FastQCFastQC Report
Wed 25 May 2016
SRR1781140_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781140_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104798
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT8420.8034504475276245No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGCAT150.00221924870.0000162
CGGGCTA150.00221924870.0000141
ATGTATC207.8950776E-570.016
CTCGTAT1100.060.45454439
TCGTATG1150.057.82608440
TCTCGTA850.057.6470638
TGTATCC252.3809842E-455.99999617
TACAGGA252.3809842E-455.9999967
CGTATGC1200.055.4166741
GCCGTCT1200.055.4166746
ATCTCGT900.054.44444737
TATGCCG1250.053.20000543
CCGTCTT1200.052.50000447
GGTCGGG200.00693449952.554
TAGCGAG200.00693449952.528
GAATCGT200.00693449952.525
CCCAACG200.00693449952.521
CTACGGG200.00693449952.538
AAATTCG200.00693449952.562
CCAACGT200.00693449952.522
Produced by FastQC (version 0.11.5)