FastQCFastQC Report
Wed 25 May 2016
SRR1781141_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781141_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50063
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAGAGCCCACTGTAAAGCTAACTTAGCATTAACCTTTTAAGTTAAAG660.13183388929948267No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGACA150.00221245870.04
GGGACAG308.264067E-658.3333325
GCGAGAT200.006913444552.562
CAGGTGC200.006913444552.557
GGGGACA200.006913444552.54
TAGAGCC200.006913444552.54
GCTGGAC200.006913444552.53
GTTAGCT200.006913444552.545
GCTTGTT200.006913444552.549
GATGGAA200.006913444552.521
TTAGTCA200.006913444552.537
TTGCATT200.006913444552.510
TATACTC200.006913444552.55
GTAAAGC200.006913444552.515
CTAGAGC305.825375E-446.6666643
GGATGGA350.001244298240.020
ATGAGTA400.002397466535.057
CATACAC501.6872656E-435.03
TGGCTCA400.002397466535.05
TGTAAAG400.002397466535.014
Produced by FastQC (version 0.11.5)