FastQCFastQC Report
Wed 25 May 2016
SRR1781139_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781139_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41994
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGGG150.00220995170.011
TTGTCTA150.00220995170.020
AGAACTG150.00220995170.037
GTCCCAA252.3644877E-455.99999651
TTAATGT200.006905668452.5000045
CCAGGAT200.006905668452.5000043
GAGAACT200.006905668452.50000436
TTTTCAC200.006905668452.50000447
TATATCC400.00239304835.0000045
CCACCAA450.004261760531.11110947
AATATTA500.007132687627.99999853
TATTATA850.003731109220.5882362
Produced by FastQC (version 0.11.5)