FastQCFastQC Report
Wed 25 May 2016
SRR1781142_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781142_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38036
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTTT200.006900678452.521
CCCCGGG200.006900678452.51
TATTAAT200.006900678452.566
AAACAAC200.006900678452.527
GAGCCCT200.006900678452.518
CTGGACA200.006900678452.54
GAGTAGC350.001240510740.0000046
CCTGGAC400.002390213535.03
AATCACC500.007124338827.99999842
TCTAGAG500.007124338827.9999982
Produced by FastQC (version 0.11.5)