##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781140_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	104798
Sequences flagged as poor quality	0
Sequence length	76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.02939941601939	34.0	34.0	34.0	31.0	34.0
2	33.05420904979103	34.0	34.0	34.0	31.0	34.0
3	33.11856142292792	34.0	34.0	34.0	31.0	34.0
4	33.221359186244015	34.0	34.0	34.0	32.0	34.0
5	33.19337201091624	34.0	34.0	34.0	32.0	34.0
6	36.40341418729365	38.0	37.0	38.0	34.0	38.0
7	36.29147502814939	38.0	37.0	38.0	34.0	38.0
8	36.02706158514476	38.0	36.0	38.0	32.0	38.0
9	36.00696578178973	38.0	36.0	38.0	32.0	38.0
10-11	35.938758373251396	38.0	36.0	38.0	32.0	38.0
12-13	35.87108055497242	38.0	36.0	38.0	31.0	38.0
14-15	35.8655508692914	38.0	36.0	38.0	31.0	38.0
16-17	35.795139220214125	38.0	36.0	38.0	31.0	38.0
18-19	35.80271570068131	38.0	36.0	38.0	31.0	38.0
20-21	35.73190328059696	38.0	36.0	38.0	31.0	38.0
22-23	35.80892765129106	38.0	36.0	38.0	31.0	38.0
24-25	35.78979083570297	38.0	36.0	38.0	31.0	38.0
26-27	35.517342888223055	38.0	36.0	38.0	31.0	38.0
28-29	35.50681787820378	38.0	36.0	38.0	31.0	38.0
30-31	35.52970476535812	38.0	36.0	38.0	31.0	38.0
32-33	35.45771388766961	38.0	36.0	38.0	31.0	38.0
34-35	35.46918834328899	38.0	36.0	38.0	31.0	38.0
36-37	35.44166396305273	38.0	36.0	38.0	31.0	38.0
38-39	35.39456382755396	38.0	36.0	38.0	31.0	38.0
40-41	35.41829519647321	38.0	36.0	38.0	31.0	38.0
42-43	35.41675413652932	38.0	36.0	38.0	31.0	38.0
44-45	35.4049695604878	38.0	36.0	38.0	31.0	38.0
46-47	35.40357640412985	38.0	36.0	38.0	31.0	38.0
48-49	35.39474512872383	38.0	36.0	38.0	31.0	38.0
50-51	35.305955266321874	38.0	36.0	38.0	31.0	38.0
52-53	35.33996832000611	38.0	36.0	38.0	31.0	38.0
54-55	35.35396667875341	38.0	36.0	38.0	31.0	38.0
56-57	35.27420847726101	38.0	36.0	38.0	29.5	38.0
58-59	35.26991450218516	38.0	36.0	38.0	29.5	38.0
60-61	35.28606461955381	38.0	36.0	38.0	30.0	38.0
62-63	35.247394988453976	38.0	36.0	38.0	28.5	38.0
64-65	35.17180671386859	38.0	35.5	38.0	27.0	38.0
66-67	35.17067596709861	37.5	35.5	38.0	27.5	38.0
68-69	35.07186205843623	37.0	35.0	38.0	27.0	38.0
70-71	35.01880283974885	37.5	35.0	38.0	27.0	38.0
72-73	34.954493406362715	37.5	35.0	38.0	27.0	38.0
74-75	34.9651567778011	38.0	35.0	38.0	27.0	38.0
76	33.94017061394302	37.0	34.0	38.0	24.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	4.0
21	5.0
22	25.0
23	34.0
24	101.0
25	210.0
26	419.0
27	732.0
28	1244.0
29	1978.0
30	3036.0
31	4249.0
32	5878.0
33	7995.0
34	10447.0
35	13831.0
36	19811.0
37	34797.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.80619859157618	19.454569743697398	10.197713696826275	25.541517967900152
2	23.60159545029485	21.69697894997996	30.93379644649707	23.767629153228114
3	20.1129792553293	23.37162922956545	29.811637626672265	26.703753888432985
4	14.147216549934157	18.25607358919063	37.667703582129434	29.929006278745778
5	13.618580507261587	35.25639802286303	36.32798335846104	14.79703811141434
6	29.272505200480925	38.99692742227905	18.356266340960705	13.374301036279318
7	28.371724651233805	30.518712189163917	22.25710414320884	18.852459016393443
8	25.607358919063337	35.770720815282736	19.263726406992497	19.358193858661423
9	24.57298803412279	16.487910074619744	21.41453081165671	37.524571079600754
10-11	24.85352773907899	25.7266360045039	27.799194641119108	21.620641615298002
12-13	24.965171091051356	23.26141720261837	26.881238191568542	24.89217351476173
14-15	22.920284738258363	24.50953262466841	23.88118093856753	28.689001698505695
16-17	23.87688696349167	27.929445218420202	24.94656386572263	23.247103952365503
18-19	23.147865417278958	27.14126223782897	26.389339491211665	23.321532853680413
20-21	22.62495467470753	27.200423672207485	26.20612988797496	23.96849176511002
22-23	23.251875035783126	27.06540201148877	26.375980457642324	23.306742495085782
24-25	23.30006297830111	27.40033206740587	26.54821656901849	22.75138838527453
26-27	22.558159506860818	27.775339224030994	26.73285749728048	22.933643771827704
28-29	23.466096681234376	26.55775873585374	27.372182675241895	22.603961907669994
30-31	23.58775930838375	26.393156357945763	27.37504532529247	22.644039008378023
32-33	22.561499265253154	27.81684764976431	26.24143590526537	23.38021717971717
34-35	23.184125651252888	26.478081642779443	27.986221111089904	22.351571594877765
36-37	23.266188286035995	26.715681596977042	26.945647817706448	23.07248229928052
38-39	22.375427011965876	27.221416439245022	27.549666978377452	22.85348957041165
40-41	22.453672780014884	26.167961220633984	27.96570545239413	23.412660546957003
42-43	23.251875035783126	26.25908891391057	27.40701158419054	23.082024466115765
44-45	22.031431897555297	26.968549018111034	27.624095879692362	23.37592320464131
46-47	22.86160041222161	25.812038397679345	27.35214412488788	23.974217065211167
48-49	22.5672245653543	25.3349300559171	27.89175365942098	24.206091719307622
50-51	21.808145193610564	25.5978167522281	28.173247581060707	24.42079047310063
52-53	21.778564476421305	25.14647226092101	29.263917250329204	23.81104601232848
54-55	20.977499570602493	25.748582988224967	29.025840187789843	24.2480772533827
56-57	21.16834290730739	25.849729956678562	28.204736731617015	24.777190404397032
58-59	20.995629687589457	25.653161319872513	28.76199927479532	24.589209717742705
60-61	21.026164621462243	25.765758888528406	28.35598007595565	24.852096414053705
62-63	19.64779862211111	26.553464760777878	28.543483654268208	25.2552529628428
64-65	20.200767190213554	26.86119964121453	27.512929636061756	25.425103532510164
66-67	20.311456325502395	26.446592492223136	26.91654420885895	26.32540697341552
68-69	20.123952747189833	27.019599610679595	26.827802057291173	26.028645584839406
70-71	20.58865627206626	26.394110574629288	26.639344262295083	26.37788889100937
72-73	20.706502032481534	25.55010591805187	26.301551556327414	27.441840493139182
74-75	20.70745624916506	25.63026011946793	26.58113704460009	27.081146586766923
76	21.127311589915838	25.64361915303727	26.343059982060723	26.88600927498616
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.5
11	1.0
12	0.0
13	0.0
14	1.5
15	5.5
16	16.5
17	25.0
18	25.0
19	41.5
20	68.5
21	79.0
22	100.0
23	163.0
24	275.0
25	345.0
26	403.5
27	496.0
28	636.5
29	743.0
30	922.5
31	1171.5
32	1616.0
33	1991.0
34	2152.0
35	2576.0
36	3236.0
37	3633.0
38	3905.0
39	4781.5
40	5890.5
41	6585.5
42	6776.0
43	6879.5
44	6999.0
45	6952.0
46	6889.0
47	6689.5
48	6144.5
49	5497.0
50	5195.0
51	4816.5
52	3973.0
53	3227.5
54	2947.0
55	2689.0
56	2145.5
57	1660.0
58	1460.0
59	1254.0
60	967.5
61	829.0
62	771.0
63	701.5
64	679.5
65	695.5
66	687.0
67	710.0
68	738.5
69	750.5
70	733.0
71	732.0
72	695.5
73	638.5
74	587.5
75	557.0
76	532.5
77	444.5
78	330.5
79	280.0
80	248.5
81	195.0
82	144.5
83	116.0
84	88.0
85	49.5
86	27.0
87	15.0
88	13.0
89	11.5
90	6.5
91	2.0
92	3.0
93	1.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	104798.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.50762419130136
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.04381011121177	34.18195003721445
2	15.211148160283717	15.061356132750625
3	5.265693965268007	7.820759938166759
4	2.756201453269857	5.458119429760109
5	1.7365996569203785	4.298746159277849
6	1.2412543607732784	3.6870932651386474
7	0.9213037025615326	3.1928090230729596
8	0.7073607925524739	2.8015801828279163
9	0.5319661546171193	2.370274241874845
>10	2.567314920108706	19.824805816904902
>50	0.015419308829481719	0.4990553254833108
>100	0.0	0.0
>500	0.0019274136036852149	0.8034504475276245
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT	842	0.8034504475276245	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	9.542166835244947E-4	0.0
20	0.0	0.0	0.0	9.542166835244947E-4	0.0
21	0.0	0.0	0.0	9.542166835244947E-4	0.0
22	0.0	0.0	0.0	0.0019084333670489895	0.0
23	0.0	0.0	0.0	0.0019084333670489895	0.0
24	0.0	0.0	0.0	0.002862650050573484	0.0
25	0.0	0.0	0.0	0.002862650050573484	0.0
26	0.0	0.0	0.0	0.003816866734097979	0.0
27	0.0	0.0	0.0	0.009542166835244947	0.0
28	0.0	0.0	0.0	0.010496383518769442	0.0
29	0.0	0.0	0.0	0.0181301169869654	0.0
30	0.0	0.0	0.0	0.027672283822210347	0.0
31	0.0	0.0	0.0	0.03435180060688181	0.0
32	0.0	0.0	0.0	0.04771083417622474	0.0
33	0.0	0.0	0.0	0.0734746846313861	0.0
34	0.0	0.0	0.0	0.10210118513712094	0.0
35	0.0	0.0	0.0	0.16889635298383557	0.0
36	0.0	0.0	0.0	0.26336380465276055	0.0
37	0.0	0.0	0.0	0.43035172426954715	0.0
38	0.0	0.0	0.0	0.626920361075593	0.0
39	0.0	0.0	0.0	0.8673829653237657	0.0
40	0.0	0.0	0.0	1.1202503864577569	0.0
41	0.0	0.0	0.0	1.4103322582492033	0.0
42	0.0	0.0	0.0	1.7672092978873644	0.0
43	0.0	0.0	0.0	2.216645355827401	0.0
44	0.0	0.0	0.0	2.774862115689231	0.0
45	0.0	0.0	0.0	3.495295711750224	0.0
46	0.0	0.0	0.0	4.245310025000477	0.0
47	0.0	0.0	0.0	5.0525773392622	0.0
48	0.0	0.0	0.0	5.76823985190557	0.0
49	0.0	0.0	0.0	6.504895131586481	0.0
50	0.0	0.0	0.0	7.217694994179278	0.0
51	0.0	0.0	0.0	7.895188839481669	0.0
52	0.0	0.0	0.0	8.554552567797096	0.0
53	0.0	0.0	0.0	9.231092196415963	0.0
54	0.0	0.0	0.0	9.978243859615642	0.0
55	0.0	0.0	0.0	10.778831657092693	0.0
56	0.0	0.0	0.0	11.59564113818966	0.0
57	0.0	0.0	0.0	12.405771102501957	0.0
58	0.0	0.0	0.0	13.207313116662531	0.0
59	0.0	0.0	0.0	13.974503330216226	0.0
60	0.0	0.0	0.0	14.735968243668772	0.0
61	0.0	0.0	0.0	15.46594400656501	0.0
62	0.0	0.0	0.0	16.181606519208383	0.0
63	0.0	0.0	0.0	16.905856982003474	0.0
64	0.0	0.0	0.0	17.632015878165614	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGCAT	15	0.002219248	70.00001	62
CGGGCTA	15	0.002219248	70.00001	41
ATGTATC	20	7.8950776E-5	70.0	16
CTCGTAT	110	0.0	60.454544	39
TCGTATG	115	0.0	57.826084	40
TCTCGTA	85	0.0	57.64706	38
TGTATCC	25	2.3809842E-4	55.999996	17
TACAGGA	25	2.3809842E-4	55.999996	7
CGTATGC	120	0.0	55.41667	41
GCCGTCT	120	0.0	55.41667	46
ATCTCGT	90	0.0	54.444447	37
TATGCCG	125	0.0	53.200005	43
CCGTCTT	120	0.0	52.500004	47
GGTCGGG	20	0.006934499	52.5	54
TAGCGAG	20	0.006934499	52.5	28
GAATCGT	20	0.006934499	52.5	25
CCCAACG	20	0.006934499	52.5	21
CTACGGG	20	0.006934499	52.5	38
AAATTCG	20	0.006934499	52.5	62
CCAACGT	20	0.006934499	52.5	22
>>END_MODULE