FastQCFastQC Report
Wed 25 May 2016
SRR1781140_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781140_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111387
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTGC150.002219616770.0000155
TAGAAAG200.00693564352.563
TAAGTCA200.00693564352.54
TTATCGG200.00693564352.535
ATTCAGG200.00693564352.521
AACGCAC200.00693564352.532
GGTGCCC200.00693564352.557
ATCGGTG200.00693564352.554
TATCGGA200.00693564352.536
CGATATG200.00693564352.565
AATCGGT200.00693564352.553
TAACTCC200.00693564352.558
ATTAGAG305.856431E-446.6666683
AAAGTCT305.856431E-446.66666850
AGCCTTA305.856431E-446.6666686
AACCGAT350.001250891640.00000433
TCGTTGT350.001250891640.00000434
CCGATAC350.001250891640.00000444
ACCGATA350.001250891640.00000434
GGATAAA350.001250891640.0000046