##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781142_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	36027
Sequences flagged as poor quality	0
Sequence length	76
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.628112249146476	34.0	34.0	34.0	31.0	34.0
2	32.78693757459683	34.0	34.0	34.0	31.0	34.0
3	32.93588142226663	34.0	34.0	34.0	31.0	34.0
4	33.12493407721986	34.0	34.0	34.0	31.0	34.0
5	32.99578094207122	34.0	34.0	34.0	31.0	34.0
6	35.89363532905876	38.0	36.0	38.0	32.0	38.0
7	35.84991811696783	38.0	36.0	38.0	31.0	38.0
8	35.45565825630777	37.0	36.0	38.0	31.0	38.0
9	35.47558775362922	37.0	36.0	38.0	31.0	38.0
10-11	35.49739084575457	37.0	36.0	38.0	31.0	38.0
12-13	35.40673661420601	37.0	35.5	38.0	31.0	38.0
14-15	35.46037693951759	37.0	36.0	38.0	31.0	38.0
16-17	35.33516529269714	37.0	35.0	38.0	31.0	38.0
18-19	35.371415882532546	37.0	35.5	38.0	31.0	38.0
20-21	35.28542482027368	37.0	35.0	38.0	30.5	38.0
22-23	35.31792822050129	37.0	35.0	38.0	31.0	38.0
24-25	35.34998750936798	37.0	35.0	38.0	31.0	38.0
26-27	35.07584589335776	37.0	35.0	38.0	27.0	38.0
28-29	35.05737363643934	37.0	35.0	38.0	27.0	38.0
30-31	35.07298692647181	37.0	35.0	38.0	27.0	38.0
32-33	34.98174979876204	37.0	35.0	38.0	27.0	38.0
34-35	34.95299358814223	37.0	35.0	38.0	27.0	38.0
36-37	34.94984317317567	37.0	35.0	38.0	27.0	38.0
38-39	34.968828933855164	37.0	35.0	38.0	27.0	38.0
40-41	34.95321564382269	37.0	35.0	38.0	27.0	38.0
42-43	34.896438782024596	37.0	35.0	38.0	27.0	38.0
44-45	34.87065256613096	37.0	35.0	38.0	27.0	38.0
46-47	34.915507813584256	37.0	34.5	38.0	27.0	38.0
48-49	34.85213867377245	37.0	35.0	38.0	27.0	38.0
50-51	34.794251533572044	37.0	34.0	38.0	27.0	38.0
52-53	34.78170538762595	37.0	34.0	38.0	27.0	38.0
54-55	34.81747023066034	37.0	34.0	38.0	27.0	38.0
56-57	34.734657340328084	37.0	34.0	38.0	27.0	38.0
58-59	34.72828711799484	37.0	34.0	38.0	27.0	38.0
60-61	34.736988924972934	37.0	34.0	38.0	27.0	38.0
62-63	34.67003913731368	37.0	34.0	38.0	27.0	38.0
64-65	34.603339162294944	37.0	34.0	38.0	27.0	38.0
66-67	34.64387820245927	37.0	34.0	38.0	27.0	38.0
68-69	34.58697643434091	37.0	34.0	38.0	27.0	38.0
70-71	34.56804618758153	37.0	34.0	38.0	26.5	38.0
72-73	34.59163960363061	37.0	34.0	38.0	27.0	38.0
74-75	34.62400421905793	37.0	34.0	38.0	27.0	38.0
76	33.684181308463096	37.0	34.0	38.0	24.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	5.0
21	5.0
22	8.0
23	19.0
24	58.0
25	113.0
26	196.0
27	365.0
28	537.0
29	840.0
30	1276.0
31	1796.0
32	2521.0
33	3230.0
34	4101.0
35	5389.0
36	7272.0
37	8295.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.63244233491548	21.336775196380493	9.501207427762512	23.529575040941516
2	22.372109806533988	23.37968745662975	31.57076636966719	22.67743636716907
3	20.74832764315652	25.36153440475199	29.580592333527633	24.309545618563856
4	14.644572126460709	17.88103366919255	38.15749299136758	29.316901212979158
5	13.853498764815278	37.052765981069754	35.30962888944403	13.784106364670942
6	31.18216892885891	39.32606100979821	17.708940516834595	11.782829544508285
7	29.06431287645377	31.25988841702057	22.383212590557083	17.29258611596858
8	25.09506758819774	37.10550420517945	19.29941432814278	18.50001387848003
9	24.892441779776277	16.087934049462906	21.619896188969385	37.399727981791436
10-11	25.115885308241044	26.177589030449383	28.406472923085463	20.30005273822411
12-13	25.03261442806784	24.297054986537876	27.915174730063562	22.755155855330724
14-15	23.225636328309324	25.652982485358205	24.61209648319316	26.509284703139315
16-17	23.09517861603797	29.5528353734699	25.272712132567243	22.07927387792489
18-19	22.78568851139423	28.321814194909372	27.204596552585556	21.687900741110834
20-21	22.93002470369445	28.41618785910567	27.056096816276682	21.597690620923196
22-23	22.87034723957032	28.10530990645905	27.233741360646178	21.790601493324452
24-25	23.04105254392539	27.725039553668086	27.436367169067644	21.797540733338884
26-27	22.542815110889055	27.908235490049133	27.79026840980376	21.758680989258057
28-29	22.39015183057152	27.987342826213673	28.21217420268132	21.41033114053349
30-31	23.0813001360091	28.028978266300275	27.935992450106866	20.953729147583758
32-33	22.83703888750104	28.40369722707969	27.440530713076306	21.318733172342966
34-35	23.258944680378605	27.41277375301857	27.94154384211841	21.386737724484416
36-37	23.00219279984456	28.174702306603383	27.210147944597107	21.61295694895495
38-39	22.73156243928165	28.398145835068146	27.923501818080883	20.946789907569325
40-41	22.541427262886167	28.169150914591835	28.056735226358008	21.232686596163987
42-43	22.535875870874623	27.922113970078	27.958198018153052	21.583812140894327
44-45	22.548366502900603	28.05257168234935	27.711161073639218	21.687900741110834
46-47	22.36794626252533	27.745857273711383	27.93044105809532	21.955755405667972
48-49	22.141727038054793	27.89990840203181	28.38981874705082	21.568545812862574
50-51	22.26940905432037	28.06922585838399	27.958198018153052	21.703167069142587
52-53	22.74821661531629	27.580703361367863	28.192744330640906	21.47833569267494
54-55	22.538651566880397	27.618175257445802	28.3190384989036	21.524134676770203
56-57	22.11813362200572	27.9110111860549	27.980403586199238	21.990451605740137
58-59	22.287451078357897	27.818025369861495	28.209398506675548	21.68512504510506
60-61	22.220834374219336	27.704221833624782	28.26768812279679	21.80725566935909
62-63	21.696227829128155	28.01371193826852	28.746495683792713	21.543564548810615
64-65	21.965470341688178	27.974852194187694	28.11502484247925	21.944652621644877
66-67	21.966858189691067	27.786104865795096	28.05118383434646	22.195853110167374
68-69	22.03208704582674	28.117800538485028	28.144169650539872	21.70594276514836
70-71	22.295778166375218	28.142781802536987	27.804146889832626	21.757293141255168
72-73	21.957143253670857	27.55294640131013	28.01926333028007	22.470647014738944
74-75	22.152829822077884	27.715324617647873	28.00954839425986	22.12229716601438
76	22.163932606100982	27.59041829738807	28.00954839425986	22.23610070225109
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	0.5
10	1.0
11	2.5
12	3.0
13	1.5
14	3.5
15	7.5
16	10.5
17	13.0
18	20.0
19	34.5
20	58.5
21	75.0
22	99.0
23	143.5
24	214.0
25	264.0
26	306.5
27	367.0
28	448.0
29	511.0
30	570.5
31	728.0
32	925.0
33	1024.0
34	1058.0
35	1183.5
36	1341.0
37	1407.0
38	1509.0
39	1677.0
40	1919.5
41	2082.0
42	2068.0
43	2081.5
44	2080.0
45	2033.0
46	2001.0
47	1959.5
48	1833.5
49	1657.0
50	1565.0
51	1431.0
52	1185.0
53	1039.0
54	1005.0
55	949.5
56	836.5
57	706.5
58	634.0
59	583.5
60	487.5
61	395.0
62	348.0
63	313.5
64	254.5
65	219.5
66	201.0
67	193.0
68	174.5
69	142.5
70	129.0
71	129.0
72	116.0
73	111.0
74	104.5
75	90.0
76	79.0
77	56.0
78	41.0
79	38.0
80	31.5
81	26.0
82	22.0
83	17.0
84	12.5
85	5.5
86	5.0
87	7.0
88	5.0
89	3.0
90	2.5
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	36027.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.51333721930774
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.60369087180938	66.42518111416437
2	10.019090716255391	15.732644960723901
3	2.8070423531075446	6.611707885752352
4	1.1313017040231916	3.552890887390013
5	0.5479742628862335	2.151164404474422
6	0.3393905112069575	1.5988008993255058
7	0.19444248037898607	1.0686429622227773
8	0.12020080605246411	0.7549893135703778
9	0.09191826345188432	0.6495128653509868
>10	0.14494803082797145	1.4544647070252865
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT	42	0.1165792322424848	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0027756960057734475	0.0
20	0.0	0.0	0.0	0.0027756960057734475	0.0
21	0.0	0.0	0.0	0.0027756960057734475	0.0
22	0.0	0.0	0.0	0.0027756960057734475	0.0
23	0.0	0.0	0.0	0.0027756960057734475	0.0
24	0.0	0.0	0.0	0.0027756960057734475	0.0
25	0.0	0.0	0.0	0.0027756960057734475	0.0
26	0.0	0.0	0.0	0.0027756960057734475	0.0
27	0.0	0.0	0.0	0.0027756960057734475	0.0
28	0.0	0.0	0.0	0.0027756960057734475	0.0
29	0.0	0.0	0.0	0.0027756960057734475	0.0
30	0.0	0.0	0.0	0.008327088017320343	0.0
31	0.0	0.0	0.0	0.01110278402309379	0.0
32	0.0	0.0	0.0	0.019429872040414135	0.0
33	0.0	0.0	0.0	0.02220556804618758	0.0
34	0.0	0.0	0.0	0.027756960057734478	0.0
35	0.0	0.0	0.0	0.030532656063507923	0.0
36	0.0	0.0	0.0	0.03330835206928137	0.0
37	0.0	0.0	0.0	0.07216809615010963	0.0
38	0.0	0.0	0.0	0.11380353623671136	0.0
39	0.0	0.0	0.0	0.15266328031753962	0.0
40	0.0	0.0	0.0	0.20817720043300858	0.0
41	0.0	0.0	0.0	0.27756960057734475	0.0
42	0.0	0.0	0.0	0.33863491270436064	0.0
43	0.0	0.0	0.0	0.421905792877564	0.0
44	0.0	0.0	0.0	0.5190551530796347	0.0
45	0.0	0.0	0.0	0.6245316012990257	0.0
46	0.0	0.0	0.0	0.7799705776223388	0.0
47	0.0	0.0	0.0	0.9492880339745191	0.0
48	0.0	0.0	0.0	1.1158297943209259	0.0
49	0.0	0.0	0.0	1.246287506592278	0.0
50	0.0	0.0	0.0	1.3711938268520831	0.0
51	0.0	0.0	0.0	1.4933244511061148	0.0
52	0.0	0.0	0.0	1.6348849474005607	0.0
53	0.0	0.0	0.0	1.77922113970078	0.0
54	0.0	0.0	0.0	1.9207816359952259	0.0
55	0.0	0.0	0.0	2.0762206123185387	0.0
56	0.0	0.0	0.0	2.2594165486995865	0.0
57	0.0	0.0	0.0	2.3565659089016573	0.0
58	0.0	0.0	0.0	2.5258833652538373	0.0
59	0.0	0.0	0.0	2.717406389652205	0.0
60	0.0	0.0	0.0	2.903378022039026	0.0
61	0.0	0.0	0.0	3.122658006495129	0.0
62	0.0	0.0	0.0	3.261442806783801	0.0
63	0.0	0.0	0.0	3.4168817831071143	0.0
64	0.0	0.0	0.0	3.613956199517029	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAATACC	20	0.006897733	52.500004	61
TAGCGTG	20	0.006897733	52.500004	4
TAGAAGA	20	0.006897733	52.500004	65
ATTGAGA	20	0.006897733	52.500004	59
AAAGCCC	20	0.006897733	52.500004	62
TACACAA	30	5.803426E-4	46.666664	5
ATCCTCC	35	0.0012396383	40.0	35
GCATTAT	35	0.0012396383	40.0	1
>>END_MODULE