##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781141_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	50063
Sequences flagged as poor quality	0
Sequence length	76
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.71959331242634	34.0	34.0	34.0	31.0	34.0
2	32.97666939656033	34.0	34.0	34.0	32.0	34.0
3	32.961648323112875	34.0	34.0	34.0	32.0	34.0
4	32.994107424644945	34.0	34.0	34.0	33.0	34.0
5	33.05011685276551	34.0	34.0	34.0	33.0	34.0
6	36.43972594530891	38.0	37.0	38.0	34.0	38.0
7	36.368016299462674	38.0	37.0	38.0	34.0	38.0
8	36.3662385394403	38.0	37.0	38.0	34.0	38.0
9	36.43403311827098	38.0	37.0	38.0	34.0	38.0
10-11	36.427880870103664	38.0	37.0	38.0	34.0	38.0
12-13	36.37412859796656	38.0	37.0	38.0	34.0	38.0
14-15	36.34723248706629	38.0	37.0	38.0	34.0	38.0
16-17	36.34308770948604	38.0	37.0	38.0	34.0	38.0
18-19	36.350478397219504	38.0	37.0	38.0	34.0	38.0
20-21	36.294339132692805	38.0	37.0	38.0	34.0	38.0
22-23	36.343507180952	38.0	37.0	38.0	34.0	38.0
24-25	36.34669316661007	38.0	37.0	38.0	34.0	38.0
26-27	36.304516309450094	38.0	37.0	38.0	34.0	38.0
28-29	36.36402133312026	38.0	37.0	38.0	34.0	38.0
30-31	36.406567724666914	38.0	37.0	38.0	34.0	38.0
32-33	36.38772147094661	38.0	37.0	38.0	34.0	38.0
34-35	36.40871501907597	38.0	37.0	38.0	34.0	38.0
36-37	36.31573217745641	38.0	37.0	38.0	34.0	38.0
38-39	36.339622076184014	38.0	37.0	38.0	34.0	38.0
40-41	36.320256476839184	38.0	37.0	38.0	34.0	38.0
42-43	36.3921059465074	38.0	37.0	38.0	34.0	38.0
44-45	36.40078501088628	38.0	37.0	38.0	34.0	38.0
46-47	36.37011365679244	38.0	37.0	38.0	34.0	38.0
48-49	36.34820126640433	38.0	37.0	38.0	34.0	38.0
50-51	36.28542037033338	38.0	37.0	38.0	34.0	38.0
52-53	36.32360226115095	38.0	37.0	38.0	34.0	38.0
54-55	36.287867287218106	38.0	37.0	38.0	34.0	38.0
56-57	36.227733056349	38.0	37.0	38.0	34.0	38.0
58-59	36.23668178095599	38.0	37.0	38.0	34.0	38.0
60-61	36.242204821924375	38.0	37.0	38.0	34.0	38.0
62-63	36.20844735633102	38.0	37.0	38.0	34.0	38.0
64-65	36.20569082955476	38.0	37.0	38.0	34.0	38.0
66-67	36.157551485128735	38.0	37.0	38.0	34.0	38.0
68-69	35.8820685935721	38.0	37.0	38.0	32.0	38.0
70-71	36.11921978307333	38.0	37.0	38.0	34.0	38.0
72-73	36.13557917024549	38.0	37.0	38.0	34.0	38.0
74-75	36.155763737690506	38.0	37.0	38.0	34.0	38.0
76	35.160437848311126	38.0	36.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	8.0
14	42.0
15	61.0
16	100.0
17	129.0
18	147.0
19	155.0
20	122.0
21	117.0
22	125.0
23	142.0
24	153.0
25	200.0
26	189.0
27	241.0
28	324.0
29	410.0
30	550.0
31	677.0
32	999.0
33	1358.0
34	1925.0
35	3390.0
36	7447.0
37	31049.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.272956075345064	18.90018576593492	9.240357149991011	26.586501008729
2	22.703393723907876	20.803787228092606	31.448375047440223	25.044444000559295
3	18.239018836266304	22.273934842098956	30.431656113297244	29.055390208337496
4	12.676028204462378	17.106445878193476	37.74044703673371	32.477078880610435
5	11.966921678684857	34.410642590336174	37.26904100832951	16.353394722649462
6	27.357529512813855	39.32844615784112	19.089946667199328	14.224077662145696
7	27.103849150070914	31.272596528374248	22.63947426242934	18.984080059125503
8	24.15156902303098	35.379421928370256	20.23250704112818	20.236502007470587
9	23.654195713401112	16.187603619439507	21.211273795018275	38.946926872141105
10-11	23.31562231588199	25.371032499051193	28.62193636018617	22.69140882488065
12-13	23.885903761260813	23.738090006591694	27.169766094720654	25.206240137426843
14-15	22.064199109122505	24.849689391366876	24.699878153526555	28.386233345984056
16-17	22.059205401194497	27.95377823941833	25.716597087669534	24.270419271717635
18-19	21.26420709905519	28.34927990731678	26.8192077981743	23.567305195453727
20-21	21.164332940494976	28.039670015780118	27.233685556199188	23.562311487525715
22-23	21.500908854842898	27.59423126860156	27.33855342268741	23.566306453868126
24-25	21.136368176098117	27.73805005692827	27.78099594510916	23.34458582186445
26-27	20.939616083734496	27.664143179593715	27.48736591894213	23.90887481772966
28-29	21.2682020653976	27.782993428280367	28.240417074486146	22.708387431835884
30-31	21.447975550805985	27.941793340391108	27.42144897429239	23.188782134510515
32-33	20.81677086870543	28.010706509797657	27.642170864710465	23.530351756786448
34-35	21.6407326768272	27.359526995985057	27.79497832730759	23.204761999880148
36-37	21.79953258893794	27.49635459321255	27.17376106106306	23.530351756786448
38-39	21.19829015440545	28.24740826558536	27.688112977648167	22.866188602361028
40-41	21.51688872021253	26.929069372590536	28.014701476140065	23.539340431056868
42-43	21.202285120747856	27.567265245790306	27.83492799073168	23.39552164273016
44-45	20.81976709346224	27.414457783193175	27.769011046081935	23.99676407726265
46-47	21.27719073966802	27.0049737330963	27.768012304496338	23.949823222739347
48-49	20.765835047839722	27.442422547590034	28.085612128717813	23.706130275852427
50-51	21.05946507400675	27.22869184827118	27.83592673231728	23.87591634540479
52-53	21.078441164133192	26.918083215148915	27.963765655274354	24.03970996544354
54-55	20.41627549287897	27.46739108723009	28.39022831232647	23.726105107564468
56-57	20.323392525417976	27.347542096957834	28.194474961548448	24.134590416075742
58-59	20.6160238099994	27.251662904740027	28.16251523080918	23.969798054451392
60-61	20.164592613307235	27.194734634360707	28.238419591314944	24.402253161017118
62-63	19.647244471965326	27.16277490362144	29.059385174679903	24.130595449733335
64-65	20.53712322473683	27.20272456704552	28.16351397239478	24.096638235822862
66-67	20.268461738209854	26.904100832950483	28.206459860575674	24.62097756826399
68-69	20.044743623034975	26.471645726384757	28.149531590196354	25.334079060383917
70-71	20.472205021672693	26.892115933923254	28.015700217725666	24.619978826678384
72-73	20.58406407926013	26.425703613447055	27.9377983740487	25.05243393324411
74-75	20.707908035874798	26.47863691748397	27.769011046081935	25.044444000559295
76	20.502167269240758	26.452669636258314	28.238419591314944	24.806743503185984
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	1.0
12	2.0
13	3.0
14	3.0
15	4.0
16	6.5
17	7.0
18	12.5
19	26.5
20	44.5
21	54.0
22	69.5
23	106.0
24	153.0
25	179.0
26	231.0
27	306.0
28	411.5
29	494.0
30	545.5
31	645.0
32	891.5
33	1090.0
34	1185.5
35	1389.5
36	1695.0
37	1892.0
38	2049.0
39	2452.0
40	2929.5
41	3192.0
42	3223.0
43	3256.0
44	3311.0
45	3295.0
46	3257.0
47	3197.5
48	2893.5
49	2586.5
50	2524.0
51	2298.5
52	1880.5
53	1511.0
54	1334.0
55	1243.5
56	1074.0
57	883.5
58	772.0
59	654.0
60	485.0
61	392.5
62	351.0
63	306.5
64	248.5
65	237.5
66	230.0
67	220.0
68	218.5
69	214.0
70	201.5
71	192.0
72	181.5
73	169.0
74	172.0
75	177.0
76	141.0
77	102.5
78	88.0
79	76.0
80	67.0
81	55.0
82	42.0
83	32.0
84	29.5
85	19.5
86	9.5
87	7.0
88	5.5
89	3.5
90	2.5
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.5
98	0.5
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	50063.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.748716617062506
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.70580369082643	44.6357589437309
2	13.024872960684675	15.564388870023771
3	4.422974057234555	7.928010706509797
4	2.5006686279753945	5.976469648243214
5	1.497726664883659	4.474362303497593
6	1.0464027814923778	3.7512733955216424
7	0.7522064723188018	3.1460359946467453
8	0.4847552821610056	2.3170804785969676
9	0.3911473656057769	2.1033497792781097
>10	1.1700989569403584	9.971435990651779
>50	0.0033431398769724523	0.13183388929948267
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCTAGAGCCCACTGTAAAGCTAACTTAGCATTAACCTTTTAAGTTAAAG	66	0.13183388929948267	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0019974831712042825	0.0
2	0.0	0.0	0.0	0.0019974831712042825	0.0
3	0.0	0.0	0.0	0.0019974831712042825	0.0
4	0.0	0.0	0.0	0.0019974831712042825	0.0
5	0.0	0.0	0.0	0.0019974831712042825	0.0
6	0.0	0.0	0.0	0.0019974831712042825	0.0
7	0.0	0.0	0.0	0.0019974831712042825	0.0
8	0.0	0.0	0.0	0.0019974831712042825	0.0
9	0.0	0.0	0.0	0.0019974831712042825	0.0
10	0.0	0.0	0.0	0.0019974831712042825	0.0
11	0.0	0.0	0.0	0.0019974831712042825	0.0
12	0.0	0.0	0.0	0.0019974831712042825	0.0
13	0.0	0.0	0.0	0.0019974831712042825	0.0
14	0.0	0.0	0.0	0.0019974831712042825	0.0
15	0.0	0.0	0.0	0.0019974831712042825	0.0
16	0.0	0.0	0.0	0.0019974831712042825	0.0
17	0.0	0.0	0.0	0.0019974831712042825	0.0
18	0.0	0.0	0.0	0.0019974831712042825	0.0
19	0.0	0.0	0.0	0.0019974831712042825	0.0
20	0.0	0.0	0.0	0.0019974831712042825	0.0
21	0.0	0.0	0.0	0.0019974831712042825	0.0
22	0.0	0.0	0.0	0.003994966342408565	0.0
23	0.0	0.0	0.0	0.003994966342408565	0.0
24	0.0	0.0	0.0	0.005992449513612848	0.0
25	0.0	0.0	0.0	0.005992449513612848	0.0
26	0.0	0.0	0.0	0.005992449513612848	0.0
27	0.0	0.0	0.0	0.005992449513612848	0.0
28	0.0	0.0	0.0	0.005992449513612848	0.0
29	0.0	0.0	0.0	0.00798993268481713	0.0
30	0.0	0.0	0.0	0.011984899027225696	0.0
31	0.0	0.0	0.0	0.013982382198429979	0.0
32	0.0	0.0	0.0	0.017977348540838545	0.0
33	0.0	0.0	0.0	0.027964764396859958	0.0
34	0.0	0.0	0.0	0.03994966342408565	0.0
35	0.0	0.0	0.0	0.0699119109921499	0.0
36	0.0	0.0	0.0	0.10586660807382697	0.0
37	0.0	0.0	0.0	0.15180872101152548	0.0
38	0.0	0.0	0.0	0.23370553103090105	0.0
39	0.0	0.0	0.0	0.33957213910472805	0.0
40	0.0	0.0	0.0	0.4714060284042107	0.0
41	0.0	0.0	0.0	0.6172222999021233	0.0
42	0.0	0.0	0.0	0.7610410882288317	0.0
43	0.0	0.0	0.0	0.9807642370613028	0.0
44	0.0	0.0	0.0	1.2823841959131494	0.0
45	0.0	0.0	0.0	1.6099714359906518	0.0
46	0.0	0.0	0.0	2.0174580029163254	0.0
47	0.0	0.0	0.0	2.4269420530132035	0.0
48	0.0	0.0	0.0	2.848411002137307	0.0
49	0.0	0.0	0.0	3.3038371651718834	0.0
50	0.0	0.0	0.0	3.695343866727923	0.0
51	0.0	0.0	0.0	4.042905938517468	0.0
52	0.0	0.0	0.0	4.478357269840002	0.0
53	0.0	0.0	0.0	4.819926892115934	0.0
54	0.0	0.0	0.0	5.275353055150511	0.0
55	0.0	0.0	0.0	5.746759083554721	0.0
56	0.0	0.0	0.0	6.280087090266265	0.0
57	0.0	0.0	0.0	6.763478017697701	0.0
58	0.0	0.0	0.0	7.306793440265266	0.0
59	0.0	0.0	0.0	7.694305175478896	0.0
60	0.0	0.0	0.0	8.19567345145117	0.0
61	0.0	0.0	0.0	8.561212871781555	0.0
62	0.0	0.0	0.0	8.976689371392046	0.0
63	0.0	0.0	0.0	9.416135669056988	0.0
64	0.0	0.0	0.0	9.885544214289995	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGGACA	15	0.002212458	70.0	4
GGGACAG	30	8.264067E-6	58.333332	5
GCGAGAT	20	0.0069134445	52.5	62
CAGGTGC	20	0.0069134445	52.5	57
GGGGACA	20	0.0069134445	52.5	4
TAGAGCC	20	0.0069134445	52.5	4
GCTGGAC	20	0.0069134445	52.5	3
GTTAGCT	20	0.0069134445	52.5	45
GCTTGTT	20	0.0069134445	52.5	49
GATGGAA	20	0.0069134445	52.5	21
TTAGTCA	20	0.0069134445	52.5	37
TTGCATT	20	0.0069134445	52.5	10
TATACTC	20	0.0069134445	52.5	5
GTAAAGC	20	0.0069134445	52.5	15
CTAGAGC	30	5.825375E-4	46.666664	3
GGATGGA	35	0.0012442982	40.0	20
ATGAGTA	40	0.0023974665	35.0	57
CATACAC	50	1.6872656E-4	35.0	3
TGGCTCA	40	0.0023974665	35.0	5
TGTAAAG	40	0.0023974665	35.0	14
>>END_MODULE