FastQCFastQC Report
Wed 25 May 2016
SRR1781138_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781138_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124573
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGT6200.4977001436908479No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1350.057.03703741
TATGCCG1350.057.03703743
CGTCTTC1300.056.5384648
TCGTATG1300.056.5384640
CCGTCTT1400.055.047
ATGCCGT1400.055.044
CTCGTAT1350.054.44444339
TGCCGTC1450.053.10344745
ACGTTTA200.006937565752.526
TAACGCT200.006937565752.54
CTGCGGT200.006937565752.530
CGGTCGT200.006937565752.510
CTTACGT200.006937565752.523
CTTACGC200.006937565752.53
TCTCGTA1000.052.49999638
GCCGTCT1500.051.33333246
TCGCAGA1450.050.68965531
ATCTCGT1000.048.99999637
CGCAGAT1100.047.72727232
CCTCGCA1650.046.66666829
Produced by FastQC (version 0.11.5)