##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781142_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	38036
Sequences flagged as poor quality	0
Sequence length	76
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.27523924702913	34.0	34.0	34.0	27.0	34.0
2	32.489378483541905	34.0	34.0	34.0	31.0	34.0
3	32.55142496582185	34.0	34.0	34.0	31.0	34.0
4	32.59446314018299	34.0	34.0	34.0	31.0	34.0
5	32.59930066253023	34.0	34.0	34.0	31.0	34.0
6	35.84067725312862	38.0	37.0	38.0	32.0	38.0
7	35.668945209801244	38.0	37.0	38.0	31.0	38.0
8	35.63205910190346	38.0	37.0	38.0	31.0	38.0
9	35.8724366389736	38.0	37.0	38.0	32.0	38.0
10-11	35.78045798717005	38.0	37.0	38.0	31.0	38.0
12-13	35.81188873698601	38.0	37.0	38.0	31.5	38.0
14-15	35.783284257019666	38.0	37.0	38.0	31.5	38.0
16-17	35.76898201703649	38.0	37.0	38.0	31.0	38.0
18-19	35.76172573351562	38.0	37.0	38.0	31.0	38.0
20-21	35.756717320433275	38.0	37.0	38.0	31.0	38.0
22-23	35.80422494478914	38.0	37.0	38.0	31.5	38.0
24-25	35.78662319907456	38.0	37.0	38.0	31.5	38.0
26-27	35.739431065306555	38.0	37.0	38.0	31.0	38.0
28-29	35.7835077295194	38.0	37.0	38.0	31.5	38.0
30-31	35.8663371542749	38.0	37.0	38.0	32.0	38.0
32-33	35.83758807445578	38.0	37.0	38.0	32.0	38.0
34-35	35.861657377221576	38.0	37.0	38.0	32.0	38.0
36-37	35.7738326848249	38.0	37.0	38.0	31.5	38.0
38-39	35.75792670102008	38.0	37.0	38.0	31.0	38.0
40-41	35.71296929224945	38.0	37.0	38.0	31.0	38.0
42-43	35.82218161741508	38.0	37.0	38.0	32.0	38.0
44-45	35.8133084446314	38.0	37.0	38.0	32.0	38.0
46-47	35.77897255231886	38.0	37.0	38.0	31.5	38.0
48-49	35.77076979703439	38.0	37.0	38.0	31.5	38.0
50-51	35.6765432747923	38.0	37.0	38.0	31.0	38.0
52-53	35.73899726574824	38.0	37.0	38.0	31.0	38.0
54-55	35.70117783152803	38.0	37.0	38.0	31.0	38.0
56-57	35.627037543379956	38.0	37.0	38.0	31.0	38.0
58-59	35.637277842044384	38.0	37.0	38.0	31.0	38.0
60-61	35.66401566936587	38.0	37.0	38.0	31.0	38.0
62-63	35.65654905878641	38.0	37.0	38.0	31.0	38.0
64-65	35.62828636029025	38.0	37.0	38.0	31.0	38.0
66-67	35.56420233463035	38.0	37.0	38.0	31.0	38.0
68-69	35.31345304448418	38.0	37.0	38.0	29.0	38.0
70-71	35.49755494794405	38.0	37.0	38.0	31.0	38.0
72-73	35.53712272583868	38.0	37.0	38.0	31.0	38.0
74-75	35.555776106846146	38.0	37.0	38.0	31.0	38.0
76	34.61441791986539	37.0	35.0	38.0	26.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	4.0
13	15.0
14	47.0
15	90.0
16	151.0
17	179.0
18	200.0
19	151.0
20	155.0
21	146.0
22	168.0
23	157.0
24	205.0
25	228.0
26	271.0
27	310.0
28	367.0
29	447.0
30	538.0
31	662.0
32	862.0
33	1277.0
34	1689.0
35	2738.0
36	5325.0
37	21654.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.8883163318961	19.94952150594174	10.006309811757284	27.15585235040488
2	21.966032180039964	19.615627300452203	33.07130087285729	25.347039646650543
3	18.627090125144598	21.195709328005048	29.64034072983489	30.53685981701546
4	12.290987485540015	16.02429277526554	36.986013250604685	34.69870648858976
5	12.248922073824797	33.91786728362604	37.16742033862657	16.665790303922602
6	29.19865390682511	38.25586286675781	18.658639183931015	13.886844042486066
7	27.30571037964034	30.252918287937742	22.310442738458303	20.130928593963613
8	23.929961089494164	36.065832369334316	19.542012829950572	20.462193711220948
9	22.888842149542537	14.778104953202229	21.703123356819855	40.62992954043538
10-11	23.20696182563887	24.885634661899253	28.78983068671785	23.11757282574403
12-13	23.91944473656536	23.181985487432957	28.049742349353245	24.848827426648437
14-15	21.52434535703018	24.496529603533496	24.87774739720265	29.101377642233672
16-17	21.66368703333684	28.124671363970975	25.966189925333893	24.245451677358293
18-19	21.02350404879588	27.977442422967712	27.473972026501208	23.525081501735198
20-21	21.34951098958881	27.589651908718054	27.297823114943736	23.763013986749396
22-23	21.383689136607426	27.005994321169418	27.54890104111894	24.061415501104218
24-25	21.39157640130403	27.392470291302978	27.090125144599853	24.12582816279314
26-27	20.644915343358925	27.35434851193606	28.11284046692607	23.887895677778946
28-29	20.8328951519613	27.614628246923967	28.4322746871385	23.12020191397623
30-31	21.264065622042274	28.136502261015877	27.655379114523083	22.94405300241876
32-33	20.44773372594384	27.857818908402564	27.67115364391629	24.0232937217373
34-35	21.140498475128826	27.324113997265748	27.807866231990747	23.727521295614682
36-37	21.695236092123253	27.64486276159428	27.071721526974446	23.588179619308026
38-39	20.79082974024608	28.028709643495635	28.1838258491955	22.996634767062783
40-41	21.111578504574613	27.213692291513304	28.405983804816486	23.268745399095593
42-43	20.840782416657902	27.67772636449679	27.785518982017038	23.69597223682827
44-45	20.78294247554948	27.991902408244822	27.583079188137553	23.642075928068145
46-47	20.521348196445473	27.55415921758334	27.89199705542118	24.032495530550005
48-49	20.72641707855716	27.611999158691763	28.122042275738774	23.539541487012304
50-51	20.963035019455255	27.529182879377434	27.931433378904195	23.57634872226312
52-53	20.905195078346832	27.333315806078453	28.13518771689978	23.62630139867494
54-55	20.165106740982228	28.09180776106846	28.1877694815438	23.555316016405513
56-57	20.71327163739615	27.42007571774109	28.298191187296247	23.568461457566517
58-59	20.63176990219792	27.238668629719214	28.509832789988433	23.619728678094436
60-61	20.36623199074561	27.828898937848358	28.198285834472603	23.60658323693343
62-63	20.211115785045745	27.57650646755705	28.877905142496584	23.334472604900622
64-65	20.53317909349038	27.915658849510987	28.093122305184565	23.458039751814074
66-67	20.205857608581343	27.86439162898307	28.06420233463035	23.865548427805237
68-69	20.21505941739405	27.266274056157325	28.377063834262277	24.14160269218635
70-71	20.815806078451992	27.848617099589863	27.88673887895678	23.448837943001365
72-73	20.846040593122307	27.229466820906513	27.88805342307288	24.036439162898308
74-75	20.431959196550636	27.765800820275526	27.998475128825323	23.80376485434851
76	20.81449153433589	27.153223262172677	28.06025870228205	23.97202650120938
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	1.0
8	1.0
9	1.5
10	2.0
11	1.0
12	0.0
13	1.0
14	4.0
15	10.5
16	18.0
17	21.0
18	22.0
19	40.0
20	61.5
21	66.0
22	83.0
23	127.5
24	189.0
25	223.0
26	260.5
27	349.0
28	469.0
29	538.0
30	581.5
31	664.0
32	814.5
33	926.0
34	1018.0
35	1190.0
36	1399.0
37	1528.0
38	1608.5
39	1814.5
40	1998.0
41	2097.0
42	2138.0
43	2159.0
44	2182.5
45	2145.0
46	2105.0
47	2082.0
48	1958.5
49	1767.5
50	1677.0
51	1516.5
52	1294.5
53	1200.5
54	1168.0
55	1104.5
56	962.0
57	808.5
58	734.0
59	646.5
60	525.5
61	480.5
62	469.0
63	395.5
64	303.0
65	257.0
66	217.5
67	205.0
68	196.0
69	172.5
70	146.5
71	135.0
72	129.5
73	114.0
74	91.0
75	78.0
76	77.5
77	72.5
78	54.0
79	40.0
80	39.5
81	33.0
82	27.0
83	27.0
84	18.5
85	9.5
86	5.5
87	2.0
88	4.5
89	5.0
90	2.0
91	1.0
92	1.0
93	1.5
94	1.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	38036.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.40919129245978
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.55985273371641	63.847407719003044
2	10.690568764408354	16.33715427489747
3	2.8008120290403604	6.420233463035019
4	1.1251419330420123	3.4388474077190025
5	0.6537521935106493	2.4976338205910187
6	0.36816570897704987	1.6878746450730886
7	0.2855864845335994	1.5275002629088232
8	0.15483604583146957	0.9464717635923862
9	0.11010563259126725	0.757177410873909
>10	0.2511784743488284	2.539699232306236
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0026290882322010725	0.0
20	0.0	0.0	0.0	0.0026290882322010725	0.0
21	0.0	0.0	0.0	0.0026290882322010725	0.0
22	0.0	0.0	0.0	0.005258176464402145	0.0
23	0.0	0.0	0.0	0.005258176464402145	0.0
24	0.0	0.0	0.0	0.015774529393206435	0.0
25	0.0	0.0	0.0	0.015774529393206435	0.0
26	0.0	0.0	0.0	0.015774529393206435	0.0
27	0.0	0.0	0.0	0.015774529393206435	0.0
28	0.0	0.0	0.0	0.015774529393206435	0.0
29	0.0	0.0	0.0	0.015774529393206435	0.0
30	0.0	0.0	0.0	0.018403617625407508	0.0
31	0.0	0.0	0.0	0.02103270585760858	0.0
32	0.0	0.0	0.0	0.026290882322010725	0.0
33	0.0	0.0	0.0	0.03154905878641287	0.0
34	0.0	0.0	0.0	0.03943632348301609	0.0
35	0.0	0.0	0.0	0.047323588179619305	0.0
36	0.0	0.0	0.0	0.05521085287622252	0.0
37	0.0	0.0	0.0	0.07624355873383111	0.0
38	0.0	0.0	0.0	0.12882532337785257	0.0
39	0.0	0.0	0.0	0.17614891155747187	0.0
40	0.0	0.0	0.0	0.23398885266589547	0.0
41	0.0	0.0	0.0	0.3076033231675255	0.0
42	0.0	0.0	0.0	0.3707014407403513	0.0
43	0.0	0.0	0.0	0.46797770533179095	0.0
44	0.0	0.0	0.0	0.5810284993164371	0.0
45	0.0	0.0	0.0	0.6967083815332843	0.0
46	0.0	0.0	0.0	0.870228204858555	0.0
47	0.0	0.0	0.0	1.0437480281838258	0.0
48	0.0	0.0	0.0	1.2146387632768956	0.0
49	0.0	0.0	0.0	1.3592386160479546	0.0
50	0.0	0.0	0.0	1.488063939425807	0.0
51	0.0	0.0	0.0	1.622147439268062	0.0
52	0.0	0.0	0.0	1.7667472920391207	0.0
53	0.0	0.0	0.0	1.919234409506783	0.0
54	0.0	0.0	0.0	2.0690924387422442	0.0
55	0.0	0.0	0.0	2.245241350299716	0.0
56	0.0	0.0	0.0	2.4345357030181933	0.0
57	0.0	0.0	0.0	2.529182879377432	0.0
58	0.0	0.0	0.0	2.707960879167105	0.0
59	0.0	0.0	0.0	2.9156588495109896	0.0
60	0.0	0.0	0.0	3.115469555158271	0.0
61	0.0	0.0	0.0	3.3389420548953623	0.0
62	0.0	0.0	0.0	3.48091281943422	0.0
63	0.0	0.0	0.0	3.646545378062888	0.0
64	0.0	0.0	0.0	3.8568724366389735	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATTTT	20	0.0069006784	52.5	21
CCCCGGG	20	0.0069006784	52.5	1
TATTAAT	20	0.0069006784	52.5	66
AAACAAC	20	0.0069006784	52.5	27
GAGCCCT	20	0.0069006784	52.5	18
CTGGACA	20	0.0069006784	52.5	4
GAGTAGC	35	0.0012405107	40.000004	6
CCTGGAC	40	0.0023902135	35.0	3
AATCACC	50	0.0071243388	27.999998	42
TCTAGAG	50	0.0071243388	27.999998	2
>>END_MODULE