##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781138_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	134233
Sequences flagged as poor quality	0
Sequence length	76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.617240171939837	34.0	33.0	34.0	24.0	34.0
2	31.921986396787677	34.0	34.0	34.0	27.0	34.0
3	31.996982858164536	34.0	34.0	34.0	27.0	34.0
4	32.07911616368553	34.0	34.0	34.0	27.0	34.0
5	32.10076508757161	34.0	34.0	34.0	27.0	34.0
6	35.17673746396191	38.0	36.0	38.0	27.0	38.0
7	35.138512884313094	38.0	36.0	38.0	27.0	38.0
8	34.920414503140066	38.0	36.0	38.0	27.0	38.0
9	35.21565486877295	38.0	36.0	38.0	27.0	38.0
10-11	35.104746969821136	38.0	36.0	38.0	27.0	38.0
12-13	35.0678000193693	38.0	36.0	38.0	27.0	38.0
14-15	35.01680287261702	38.0	36.0	38.0	27.0	38.0
16-17	34.973132538198506	38.0	36.0	38.0	27.0	38.0
18-19	35.003341205217794	38.0	36.0	38.0	27.0	38.0
20-21	34.93818956590406	38.0	36.0	38.0	27.0	38.0
22-23	34.995425863982774	38.0	36.0	38.0	27.0	38.0
24-25	35.01544329635782	38.0	36.0	38.0	27.0	38.0
26-27	34.942823299784706	38.0	36.0	38.0	27.0	38.0
28-29	35.022706785961724	38.0	36.0	38.0	27.0	38.0
30-31	35.08325076546006	38.0	36.0	38.0	27.0	38.0
32-33	35.052971325977964	38.0	36.0	38.0	27.0	38.0
34-35	35.07758896843548	38.0	36.0	38.0	27.0	38.0
36-37	34.96545186355069	38.0	36.0	38.0	27.0	38.0
38-39	34.97101308918076	38.0	36.0	38.0	27.0	38.0
40-41	34.94976272600627	38.0	36.0	38.0	27.0	38.0
42-43	35.03075249752297	38.0	36.0	38.0	27.0	38.0
44-45	35.01865785611586	38.0	36.0	38.0	27.0	38.0
46-47	35.01565561374625	38.0	36.0	38.0	27.0	38.0
48-49	34.9745628869205	38.0	36.0	38.0	27.0	38.0
50-51	34.85833215379229	38.0	35.5	38.0	27.0	38.0
52-53	34.93660277279059	38.0	36.0	38.0	27.0	38.0
54-55	34.90861412618358	38.0	36.0	38.0	27.0	38.0
56-57	34.84067256188865	38.0	35.5	38.0	27.0	38.0
58-59	34.849802209590784	38.0	36.0	38.0	27.0	38.0
60-61	34.86058569800272	38.0	35.5	38.0	27.0	38.0
62-63	34.817686410942166	38.0	35.5	38.0	27.0	38.0
64-65	34.84666214716202	38.0	35.5	38.0	27.0	38.0
66-67	34.74697354599837	38.0	35.0	38.0	27.0	38.0
68-69	34.48877697734537	38.0	34.5	38.0	25.0	38.0
70-71	34.69303747960636	38.0	35.0	38.0	26.5	38.0
72-73	34.711967250974055	38.0	35.0	38.0	27.0	38.0
74-75	34.71509241393696	38.0	35.0	38.0	27.0	38.0
76	33.50326670788852	37.0	33.0	38.0	23.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	18.0
14	58.0
15	142.0
16	291.0
17	442.0
18	581.0
19	599.0
20	673.0
21	663.0
22	808.0
23	928.0
24	1077.0
25	1279.0
26	1467.0
27	1793.0
28	2244.0
29	2714.0
30	3301.0
31	4159.0
32	5354.0
33	6995.0
34	9414.0
35	13585.0
36	22596.0
37	53051.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.705109771814676	17.957581220713237	8.820483785656284	25.516825221815797
2	23.188783682104997	20.23272965664181	32.04279126593312	24.53569539532008
3	19.38941988929697	21.827717476328473	30.493246817101603	28.28961581727295
4	13.271699209583337	16.51009811298265	37.66435973270358	32.553842944730434
5	13.300753167998927	33.70482668196345	36.949930344997135	16.04448980504049
6	29.775837536224326	38.036101405764605	18.561754561098983	13.626306496912086
7	28.868460065706646	30.126719957089538	22.130921606460408	18.873898370743408
8	25.887076948291405	35.30651926128448	19.338761705392862	19.46764208503125
9	24.90445717521027	16.232968048095476	21.237698628504166	37.62487614819009
10-11	24.83778206551295	24.702941899532902	28.383109965507735	22.07616606944641
12-13	24.94058837990658	23.0293593974656	27.75770488627983	24.27234733634799
14-15	23.075547741613462	24.138997116953355	24.83442968569577	27.951025455737412
16-17	23.05133610960047	27.83778951524588	25.749256889140526	23.361617486013124
18-19	22.309715196710197	27.48206476797807	27.210149516139847	22.998070519171886
20-21	22.54289183732763	27.479084874807235	27.02092629979215	22.957096988072976
22-23	22.610684406964012	26.718467142952925	27.447423509867168	23.223424940215892
24-25	22.61664419330567	27.131182347112855	27.100265955465495	23.151907504115975
26-27	21.92158411120961	27.314818263765243	27.274217219312686	23.489380405712453
28-29	22.333181855430485	27.23249871492107	28.06575134281436	22.368568086834088
30-31	22.726900240626374	27.455990702733306	27.448540969806235	22.368568086834088
32-33	22.08808564212973	27.679855177191897	27.136769646808162	23.09528953387021
34-35	22.611801866903072	26.869324234726182	27.739080553962143	22.779793344408603
36-37	22.681829356417573	27.016083973389556	27.189290263944038	23.112796406248837
38-39	22.151408372009865	27.54129014474831	27.692519723167923	22.614781760073903
40-41	22.450142662385552	26.735229042038846	28.092942867998183	22.72168542757742
42-43	22.211751208719168	27.166196091870106	27.683580043655436	22.93847265575529
44-45	21.860868787853953	27.200464863334652	27.638509159446635	23.30015718936476
46-47	22.043387244567285	26.68308091154932	27.861256173966165	23.412275669917236
48-49	21.847831755231574	26.89800570649542	28.209903674953253	23.04425886331975
50-51	22.069833796458397	26.81382372441948	27.939850856346798	23.176491622775323
52-53	21.66047097211565	26.45102173087095	28.431533229533724	23.45697406747968
54-55	21.47869748869503	27.105108281868095	28.269501538369852	23.146692691067024
56-57	21.434371577778936	26.76875284021068	28.18531955629391	23.611556025716478
58-59	21.432136657900813	26.67674863856131	28.351076113921316	23.540038589616564
60-61	21.252225607711964	26.838780329725182	28.178242310013186	23.73075175254967
62-63	20.87824901477282	26.923334798447474	28.488151199779487	23.710264987000215
64-65	21.136754747342305	26.892790893446474	28.190534369342856	23.779919989868365
66-67	21.287239352469214	26.426065125565252	28.21288356812408	24.073811953841453
68-69	21.149046806671983	26.70468513703784	27.88584029262551	24.260427763664673
70-71	21.666803245103665	26.667436472402468	27.908561978053086	23.757198304440784
72-73	21.489499601439288	26.476350822823	27.737963094023076	24.296186481714628
74-75	21.638494259980778	26.48417304239643	27.837417028599525	24.039915669023266
76	21.70852174949528	26.580647083802045	27.38000342687715	24.330827739825526
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	2.0
5	1.0
6	0.0
7	0.5
8	1.0
9	1.0
10	1.0
11	0.5
12	0.0
13	0.5
14	2.5
15	20.0
16	43.5
17	51.0
18	76.0
19	122.5
20	178.5
21	213.0
22	292.0
23	429.0
24	597.5
25	708.0
26	865.0
27	1092.5
28	1364.0
29	1565.0
30	1748.5
31	2159.0
32	2711.0
33	3036.0
34	3305.5
35	3892.0
36	4510.0
37	4811.0
38	5236.5
39	6102.0
40	6941.5
41	7502.5
42	7664.0
43	7767.5
44	7898.0
45	7850.5
46	7776.0
47	7683.0
48	7197.5
49	6418.0
50	6031.0
51	5638.0
52	4794.0
53	4157.5
54	3972.0
55	3682.5
56	3177.5
57	2701.5
58	2441.0
59	2209.0
60	1852.0
61	1536.0
62	1345.0
63	1232.5
64	1071.0
65	957.0
66	876.0
67	860.0
68	859.5
69	819.5
70	733.0
71	686.0
72	679.0
73	634.5
74	572.0
75	547.0
76	485.5
77	392.0
78	327.0
79	294.0
80	256.0
81	181.5
82	131.0
83	117.0
84	97.5
85	62.0
86	42.5
87	39.0
88	28.0
89	14.0
90	9.0
91	5.0
92	3.0
93	1.5
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	3.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	134233.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.333152056498776
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.91927099411016	44.59782616793188
2	15.256769604998333	18.409780009386665
3	4.715571634953758	8.535159014549329
4	1.9151221801029794	4.6218143079570595
5	1.0458468643115562	3.1549618946160782
6	0.6655389136528085	2.409243628615914
7	0.5148974526775902	2.1745770414130656
8	0.3346216059367553	1.6151020985897657
9	0.29017002728833025	1.5756185140762702
>10	1.3335473594527516	12.612397845537238
>50	0.00864336251497154	0.29351947732673783
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.001489946585414913	0.0
10	0.0	0.0	0.0	0.001489946585414913	0.0
11	0.0	0.0	0.0	0.001489946585414913	0.0
12	0.0	0.0	0.0	0.001489946585414913	0.0
13	0.0	0.0	0.0	0.001489946585414913	0.0
14	0.0	0.0	0.0	0.001489946585414913	0.0
15	0.0	0.0	0.0	0.001489946585414913	0.0
16	0.0	0.0	0.0	0.001489946585414913	0.0
17	0.0	0.0	0.0	0.001489946585414913	0.0
18	0.0	0.0	0.0	0.0022349198781223692	0.0
19	0.0	0.0	0.0	0.002979893170829826	0.0
20	0.0	0.0	0.0	0.003724866463537282	0.0
21	0.0	0.0	0.0	0.0044698397562447385	0.0
22	0.0	0.0	0.0	0.005214813048952195	0.0
23	0.0	0.0	0.0	0.005214813048952195	0.0
24	0.0	0.0	0.0	0.006704759634367108	0.0
25	0.0	0.0	0.0	0.00819470621978202	0.0
26	0.0	0.0	0.0	0.008939679512489477	0.0
27	0.0	0.0	0.0	0.011919572683319304	0.0
28	0.0	0.0	0.0	0.0171343857322715	0.0
29	0.0	0.0	0.0	0.02085925219580878	0.0
30	0.0	0.0	0.0	0.023839145366638607	0.0
31	0.0	0.0	0.0	0.034268771464543	0.0
32	0.0	0.0	0.0	0.04991321061139958	0.0
33	0.0	0.0	0.0	0.06704759634367108	0.0
34	0.0	0.0	0.0	0.07971214231969784	0.0
35	0.0	0.0	0.0	0.11547086036965575	0.0
36	0.0	0.0	0.0	0.1624041778102255	0.0
37	0.0	0.0	0.0	0.2428612934226308	0.0
38	0.0	0.0	0.0	0.3352379817183554	0.0
39	0.0	0.0	0.0	0.4596485216005006	0.0
40	0.0	0.0	0.0	0.598958527336795	0.0
41	0.0	0.0	0.0	0.7583828119761906	0.0
42	0.0	0.0	0.0	0.9312166158843206	0.0
43	0.0	0.0	0.0	1.1770578024777811	0.0
44	0.0	0.0	0.0	1.457167760535785	0.0
45	0.0	0.0	0.0	1.7678216235947941	0.0
46	0.0	0.0	0.0	2.102314632020442	0.0
47	0.0	0.0	0.0	2.5060901566678835	0.0
48	0.0	0.0	0.0	2.8897514024122235	0.0
49	0.0	0.0	0.0	3.2972517935232024	0.0
50	0.0	0.0	0.0	3.677188172804005	0.0
51	0.0	0.0	0.0	3.9960367420827962	0.0
52	0.0	0.0	0.0	4.369268361729232	0.0
53	0.0	0.0	0.0	4.762614260278769	0.0
54	0.0	0.0	0.0	5.158940051999136	0.0
55	0.0	0.0	0.0	5.575380122622604	0.0
56	0.0	0.0	0.0	6.030558804466859	0.0
57	0.0	0.0	0.0	6.449978768261158	0.0
58	0.0	0.0	0.0	6.911862209739781	0.0
59	0.0	0.0	0.0	7.305953081582025	0.0
60	0.0	0.0	0.0	7.764111656597111	0.0
61	0.0	0.0	0.0	8.19172632661119	0.0
62	0.0	0.0	0.0	8.574642599062823	0.0
63	0.0	0.0	0.0	8.958303844807164	0.0
64	0.0	0.0	0.0	9.397838087504564	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGATA	15	0.0022206127	70.00001	21
CCGATAA	15	0.0022206127	70.00001	22
TTCCGAT	15	0.0022206127	70.00001	20
GGTATGA	15	0.0022206127	70.00001	1
GAACGAC	15	0.0022206127	70.00001	36
CAAGAAT	15	0.0022206127	70.00001	36
ACGCAGC	20	0.0069387313	52.5	39
TGCGCGA	20	0.0069387313	52.5	12
GTATCCA	20	0.0069387313	52.5	13
CGTTTGG	20	0.0069387313	52.5	28
ATGTGAC	30	5.8607565E-4	46.666668	16
GACGGAT	40	4.5730398E-5	43.75	7
GGACGGA	40	4.5730398E-5	43.75	6
ATTCCGA	35	0.0012518105	40.0	19
GGTATTG	35	0.0012518105	40.0	33
GGGCTAA	40	0.0024118526	35.0	6
GTCGGTT	40	0.0024118526	35.0	70
TCTAGTA	55	2.979486E-4	31.818184	3
ACGGATC	55	2.979486E-4	31.818184	8
CCTAGTC	45	0.004295044	31.111113	2
>>END_MODULE