FastQCFastQC Report
Wed 25 May 2016
SRR1781138_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781138_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134233
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATA150.002220612770.0000121
CCGATAA150.002220612770.0000122
TTCCGAT150.002220612770.0000120
GGTATGA150.002220612770.000011
GAACGAC150.002220612770.0000136
CAAGAAT150.002220612770.0000136
ACGCAGC200.006938731352.539
TGCGCGA200.006938731352.512
GTATCCA200.006938731352.513
CGTTTGG200.006938731352.528
ATGTGAC305.8607565E-446.66666816
GACGGAT404.5730398E-543.757
GGACGGA404.5730398E-543.756
ATTCCGA350.001251810540.019
GGTATTG350.001251810540.033
GGGCTAA400.002411852635.06
GTCGGTT400.002411852635.070
TCTAGTA552.979486E-431.8181843
ACGGATC552.979486E-431.8181848
CCTAGTC450.00429504431.1111132
Produced by FastQC (version 0.11.5)