5
15	3.5
16	12.0
17	20.0
18	24.5
19	36.0
20	54.0
21	65.0
22	83.5
23	139.0
24	233.0
25	290.0
26	296.0
27	360.5
28	489.5
29	560.0
30	656.5
31	866.5
32	1192.0
33	1404.0
34	1512.5
35	1746.0
36	2103.0
37	2335.0
38	2587.0
39	3086.0
40	3611.5
41	3910.5
42	3931.0
43	3996.5
44	4066.5
45	4043.5
46	4016.0
47	3943.0
48	3604.5
49	3186.5
50	3034.0
51	2851.5
52	2399.0
53	1989.0
54	1849.0
55	1696.5
56	1427.5
57	1223.5
58	1136.0
59	1011.0
60	819.5
61	746.0
62	739.0
63	690.5
64	693.5
65	755.5
66	733.0
67	700.0
68	706.0
69	676.0
70	660.0
71	680.0
72	647.0
73	594.5
74	552.5
75	530.0
76	492.0
77	398.5
78	307.0
79	271.0
80	239.0
81	170.5
82	115.0
83	96.0
84	77.0
85	48.5
86	32.5
87	26.0
88	18.0
89	7.0
90	4.5
91	3.0
92	1.0
93	0.5
94	1.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	68633.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.05179724039456
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.9435796358098	50.05318141418851
2	12.894483391941227	16.776186382643917
3	4.316079467825386	8.423061792432211
4	2.150200461397182	5.594976177640493
5	1.0907787757296123	3.5478559876444278
6	0.7637691222254575	2.9810732446490755
7	0.47259614307792236	2.1520259933268253
8	0.3606065357134858	1.8766482595835823
9	0.27773422626380273	1.6260399516267683
>10	0.7279324478688377	6.390511852898752
>50	0.0	0.0
>100	0.0022397921472887316	0.5784389433654364
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT	397	0.5784389433654364	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0014570250462605451	0.0
5	0.0	0.0	0.0	0.0014570250462605451	0.0
6	0.0	0.0	0.0	0.0014570250462605451	0.0
7	0.0	0.0	0.0	0.0014570250462605451	0.0
8	0.0	0.0	0.0	0.0014570250462605451	0.0
9	0.0	0.0	0.0	0.0014570250462605451	0.0
10	0.0	0.0	0.0	0.0014570250462605451	0.0
11	0.0	0.0	0.0	0.0014570250462605451	0.0
12	0.0	0.0	0.0	0.0014570250462605451	0.0
13	0.0	0.0	0.0	0.0014570250462605451	0.0
14	0.0	0.0	0.0	0.0014570250462605451	0.0
15	0.0	0.0	0.0	0.0014570250462605451	0.0
16	0.0	0.0	0.0	0.0014570250462605451	0.0
17	0.0	0.0	0.0	0.0014570250462605451	0.0
18	0.0	0.0	0.0	0.0014570250462605451	0.0
19	0.0	0.0	0.0	0.0014570250462605451	0.0
20	0.0	0.0	0.0	0.0014570250462605451	0.0
21	0.0	0.0	0.0	0.0014570250462605451	0.0
22	0.0	0.0	0.0	0.0014570250462605451	0.0
23	0.0	0.0	0.0	0.0014570250462605451	0.0
24	0.0	0.0	0.0	0.0014570250462605451	0.0
25	0.0	0.0	0.0	0.0014570250462605451	0.0
26	0.0	0.0	0.0	0.0014570250462605451	0.0
27	0.0	0.0	0.0	0.004371075138781636	0.0
28	0.0	0.0	0.0	0.008742150277563272	0.0
29	0.0	0.0	0.0	0.013113225416344907	0.0
30	0.0	0.0	0.0	0.013113225416344907	0.0
31	0.0	0.0	0.0	0.016027275508866	0.0
32	0.0	0.0	0.0	0.03059752597147145	0.0
33	0.0	0.0	0.0	0.050995876619119085	0.0
34	0.0	0.0	0.0	0.07285125231302726	0.0
35	0.0	0.0	0.0	0.11073390351580144	0.0
36	0.0	0.0	0.0	0.16318680518118106	0.0
37	0.0	0.0	0.0	0.27246368365072193	0.0
38	0.0	0.0	0.0	0.4254513135080792	0.0
39	0.0	0.0	0.0	0.5784389433654364	0.0
40	0.0	0.0	0.0	0.7853364999344339	0.0
41	0.0	0.0	0.0	0.9936910815496919	0.0
42	0.0	0.0	0.0	1.2836390657555403	0.0
43	0.0	0.0	0.0	1.557559774452523	0.0
44	0.0	0.0	0.0	1.9349292614340041	0.0
45	0.0	0.0	0.0	2.4244896769775472	0.0
46	0.0	0.0	0.0	2.921335217752393	0.0
47	0.0	0.0	0.0	3.415266708434718	0.0
48	0.0	0.0	0.0	3.9325105998572116	0.0
49	0.0	0.0	0.0	4.448297466233445	0.0
50	0.0	0.0	0.0	4.910174405898037	0.0
51	0.0	0.0	0.0	5.379336470793933	0.0
52	0.0	0.0	0.0	5.832471260180962	0.0
53	0.0	0.0	0.0	6.303090350123119	0.0
54	0.0	0.0	0.0	6.8363615170544785	0.0
55	0.0	0.0	0.0	7.432284760975041	0.0
56	0.0	0.0	0.0	8.012180729386738	0.0
57	0.0	0.0	0.0	8.57459239724331	0.0
58	0.0	0.0	0.0	9.206941267320385	0.0
59	0.0	0.0	0.0	9.811606661518512	0.0
60	0.0	0.0	0.0	10.359448078912477	0.0
61	0.0	0.0	0.0	10.894176270890096	0.0
62	0.0	0.0	0.0	11.430361487913977	0.0
63	0.0	0.0	0.0	11.968003729984119	0.0
64	0.0	0.0	0.0	12.549356723442076	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTATAC	15	0.0022159729	70.0	43
CTTTGCG	15	0.0022159729	70.0	39
AATCTCG	85	0.0	57.64706	36
GTCGCAT	25	2.375168E-4	55.999996	64
TATGCCG	95	0.0	55.26316	43
TCGTATG	95	0.0	55.26316	40
GCCGTCT	95	0.0	55.26316	46
TCTCGTA	90	0.0	54.444447	38
GAATGCA	20	0.0069243433	52.5	2
ATTGTCG	20	0.0069243433	52.5	61
TACGACA	20	0.0069243433	52.5	49
AGACCCT	20	0.0069243433	52.5	31
TGCCTAC	20	0.0069243433	52.5	45
ATAAACG	20	0.0069243433	52.5	23
CTGATGC	20	0.0069243433	52.5	38
GATTAAC	20	0.0069243433	52.5	8
AAAATCG	20	0.0069243433	52.5	65
CGTATGC	100	0.0	52.499996	41
CTCGTAT	100	0.0	52.499996	39
CCGTCTT	100	0.0	52.499996	47
>>END_MODULE