##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781139_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	41994
Sequences flagged as poor quality	0
Sequence length	76
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.27311044434919	34.0	34.0	34.0	30.0	34.0
2	32.39693765776063	34.0	34.0	34.0	31.0	34.0
3	32.41884554936419	34.0	34.0	34.0	31.0	34.0
4	32.47506786683812	34.0	34.0	34.0	31.0	34.0
5	32.517669190836784	34.0	34.0	34.0	31.0	34.0
6	35.615683192837075	38.0	37.0	38.0	31.0	38.0
7	35.57298661713578	38.0	37.0	38.0	31.0	38.0
8	35.53621945992285	38.0	37.0	38.0	31.0	38.0
9	35.663737676810975	38.0	37.0	38.0	31.0	38.0
10-11	35.577130066199935	38.0	37.0	38.0	31.0	38.0
12-13	35.54113682907082	38.0	37.0	38.0	31.0	38.0
14-15	35.506108015430776	38.0	37.0	38.0	31.0	38.0
16-17	35.50627470591037	38.0	37.0	38.0	31.0	38.0
18-19	35.51170405295995	38.0	37.0	38.0	31.0	38.0
20-21	35.479211315902276	38.0	37.0	38.0	31.0	38.0
22-23	35.49026051340668	38.0	37.0	38.0	31.0	38.0
24-25	35.48273562889936	38.0	37.0	38.0	31.0	38.0
26-27	35.441777396770966	38.0	36.5	38.0	31.0	38.0
28-29	35.482461780254326	38.0	37.0	38.0	31.0	38.0
30-31	35.53868409772825	38.0	37.0	38.0	31.0	38.0
32-33	35.52507501071582	38.0	37.0	38.0	31.0	38.0
34-35	35.562425584607325	38.0	37.0	38.0	31.0	38.0
36-37	35.427061008715526	38.0	37.0	38.0	31.0	38.0
38-39	35.44168214506834	38.0	37.0	38.0	31.0	38.0
40-41	35.471055388865075	38.0	37.0	38.0	31.0	38.0
42-43	35.5076915749869	38.0	37.0	38.0	31.0	38.0
44-45	35.56283040434347	38.0	37.0	38.0	31.0	38.0
46-47	35.520919655188834	38.0	37.0	38.0	31.0	38.0
48-49	35.47453207601086	38.0	37.0	38.0	31.0	38.0
50-51	35.39418726484736	38.0	36.5	38.0	30.5	38.0
52-53	35.45927989712816	38.0	37.0	38.0	31.0	38.0
54-55	35.44412296994808	38.0	37.0	38.0	31.0	38.0
56-57	35.37920893460971	38.0	36.5	38.0	29.5	38.0
58-59	35.37564890222413	38.0	36.5	38.0	31.0	38.0
60-61	35.370374339191315	38.0	36.5	38.0	30.0	38.0
62-63	35.357539172262705	38.0	36.5	38.0	30.0	38.0
64-65	35.370052864694955	38.0	36.0	38.0	30.5	38.0
66-67	35.27165785588417	38.0	36.0	38.0	27.0	38.0
68-69	34.97632995189789	38.0	36.0	38.0	26.5	38.0
70-71	35.206720007620135	38.0	36.0	38.0	27.0	38.0
72-73	35.22728246892413	38.0	36.0	38.0	27.5	38.0
74-75	35.24261799304662	38.0	36.0	38.0	27.0	38.0
76	34.27644425394104	37.0	34.0	38.0	25.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	5.0
13	17.0
14	58.0
15	99.0
16	164.0
17	199.0
18	237.0
19	190.0
20	164.0
21	173.0
22	147.0
23	178.0
24	201.0
25	243.0
26	292.0
27	366.0
28	497.0
29	551.0
30	704.0
31	962.0
32	1218.0
33	1655.0
34	2448.0
35	3713.0
36	7293.0
37	20219.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.856408058294036	19.478973186645714	9.175120255274564	26.489498499785686
2	21.422107920179073	20.84583511930276	33.47621088726961	24.25584607324856
3	18.47644901652617	22.45320760108587	30.644854026765728	28.42548935562223
4	13.313806734295374	16.38567414392532	37.33628613611468	32.96423298566462
5	12.828023050912035	33.464304424441586	37.005286469495644	16.702386055150736
6	29.032718959851405	38.086393294280136	18.493118064485405	14.387769681383055
7	26.727627756346145	30.432918988426916	23.058055912749438	19.781397342477497
8	23.572415106920037	36.826689527075295	20.571986474258228	19.02890889174644
9	23.050912035052626	16.283278563604327	22.21507834452541	38.45073105681764
10-11	23.592656093727676	25.42863266180883	28.536219459922847	22.44249178454065
12-13	23.349764252036003	23.684335857503452	28.850550078582653	24.115349811877888
14-15	21.376863361432584	24.791636900509598	25.447683002333665	28.38381673572415
16-17	21.57570129066057	28.676715721293515	26.070391008239273	23.67719197980664
18-19	21.283992951374007	28.194503976758583	27.44439681859313	23.077106253274277
20-21	21.50188122112683	27.90279563747202	27.393199028432637	23.20212411296852
22-23	21.36257560603896	27.54083916750012	27.759918083535744	23.33666714292518
24-25	21.600704862599418	27.316997666333286	27.42296518550269	23.659332285564606
26-27	20.948230699623753	27.987331523550985	27.869457541553555	23.194980235271707
28-29	21.11015859408487	27.288422155546034	28.601705005476973	22.99971424489213
30-31	21.344715911796925	27.744439681859312	27.955184073915323	22.955660332428444
32-33	20.772015049769017	27.550364337762538	28.256417583464305	23.421203029004143
34-35	21.566176120398154	27.062199361813594	27.977806353288564	23.39381816449969
36-37	21.257798733152356	27.782540362908986	27.66109444206315	23.298566461875506
38-39	20.877982568938418	27.487260084774018	28.66242796589989	22.972329380387674
40-41	21.03276658570272	27.394389674715434	28.474305853217125	23.09853788636472
42-43	21.112539886650474	27.715864171072056	28.290946325665573	22.880649616611898
44-45	20.836309949040338	27.617040529599468	28.219507548697432	23.327141972662762
46-47	20.879173215221222	27.863504310139543	28.423108063056628	22.834214411582607
48-49	20.65175977520598	27.62537505357908	28.721960280040005	23.00090489117493
50-51	20.67676334714483	27.814687812544648	28.379054150592943	23.12949468971758
52-53	20.648187836357575	27.662285088345957	28.73981997428204	22.94970710101443
54-55	20.495785112158877	27.622993761013475	28.442158403581463	23.439062723246177
56-57	20.627946849549936	27.76468066866695	28.45406486640949	23.153307615373624
58-59	20.64104395866076	27.61346859075106	28.78982711815974	22.955660332428444
60-61	20.570795827975424	27.501547840167646	28.406439015097394	23.52121731675954
62-63	20.366004667333428	27.827784921655475	28.78149259418012	23.024717816830975
64-65	20.699385626518072	27.909939515168837	28.139734247749676	23.250940610563415
66-67	20.570795827975424	27.607515359337047	28.50645330285279	23.31523550983474
68-69	20.420774396342335	27.553936276610948	28.557651093013288	23.467638234033434
70-71	21.288755536505217	27.478925560794398	27.993284754964996	23.239034147735392
72-73	20.70652950421489	27.275325046435206	28.3564318712197	23.661713578130207
74-75	21.04824498737915	27.55512692289375	28.102824212982807	23.293803876744295
76	20.802971853121875	27.644425394103923	27.801590703433828	23.751012049340382
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	1.5
12	3.0
13	3.0
14	4.0
15	10.5
16	22.5
17	29.0
18	43.0
19	67.5
20	123.5
21	169.0
22	191.0
23	246.0
24	313.0
25	347.0
26	404.5
27	510.0
28	627.5
29	697.0
30	779.5
31	922.0
32	1084.5
33	1187.0
34	1291.0
35	1510.0
36	1724.5
37	1824.0
38	1864.5
39	2033.0
40	2236.0
41	2228.5
42	2146.0
43	2182.5
44	2180.0
45	2098.5
46	2056.0
47	2036.0
48	1876.0
49	1692.0
50	1648.0
51	1490.0
52	1225.5
53	1087.5
54	1056.0
55	1003.5
56	894.5
57	789.0
58	740.0
59	663.5
60	552.0
61	471.5
62	426.0
63	402.0
64	342.0
65	310.5
66	301.0
67	287.0
68	274.0
69	259.5
70	263.5
71	269.0
72	248.5
73	215.5
74	190.5
75	178.0
76	170.0
77	141.0
78	110.5
79	101.0
80	87.0
81	66.5
82	51.0
83	42.0
84	31.0
85	16.0
86	9.5
87	7.0
88	6.5
89	5.0
90	3.5
91	2.0
92	1.0
93	0.5
94	0.5
95	1.0
96	1.0
97	0.5
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	41994.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.31833119017003
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.78722369181185	64.05438872219841
2	13.135090759828513	20.57436776682383
3	3.037489738210344	7.136733819117016
4	1.061145063699109	3.324284421584036
5	0.4499984797348658	1.7621564985474114
6	0.2128371187935176	1.0001428775539363
7	0.13986439235002585	0.7667762061246846
8	0.06081060536957645	0.3810068104967376
9	0.04256742375870352	0.30004286326618085
>10	0.07297272644349174	0.7001000142877554
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.00476258513120922	0.0
24	0.0	0.0	0.0	0.01190646282802305	0.0
25	0.0	0.0	0.0	0.01190646282802305	0.0
26	0.0	0.0	0.0	0.01190646282802305	0.0
27	0.0	0.0	0.0	0.01905034052483688	0.0
28	0.0	0.0	0.0	0.028575510787255322	0.0
29	0.0	0.0	0.0	0.040481973615278374	0.0
30	0.0	0.0	0.0	0.050007143877696815	0.0
31	0.0	0.0	0.0	0.057151021574510644	0.0
32	0.0	0.0	0.0	0.06429489927132448	0.0
33	0.0	0.0	0.0	0.08572653236176597	0.0
34	0.0	0.0	0.0	0.11192075058341668	0.0
35	0.0	0.0	0.0	0.15240272419869505	0.0
36	0.0	0.0	0.0	0.20717245320760108	0.0
37	0.0	0.0	0.0	0.2857551078725532	0.0
38	0.0	0.0	0.0	0.3810068104967376	0.0
39	0.0	0.0	0.0	0.4834023908177359	0.0
40	0.0	0.0	0.0	0.6215173596228033	0.0
41	0.0	0.0	0.0	0.7120064771157785	0.0
42	0.0	0.0	0.0	0.8572653236176596	0.0
43	0.0	0.0	0.0	1.019193218078773	0.0
44	0.0	0.0	0.0	1.2549411820736296	0.0
45	0.0	0.0	0.0	1.447825879887603	0.0
46	0.0	0.0	0.0	1.7050054769729008	0.0
47	0.0	0.0	0.0	1.9193218078773158	0.0
48	0.0	0.0	0.0	2.1122065056912893	0.0
49	0.0	0.0	0.0	2.3384292994237272	0.0
50	0.0	0.0	0.0	2.5789398485497927	0.0
51	0.0	0.0	0.0	2.77896842406058	0.0
52	0.0	0.0	0.0	2.967090536743344	0.0
53	0.0	0.0	0.0	3.212363671000619	0.0
54	0.0	0.0	0.0	3.469543268085917	0.0
55	0.0	0.0	0.0	3.6862408915559364	0.0
56	0.0	0.0	0.0	3.8767442968043055	0.0
57	0.0	0.0	0.0	4.102967090536743	0.0
58	0.0	0.0	0.0	4.36490927275325	0.0
59	0.0	0.0	0.0	4.524455874648759	0.0
60	0.0	0.0	0.0	4.712577987331524	0.0
61	0.0	0.0	0.0	4.910225270276706	0.0
62	0.0	0.0	0.0	5.115016430918702	0.0
63	0.0	0.0	0.0	5.310282421298281	0.0
64	0.0	0.0	0.0	5.512692289374672	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGGG	15	0.002209951	70.0	11
TTGTCTA	15	0.002209951	70.0	20
AGAACTG	15	0.002209951	70.0	37
GTCCCAA	25	2.3644877E-4	55.999996	51
TTAATGT	20	0.0069056684	52.500004	5
CCAGGAT	20	0.0069056684	52.500004	3
GAGAACT	20	0.0069056684	52.500004	36
TTTTCAC	20	0.0069056684	52.500004	47
TATATCC	40	0.002393048	35.000004	5
CCACCAA	45	0.0042617605	31.111109	47
AATATTA	50	0.0071326876	27.999998	53
TATTATA	85	0.0037311092	20.588236	2
>>END_MODULE