##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781139_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	41154
Sequences flagged as poor quality	0
Sequence length	76
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.76143266754143	34.0	34.0	34.0	31.0	34.0
2	32.96863002381299	34.0	34.0	34.0	31.0	34.0
3	33.066627788307336	34.0	34.0	34.0	31.0	34.0
4	33.15570782912961	34.0	34.0	34.0	32.0	34.0
5	33.16416387228459	34.0	34.0	34.0	32.0	34.0
6	36.17993390678913	38.0	37.0	38.0	34.0	38.0
7	36.100063177333915	38.0	36.0	38.0	32.0	38.0
8	35.69830393157409	38.0	36.0	38.0	31.0	38.0
9	35.819434319871704	38.0	36.0	38.0	31.0	38.0
10-11	35.72966175827381	38.0	36.0	38.0	31.0	38.0
12-13	35.668853574379156	38.0	36.0	38.0	31.0	38.0
14-15	35.744775720464595	38.0	36.0	38.0	31.0	38.0
16-17	35.65314428731108	38.0	36.0	38.0	31.0	38.0
18-19	35.65203868396754	38.0	36.0	38.0	31.0	38.0
20-21	35.5537371822909	38.0	36.0	38.0	31.0	38.0
22-23	35.62051076444574	38.0	36.0	38.0	31.0	38.0
24-25	35.59855664090975	38.0	36.0	38.0	31.0	38.0
26-27	35.38704621664966	38.0	36.0	38.0	31.0	38.0
28-29	35.33352772513	38.0	36.0	38.0	31.0	38.0
30-31	35.36804441852554	38.0	36.0	38.0	31.0	38.0
32-33	35.28047091412742	37.5	36.0	38.0	31.0	38.0
34-35	35.29669047966176	37.5	36.0	38.0	30.5	38.0
36-37	35.24843271613938	37.0	35.5	38.0	29.0	38.0
38-39	35.249161685376876	37.5	36.0	38.0	30.0	38.0
40-41	35.25109345385624	37.0	36.0	38.0	30.0	38.0
42-43	35.21106089323031	37.0	35.5	38.0	28.5	38.0
44-45	35.19900860183701	37.0	35.0	38.0	27.0	38.0
46-47	35.231204743159836	37.0	35.5	38.0	28.5	38.0
48-49	35.24154395684502	37.0	35.5	38.0	29.5	38.0
50-51	35.08267726101958	37.0	35.0	38.0	27.0	38.0
52-53	35.11186032949409	37.0	35.0	38.0	27.0	38.0
54-55	35.14787140982651	37.0	35.0	38.0	27.0	38.0
56-57	35.055523156922774	37.0	35.0	38.0	27.0	38.0
58-59	35.08951742236478	37.0	35.0	38.0	27.0	38.0
60-61	35.0711838460417	37.0	35.0	38.0	27.0	38.0
62-63	35.04509889682655	37.0	35.0	38.0	27.0	38.0
64-65	34.994775720464595	37.0	35.0	38.0	27.0	38.0
66-67	34.9774870000486	37.0	35.0	38.0	27.0	38.0
68-69	34.88614715459008	37.0	35.0	38.0	27.0	38.0
70-71	34.856150070467024	37.0	35.0	38.0	27.0	38.0
72-73	34.83629780823249	37.0	35.0	38.0	27.0	38.0
74-75	34.814295086747336	37.0	35.0	38.0	27.0	38.0
76	33.82470719735627	37.0	34.0	38.0	24.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	1.0
21	3.0
22	8.0
23	27.0
24	57.0
25	94.0
26	223.0
27	305.0
28	577.0
29	824.0
30	1241.0
31	1719.0
32	2473.0
33	3237.0
34	4486.0
35	5824.0
36	8246.0
37	11808.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.337026777469994	19.878991106575302	9.32594644506002	25.45803567089469
2	21.550760557904454	22.447392720027214	33.571463284249404	22.430383437818925
3	20.017495261699956	24.51037566214706	30.361568741799093	25.11056033435389
4	14.139573310006318	19.098994022452253	37.335374447198326	29.426058220343105
5	13.468921611507994	35.7996792535355	36.26864946299266	14.462749671963843
6	30.04568207221655	38.963405744277594	17.927783447538516	13.063128735967341
7	27.406813432473147	31.1464256208388	23.00383923798416	18.442921708703892
8	24.031685862856587	38.146960198279636	19.567964231909414	18.25338970695437
9	24.228507556981096	16.409097536083976	21.95655343344511	37.40584147348982
10-11	24.415609661272295	26.485882295767123	28.305875492054234	20.79263255090635
12-13	24.241871993001894	23.97215337512757	28.355688389949947	23.43028624192059
14-15	22.095057588569762	24.978130922875057	24.7594401516256	28.16737133692958
16-17	22.800942800213832	29.04699421684405	25.778782135393886	22.373280847548234
18-19	22.61748554211012	28.65335082859503	26.828497837391264	21.90066579190358
20-21	22.3076736161734	28.302230645866743	26.809058657724645	22.581037080235212
22-23	22.55309325946445	28.185595567867033	27.30111289303591	21.9601982796326
24-25	22.798512902755505	27.670457306701657	27.35943043203577	22.17159935850707
26-27	22.25786071827769	28.02279243815911	27.891577975409437	21.82776886815376
28-29	21.951693638528454	27.839335180055404	28.545220391699473	21.663750789716673
30-31	22.481411284443798	28.22325897847111	27.445691791806386	21.84963794527871
32-33	21.615152840550127	28.338679107741655	27.68746658890995	22.35870146279827
34-35	22.413374155610633	27.330271662535843	28.01914759197162	22.237206589881907
36-37	22.232346794965252	27.88793312922195	27.686251640180785	22.193468435632017
38-39	21.917675074111873	27.846624872430382	28.320454876804195	21.915245176653546
40-41	21.724498226174855	27.353355688389954	28.915779754094377	22.006366331340818
42-43	22.299168975069254	27.414103124848133	28.462603878116344	21.824124021966274
44-45	21.757301841862272	27.693541332555764	28.4273703649706	22.12178646061136
46-47	22.481411284443798	27.50400933080624	27.99241871993002	22.022160664819946
48-49	21.550760557904454	27.49428974097293	28.49176264761627	22.463187053506342
50-51	21.768236380424746	27.68746658890995	28.625407007824272	21.918890022841037
52-53	21.33328473538417	27.655877921951692	29.490450502988775	21.520386839675364
54-55	21.300481119696748	27.38737425280653	29.19400301307285	22.118141614423873
56-57	21.728143072362347	27.366720124410747	28.10783884920056	22.79729795402634
58-59	21.240948631967733	27.366720124410747	29.457646887301358	21.934684356320165
60-61	21.63216212275842	28.336249210283327	28.121203285221362	21.91038538173689
62-63	20.957865578072603	28.622977110365944	28.563444622636926	21.855712688924527
64-65	20.903192885260243	28.721387957428195	28.075035233513145	22.300383923798417
66-67	20.87524906448948	28.81979880449045	27.920736744909362	22.384215386110707
68-69	20.876464013218644	28.925499343927687	28.071390387325657	22.12664625552802
70-71	21.35150896632162	28.586528648491033	27.616999562618457	22.444962822568886
72-73	21.308985760800894	28.071390387325657	27.82475579530544	22.794868056568014
74-75	21.100014579384748	27.699616076201583	27.957185206784274	23.24318413762939
76	21.541040968071147	27.892792924138604	27.426252612139766	23.139913495650482
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	0.5
10	0.0
11	1.0
12	2.0
13	3.5
14	3.0
15	15.0
16	42.0
17	55.0
18	64.5
19	88.0
20	140.0
21	178.0
22	185.0
23	245.5
24	358.0
25	417.0
26	440.5
27	492.5
28	601.5
29	682.0
30	785.5
31	954.0
32	1132.0
33	1245.0
34	1325.0
35	1480.5
36	1642.0
37	1728.0
38	1814.5
39	1999.0
40	2099.0
41	2137.5
42	2174.0
43	2255.0
44	2290.0
45	2134.5
46	2025.0
47	1993.0
48	1828.0
49	1574.0
50	1453.0
51	1391.5
52	1212.5
53	1055.5
54	1016.0
55	954.0
56	803.5
57	705.5
58	696.0
59	615.5
60	493.0
61	419.5
62	388.0
63	379.0
64	344.0
65	300.0
66	285.5
67	289.0
68	283.0
69	250.5
70	218.5
71	213.0
72	209.0
73	210.0
74	199.0
75	183.0
76	175.5
77	144.0
78	108.5
79	97.0
80	88.5
81	66.0
82	41.0
83	30.0
84	30.5
85	23.0
86	10.5
87	6.0
88	6.5
89	4.0
90	1.5
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	41154.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.10905379792973
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.96920205319645	64.02536812946494
2	13.093793747083527	20.45487680419886
3	3.0611292580494633	7.173057296982067
4	0.9457147301291025	2.954755309325946
5	0.41063929071395244	1.6037323224959907
6	0.20220874163944624	0.9476600087476309
7	0.11199253383107792	0.6123341594984691
8	0.07777259293824856	0.4859794916654517
9	0.052885363198009024	0.37177431112407056
>10	0.07155078550318868	0.7970063663313409
>50	0.0	0.0
>100	0.003110903717529942	0.573455800165233
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT	236	0.573455800165233	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG	42	0.10205569324974485	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0024298974583272584	0.0
25	0.0	0.0	0.0	0.0024298974583272584	0.0
26	0.0	0.0	0.0	0.0024298974583272584	0.0
27	0.0	0.0	0.0	0.009719589833309034	0.0
28	0.0	0.0	0.0	0.014579384749963552	0.0
29	0.0	0.0	0.0	0.026728872041599846	0.0
30	0.0	0.0	0.0	0.036448461874908876	0.0
31	0.0	0.0	0.0	0.04373815424989065	0.0
32	0.0	0.0	0.0	0.05102784662487243	0.0
33	0.0	0.0	0.0	0.07289692374981775	0.0
34	0.0	0.0	0.0	0.09476600087476308	0.0
35	0.0	0.0	0.0	0.12878456529134472	0.0
36	0.0	0.0	0.0	0.17738251445788988	0.0
37	0.0	0.0	0.0	0.25513923312436215	0.0
38	0.0	0.0	0.0	0.3353258492491617	0.0
39	0.0	0.0	0.0	0.42766195266559753	0.0
40	0.0	0.0	0.0	0.5564465179569422	0.0
41	0.0	0.0	0.0	0.6414929289983963	0.0
42	0.0	0.0	0.0	0.7775671866647228	0.0
43	0.0	0.0	0.0	0.920931136706031	0.0
44	0.0	0.0	0.0	1.1420518054138116	0.0
45	0.0	0.0	0.0	1.3170044224133741	0.0
46	0.0	0.0	0.0	1.5624240657044273	0.0
47	0.0	0.0	0.0	1.7519560674539534	0.0
48	0.0	0.0	0.0	1.943917966661807	0.0
49	0.0	0.0	0.0	2.1480293531612964	0.0
50	0.0	0.0	0.0	2.3885892015356953	0.0
51	0.0	0.0	0.0	2.5708315109102395	0.0
52	0.0	0.0	0.0	2.755503717743111	0.0
53	0.0	0.0	0.0	2.9960635661175097	0.0
54	0.0	0.0	0.0	3.24391310686689	0.0
55	0.0	0.0	0.0	3.44802449336638	0.0
56	0.0	0.0	0.0	3.637556495115906	0.0
57	0.0	0.0	0.0	3.863536958740341	0.0
58	0.0	0.0	0.0	4.106526704573067	0.0
59	0.0	0.0	0.0	4.2790494241143024	0.0
60	0.0	0.0	0.0	4.466151528405502	0.0
61	0.0	0.0	0.0	4.655683530155027	0.0
62	0.0	0.0	0.0	4.85736501919619	0.0
63	0.0	0.0	0.0	5.046897020945716	0.0
64	0.0	0.0	0.0	5.241288817611896	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGTAAG	15	0.0022096497	70.0	14
CGTATGC	60	1.8189894E-12	58.333332	41
CTCGTAT	60	1.8189894E-12	58.333332	39
TATGCCG	60	1.8189894E-12	58.333332	43
GTATGCC	60	1.8189894E-12	58.333332	42
TCGTATG	60	1.8189894E-12	58.333332	40
ATCTCGT	55	3.45608E-11	57.27273	37
CATCTCG	55	3.45608E-11	57.27273	36
ACACCTC	70	0.0	55.0	30
CCGTCTT	60	8.0035534E-11	52.5	47
TCTCGTA	60	8.0035534E-11	52.5	38
GGCTGAT	20	0.0069047357	52.5	65
ATGCCGT	70	7.2759576E-12	50.0	44
TGCCGTC	65	1.7644197E-10	48.461536	45
ACTACAC	80	0.0	48.125	27
TACACCT	80	0.0	48.125	29
ACCTCAT	75	1.4551915E-11	46.666668	32
CTTGAAA	75	1.4551915E-11	46.666668	57
CGTCTTC	60	4.7439244E-9	46.666664	48
GCCGTCT	60	4.7439244E-9	46.666664	46
>>END_MODULE