FastQCFastQC Report
Wed 25 May 2016
SRR1781141_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781141_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48176
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT1450.3009797409498506No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATAA150.002211945870.035
CTCGAAT150.002211945870.033
GCAGCGT150.002211945870.01
CAGCGTC200.006911855752.52
AGCGTCA200.006911855752.53
GAATAAG200.006911855752.536
TCAGATG200.006911855752.57
TCGAATA200.006911855752.534
CGTATGC506.03668E-848.99999641
CGTCTTC506.03668E-848.99999648
TATGCCG506.03668E-848.99999643
CCGTCTT506.03668E-848.99999647
GTATGCC506.03668E-848.99999642
TCGTATG506.03668E-848.99999640
ATGCCGT506.03668E-848.99999644
GCCGTCT506.03668E-848.99999646
ATCTCGT451.6521371E-646.66666437
CTCGTAT451.6521371E-646.66666439
TCTCGTA451.6521371E-646.66666438
AATCTCG451.6521371E-646.66666436
Produced by FastQC (version 0.11.5)