FastQCFastQC Report
Wed 25 May 2016
SRR1781142_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781142_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36027
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT420.1165792322424848No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATACC200.00689773352.50000461
TAGCGTG200.00689773352.5000044
TAGAAGA200.00689773352.50000465
ATTGAGA200.00689773352.50000459
AAAGCCC200.00689773352.50000462
TACACAA305.803426E-446.6666645
ATCCTCC350.001239638340.035
GCATTAT350.001239638340.01
Produced by FastQC (version 0.11.5)