##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781141_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	48176
Sequences flagged as poor quality	0
Sequence length	76
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.72291182331451	34.0	34.0	34.0	31.0	34.0
2	32.80693706409831	34.0	34.0	34.0	31.0	34.0
3	32.94335353703089	34.0	34.0	34.0	31.0	34.0
4	33.094694453669874	34.0	34.0	34.0	31.0	34.0
5	33.010544669545	34.0	34.0	34.0	31.0	34.0
6	35.98866655596147	38.0	36.0	38.0	32.0	38.0
7	35.87182414480239	38.0	36.0	38.0	32.0	38.0
8	35.4959315841913	37.0	36.0	38.0	31.0	38.0
9	35.5292261707074	37.0	36.0	38.0	31.0	38.0
10-11	35.46228412487545	37.0	35.5	38.0	31.0	38.0
12-13	35.386883510461644	37.0	35.0	38.0	31.0	38.0
14-15	35.44489995018266	37.0	36.0	38.0	31.0	38.0
16-17	35.37870516439721	37.0	35.5	38.0	31.0	38.0
18-19	35.40520176021255	37.0	35.5	38.0	31.0	38.0
20-21	35.286916722019264	37.0	35.0	38.0	30.5	38.0
22-23	35.348607190302225	37.0	35.0	38.0	31.0	38.0
24-25	35.36991448023912	37.0	35.0	38.0	31.0	38.0
26-27	35.03685445034872	37.0	35.0	38.0	27.0	38.0
28-29	34.95308867485885	37.0	35.0	38.0	27.0	38.0
30-31	35.09353204915311	37.0	35.0	38.0	27.0	38.0
32-33	34.951002573895714	37.0	35.0	38.0	27.0	38.0
34-35	34.985200099634675	37.0	35.0	38.0	27.0	38.0
36-37	34.93098223181667	37.0	35.0	38.0	27.0	38.0
38-39	34.964328711391566	37.0	35.0	38.0	27.0	38.0
40-41	34.949508053802724	37.0	35.0	38.0	27.0	38.0
42-43	34.90101917967452	37.0	35.0	38.0	27.0	38.0
44-45	34.84125913317835	37.0	34.0	38.0	27.0	38.0
46-47	34.91581908003985	37.0	35.0	38.0	27.0	38.0
48-49	34.882036698771174	37.0	34.5	38.0	27.0	38.0
50-51	34.78502781467951	37.0	34.0	38.0	27.0	38.0
52-53	34.774618067087346	37.0	34.0	38.0	27.0	38.0
54-55	34.778416638990365	37.0	34.0	38.0	27.0	38.0
56-57	34.74429176353371	37.0	34.0	38.0	27.0	38.0
58-59	34.727187811358355	37.0	34.0	38.0	27.0	38.0
60-61	34.71989164729325	37.0	34.0	38.0	27.0	38.0
62-63	34.68291265360345	37.0	34.0	38.0	27.0	38.0
64-65	34.65069744271007	37.0	34.0	38.0	27.0	38.0
66-67	34.6204022749917	37.0	34.0	38.0	27.0	38.0
68-69	34.51057580538027	37.0	34.0	38.0	26.5	38.0
70-71	34.54098513782796	37.0	34.0	38.0	27.0	38.0
72-73	34.542126785121226	37.0	34.0	38.0	27.0	38.0
74-75	34.520809116572565	37.0	34.0	38.0	26.5	38.0
76	33.49931501162405	37.0	34.0	38.0	24.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	2.0
21	7.0
22	16.0
23	23.0
24	77.0
25	138.0
26	276.0
27	458.0
28	781.0
29	1132.0
30	1815.0
31	2361.0
32	3272.0
33	4409.0
34	5607.0
35	7171.0
36	9138.0
37	11493.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.689721022915975	19.588591829956826	9.367734971770176	23.353952175357023
2	25.022832945865158	22.56517768183328	30.185154433742944	22.226834938558618
3	20.989704417137165	24.389737628694785	29.987960810362008	24.632597143806045
4	14.880853537030886	18.478080371969448	38.19536698771173	28.445699103287946
5	13.502573895715708	36.72367984058452	35.52391232148788	14.24983394221189
6	29.50224178013949	39.25606110926603	19.24402191962803	11.997675190966456
7	29.159747592162073	31.303968781135836	22.702175357024245	16.83410826967785
8	25.799153105280638	36.36665559614746	19.33950514779143	18.49468615078047
9	25.257389571570904	17.058286283626703	21.616572567253403	36.06775157754898
10-11	25.005189305878446	25.661117568913983	28.732148787778144	20.601544337429427
12-13	25.318623380936568	24.101212221853206	27.26668880770508	23.31347558950515
14-15	23.30932414480239	24.909706077715043	24.95122052474261	26.829749252739955
16-17	23.797118897376286	28.52769013616739	25.691215543008965	21.98397542344736
18-19	23.04570740617735	27.966207240119562	27.145259050149452	21.842826303553636
20-21	22.73331119229492	28.11358352706742	27.222060777150446	21.93104450348721
22-23	23.279226170707407	27.52407837927599	27.448314513450683	21.748380936565926
24-25	23.158834274327468	27.81467950846895	27.356982729990037	21.66950348721355
26-27	22.558950514779145	28.04404682829625	27.625788774493525	21.771213882431088
28-29	22.602540684158086	27.362172035868483	28.650157754898704	21.385129525074724
30-31	22.846438060445035	27.73476419794088	27.988002324809035	21.430795416805047
32-33	22.36902191962803	28.24435403520425	27.605031550979742	21.781592494187976
34-35	23.30621056127532	27.16082696778479	28.404184656260377	21.12877781467951
36-37	23.451511125871804	27.912238458983722	27.05185154433743	21.584398870807043
38-39	22.619146462969113	28.194536698771174	28.00357024244437	21.182746595815345
40-41	22.777939222849554	26.89098306210561	28.534955164397207	21.796122550647627
42-43	22.972019262703423	27.503321155762205	27.877989040185984	21.64667054134839
44-45	22.42818000664231	27.904973430753905	27.77524078379276	21.891605778811027
46-47	23.14534207904351	27.149410494852212	27.515775489870475	22.189471936233808
48-49	22.763409166389902	26.852582198605113	28.059614745931583	22.324393889073395
50-51	22.581783460644303	26.826635669212884	28.164438724676188	22.42714214546662
52-53	22.207115576220524	26.763326137495845	29.016522749916973	22.013035536366658
54-55	22.010959814015276	26.770591165725673	28.683369312520757	22.53507970773829
56-57	22.25900863500498	26.572359681169043	28.55882597143806	22.609805712387914
58-59	22.024452009299235	26.53084523414148	28.89509299236134	22.549609764197942
60-61	21.834523414148123	26.913816007970777	28.438434075058122	22.813226502822985
62-63	20.881766854865493	27.212720026569247	29.012371305214213	22.893141813351043
64-65	21.38824310860179	27.569744271006307	27.95479076718698	23.087221853204916
66-67	21.562603786117567	27.088176685486548	27.80222517436068	23.546994354035206
68-69	21.106982729990037	27.73891564264364	27.610220856858188	23.54388077050814
70-71	21.419378943872466	27.319619727665227	27.61956160743939	23.641439721022916
72-73	21.570906675523084	26.44989206243773	27.714006974427104	24.265194287612086
74-75	21.350880106276986	26.877490866821653	27.408875788774495	24.36275323812687
76	21.51486217203587	26.25788774493524	28.09905347060777	24.128196612421124
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	0.5
8	0.0
9	0.0
10	0.5
11	0.5
12	0.0
13	1.0
14	1.5
15	5.0
16	11.5
17	14.0
18	19.5
19	28.5
20	44.5
21	57.0
22	66.0
23	109.0
24	165.5
25	188.0
26	235.0
27	315.0
28	421.5
29	495.0
30	529.5
31	664.0
32	912.5
33	1061.0
34	1153.0
35	1376.0
36	1642.5
37	1778.0
38	1941.0
39	2376.0
40	2835.0
41	3069.0
42	3116.0
43	3180.5
44	3232.5
45	3179.0
46	3138.0
47	3012.5
48	2744.0
49	2471.0
50	2341.0
51	2163.5
52	1810.0
53	1489.5
54	1345.0
55	1261.0
56	1029.0
57	773.5
58	666.0
59	565.5
60	438.5
61	355.5
62	299.0
63	288.5
64	257.0
65	232.5
66	216.0
67	203.0
68	208.0
69	203.5
70	186.0
71	178.0
72	157.0
73	144.0
74	138.5
75	125.0
76	118.5
77	106.5
78	87.0
79	73.0
80	59.5
81	43.5
82	34.0
83	27.0
84	28.0
85	20.0
86	7.0
87	3.0
88	3.5
89	2.5
90	1.0
91	0.5
92	0.0
93	2.0
94	2.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	48176.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.65410162736632
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.39516076436445	49.26519428761209
2	11.765473162460054	14.978412487545667
3	4.138133437683428	7.90227499169711
4	2.197873866823192	5.5961474593158425
5	1.4609013239418247	4.649618067087347
6	0.8478445183590947	3.2381268681501165
7	0.5967521033065937	2.659000332115576
8	0.42066131872432005	2.1421454666223845
9	0.28370181960477403	1.625290601129193
>10	0.8902367442770496	7.642809697774826
>50	0.0	0.0
>100	0.0032609404552272877	0.3009797409498506
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT	145	0.3009797409498506	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0020757223513782797	0.0
2	0.0	0.0	0.0	0.0020757223513782797	0.0
3	0.0	0.0	0.0	0.0020757223513782797	0.0
4	0.0	0.0	0.0	0.0020757223513782797	0.0
5	0.0	0.0	0.0	0.0020757223513782797	0.0
6	0.0	0.0	0.0	0.0020757223513782797	0.0
7	0.0	0.0	0.0	0.0020757223513782797	0.0
8	0.0	0.0	0.0	0.0020757223513782797	0.0
9	0.0	0.0	0.0	0.0020757223513782797	0.0
10	0.0	0.0	0.0	0.0020757223513782797	0.0
11	0.0	0.0	0.0	0.0020757223513782797	0.0
12	0.0	0.0	0.0	0.0020757223513782797	0.0
13	0.0	0.0	0.0	0.0020757223513782797	0.0
14	0.0	0.0	0.0	0.0020757223513782797	0.0
15	0.0	0.0	0.0	0.0020757223513782797	0.0
16	0.0	0.0	0.0	0.0020757223513782797	0.0
17	0.0	0.0	0.0	0.0020757223513782797	0.0
18	0.0	0.0	0.0	0.0020757223513782797	0.0
19	0.0	0.0	0.0	0.0020757223513782797	0.0
20	0.0	0.0	0.0	0.0020757223513782797	0.0
21	0.0	0.0	0.0	0.0020757223513782797	0.0
22	0.0	0.0	0.0	0.004151444702756559	0.0
23	0.0	0.0	0.0	0.004151444702756559	0.0
24	0.0	0.0	0.0	0.004151444702756559	0.0
25	0.0	0.0	0.0	0.004151444702756559	0.0
26	0.0	0.0	0.0	0.004151444702756559	0.0
27	0.0	0.0	0.0	0.004151444702756559	0.0
28	0.0	0.0	0.0	0.004151444702756559	0.0
29	0.0	0.0	0.0	0.006227167054134839	0.0
30	0.0	0.0	0.0	0.010378611756891398	0.0
31	0.0	0.0	0.0	0.012454334108269677	0.0
32	0.0	0.0	0.0	0.016605778811026237	0.0
33	0.0	0.0	0.0	0.026984390567917637	0.0
34	0.0	0.0	0.0	0.03736300232480903	0.0
35	0.0	0.0	0.0	0.06227167054134839	0.0
36	0.0	0.0	0.0	0.09340750581202259	0.0
37	0.0	0.0	0.0	0.1432248422451013	0.0
38	0.0	0.0	0.0	0.21795084689471936	0.0
39	0.0	0.0	0.0	0.31966124211225505	0.0
40	0.0	0.0	0.0	0.44628030554633014	0.0
41	0.0	0.0	0.0	0.5832779807372965	0.0
42	0.0	0.0	0.0	0.7119727665227499	0.0
43	0.0	0.0	0.0	0.927847891066091	0.0
44	0.0	0.0	0.0	1.2059946861507804	0.0
45	0.0	0.0	0.0	1.5256559282630355	0.0
46	0.0	0.0	0.0	1.9117402856193955	0.0
47	0.0	0.0	0.0	2.3040518100298906	0.0
48	0.0	0.0	0.0	2.692211889737629	0.0
49	0.0	0.0	0.0	3.130189305878446	0.0
50	0.0	0.0	0.0	3.4975921620724013	0.0
51	0.0	0.0	0.0	3.8193291265360347	0.0
52	0.0	0.0	0.0	4.24692793091996	0.0
53	0.0	0.0	0.0	4.570740617734971	0.0
54	0.0	0.0	0.0	5.017020923281302	0.0
55	0.0	0.0	0.0	5.4799070076386585	0.0
56	0.0	0.0	0.0	5.986383261374958	0.0
57	0.0	0.0	0.0	6.463799402191963	0.0
58	0.0	0.0	0.0	6.978578545333776	0.0
59	0.0	0.0	0.0	7.383344403852541	0.0
60	0.0	0.0	0.0	7.858684822318167	0.0
61	0.0	0.0	0.0	8.197027565592826	0.0
62	0.0	0.0	0.0	8.610096313517104	0.0
63	0.0	0.0	0.0	9.031467950846896	0.0
64	0.0	0.0	0.0	9.490202590501495	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATAA	15	0.0022119458	70.0	35
CTCGAAT	15	0.0022119458	70.0	33
GCAGCGT	15	0.0022119458	70.0	1
CAGCGTC	20	0.0069118557	52.5	2
AGCGTCA	20	0.0069118557	52.5	3
GAATAAG	20	0.0069118557	52.5	36
TCAGATG	20	0.0069118557	52.5	7
TCGAATA	20	0.0069118557	52.5	34
CGTATGC	50	6.03668E-8	48.999996	41
CGTCTTC	50	6.03668E-8	48.999996	48
TATGCCG	50	6.03668E-8	48.999996	43
CCGTCTT	50	6.03668E-8	48.999996	47
GTATGCC	50	6.03668E-8	48.999996	42
TCGTATG	50	6.03668E-8	48.999996	40
ATGCCGT	50	6.03668E-8	48.999996	44
GCCGTCT	50	6.03668E-8	48.999996	46
ATCTCGT	45	1.6521371E-6	46.666664	37
CTCGTAT	45	1.6521371E-6	46.666664	39
TCTCGTA	45	1.6521371E-6	46.666664	38
AATCTCG	45	1.6521371E-6	46.666664	36
>>END_MODULE