FastQCFastQC Report
Wed 25 May 2016
SRR1781139_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781139_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41154
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT2360.573455800165233No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG420.10205569324974485No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTAAG150.002209649770.014
CGTATGC601.8189894E-1258.33333241
CTCGTAT601.8189894E-1258.33333239
TATGCCG601.8189894E-1258.33333243
GTATGCC601.8189894E-1258.33333242
TCGTATG601.8189894E-1258.33333240
ATCTCGT553.45608E-1157.2727337
CATCTCG553.45608E-1157.2727336
ACACCTC700.055.030
CCGTCTT608.0035534E-1152.547
TCTCGTA608.0035534E-1152.538
GGCTGAT200.006904735752.565
ATGCCGT707.2759576E-1250.044
TGCCGTC651.7644197E-1048.46153645
ACTACAC800.048.12527
TACACCT800.048.12529
ACCTCAT751.4551915E-1146.66666832
CTTGAAA751.4551915E-1146.66666857
CGTCTTC604.7439244E-946.66666448
GCCGTCT604.7439244E-946.66666446
Produced by FastQC (version 0.11.5)