##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781138_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	124573
Sequences flagged as poor quality	0
Sequence length	76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.11260867122089	34.0	34.0	34.0	31.0	34.0
2	33.169442816661714	34.0	34.0	34.0	32.0	34.0
3	33.240638019474524	34.0	34.0	34.0	32.0	34.0
4	33.32003724723656	34.0	34.0	34.0	33.0	34.0
5	33.31523684907644	34.0	34.0	34.0	33.0	34.0
6	36.57941929631622	38.0	37.0	38.0	34.0	38.0
7	36.47062365038973	38.0	37.0	38.0	34.0	38.0
8	36.199168359114736	38.0	37.0	38.0	34.0	38.0
9	36.20758912444912	38.0	37.0	38.0	34.0	38.0
10-11	36.12343364934617	38.0	37.0	38.0	32.5	38.0
12-13	36.07031620013968	38.0	37.0	38.0	32.0	38.0
14-15	36.09867708090838	38.0	37.0	38.0	32.0	38.0
16-17	36.019362141073906	38.0	36.0	38.0	32.0	38.0
18-19	36.02199513538247	38.0	36.0	38.0	32.0	38.0
20-21	35.961343950936396	38.0	36.0	38.0	32.0	38.0
22-23	36.01855939890667	38.0	36.0	38.0	32.0	38.0
24-25	36.037291387379284	38.0	36.0	38.0	32.0	38.0
26-27	35.806017355285654	38.0	36.0	38.0	31.5	38.0
28-29	35.749187223555666	38.0	36.0	38.0	31.0	38.0
30-31	35.79514822634118	38.0	36.0	38.0	31.0	38.0
32-33	35.74935981312162	38.0	36.0	38.0	31.0	38.0
34-35	35.758466923008996	38.0	36.0	38.0	31.0	38.0
36-37	35.70591942074125	38.0	36.0	38.0	31.0	38.0
38-39	35.69155434965843	38.0	36.0	38.0	31.0	38.0
40-41	35.7092708692895	38.0	36.0	38.0	31.0	38.0
42-43	35.6935893010524	38.0	36.0	38.0	31.0	38.0
44-45	35.66914580205984	38.0	36.0	38.0	31.0	38.0
46-47	35.69298323071612	38.0	36.0	38.0	31.0	38.0
48-49	35.68101835871336	38.0	36.0	38.0	31.0	38.0
50-51	35.56696876530227	38.0	36.0	38.0	31.0	38.0
52-53	35.594908206433175	38.0	36.0	38.0	31.0	38.0
54-55	35.601735528565584	38.0	36.0	38.0	31.0	38.0
56-57	35.546177743170674	38.0	36.0	38.0	31.0	38.0
58-59	35.57200195869089	38.0	36.0	38.0	31.0	38.0
60-61	35.542208183153654	38.0	36.0	38.0	31.0	38.0
62-63	35.528702046189785	38.0	36.0	38.0	31.0	38.0
64-65	35.46109911457539	38.0	36.0	38.0	31.0	38.0
66-67	35.48458735038893	38.0	36.0	38.0	31.0	38.0
68-69	35.416085347547224	38.0	36.0	38.0	31.0	38.0
70-71	35.37844476732518	38.0	36.0	38.0	31.0	38.0
72-73	35.336328899520765	38.0	36.0	38.0	30.5	38.0
74-75	35.36481019161455	38.0	36.0	38.0	31.0	38.0
76	34.46233132380211	37.0	34.0	38.0	26.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	7.0
22	17.0
23	38.0
24	92.0
25	182.0
26	397.0
27	656.0
28	1180.0
29	1866.0
30	3001.0
31	4289.0
32	6163.0
33	8583.0
34	11619.0
35	15712.0
36	23495.0
37	47275.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.69230089987397	18.68382394258788	9.197819752273768	25.426055405264382
2	23.600619716953112	21.154664333362767	30.89754601719474	24.347169932489386
3	20.119126937618905	22.915077906127333	29.816252317918007	27.149542838335755
4	13.864159970459088	17.367326788308862	37.41260144654139	31.355911794690662
5	13.20109494031612	35.07822722419786	36.44208616634423	15.27859166914179
6	30.114872404132516	38.48907869281465	18.070528926813996	13.32551997623883
7	28.695624252446354	30.580462861133633	21.773578544307355	18.950334342112658
8	25.460573318455843	36.033490403217385	19.337256066723928	19.168680211602833
9	24.60404742600724	16.0981914218972	20.691482102863382	38.60627904923218
10-11	24.617292671766755	25.425654034180763	28.21518306535124	21.741870228701245
12-13	24.693151806571247	23.430037006413908	27.572186589389354	24.304624597625487
14-15	22.64816613551893	24.47440456599745	24.48042513225177	28.39700416623185
16-17	23.200452746582325	28.284620262817782	25.37146893789184	23.143458052708052
18-19	22.656594928274988	27.800165364886453	26.663081084986313	22.88015862185225
20-21	22.33389257704318	27.47545615823654	26.596854856188738	23.593796408531546
22-23	22.55785764170406	27.243865042986844	27.19891148162122	22.999365833687875
24-25	22.84242973999181	27.317717322373227	26.867780337633356	22.972072600001606
26-27	22.085443876281378	27.85515320334262	27.21416358279884	22.845239337577166
28-29	22.15889478458414	27.010668443402665	27.843914813001213	22.986521959011984
30-31	22.512904080338437	27.09736459746494	27.595064741155788	22.794666581040836
32-33	22.293354097597394	27.36949419216042	27.161583970844404	23.17556773939778
34-35	22.613246851243847	26.91514212550071	27.379528469250964	23.092082554004477
36-37	23.10211683109502	27.456190346222698	26.72047714994421	22.721215672738072
38-39	22.05213007634078	27.55693448821173	27.749592608350127	22.641342827097365
40-41	22.184582533935927	26.87179404846957	28.19872685092275	22.744896566671752
42-43	22.82195981472711	26.80075136666854	27.72671445658369	22.650574362020663
44-45	21.861077440536874	27.308084416366306	27.799362622719208	23.031475520377608
46-47	22.272884172332688	26.773056761898644	27.674134844629254	23.27992422113941
48-49	21.97386271503456	26.75700191855378	27.975163157345488	23.29397220906617
50-51	21.89960906456455	26.85172549428849	28.102397790853555	23.146267650293403
52-53	21.61222736869145	26.47443667568414	28.878649466577833	23.03468648904658
54-55	21.425188443723762	26.65866600306647	28.44356321193196	23.472582341277807
56-57	21.446059740072087	26.631372769380203	28.138922559463126	23.783644931084584
58-59	21.185569906801636	26.485675066025543	28.80840952694404	23.520345500228782
60-61	21.466931036420412	26.811588385926328	28.190699429250323	23.530781148402944
62-63	20.50925963089915	27.307281674199064	28.473666043203583	23.7097926516982
64-65	20.88052788324918	27.54970980870654	27.66329782537147	23.90646448267281
66-67	20.684658794441813	27.21215672738073	27.554927632793625	24.548256845383833
68-69	20.53534875133456	27.67694444221461	27.460204057058913	24.327502749391922
70-71	21.19440007064131	27.324942001878416	27.186067606945326	24.294590320534947
72-73	20.986088478241673	26.515777897297166	27.09013991795975	25.407993706501408
74-75	20.770150835253226	26.92919011342747	26.994212228974174	25.30644682234513
76	21.360969070344296	26.492899745530735	26.75700191855378	25.389129265571192
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	2.5
14	3.5
15	9.5
16	27.0
17	38.0
18	59.5
19	109.0
20	189.0
21	241.0
22	292.5
23	402.0
24	594.5
25	729.0
26	805.5
27	984.5
28	1261.0
29	1435.0
30	1635.5
31	1989.0
32	2502.5
33	2863.0
34	3090.5
35	3605.0
36	4251.5
37	4611.0
38	4895.0
39	5657.5
40	6421.0
41	6877.5
42	7049.0
43	7139.0
44	7368.0
45	7406.5
46	7306.0
47	7251.0
48	6750.5
49	5978.0
50	5651.0
51	5187.0
52	4318.5
53	3826.0
54	3738.0
55	3500.5
56	2966.5
57	2430.5
58	2191.0
59	1993.5
60	1655.5
61	1373.0
62	1231.0
63	1167.0
64	1026.0
65	897.0
66	834.0
67	823.0
68	759.0
69	714.5
70	707.0
71	680.0
72	660.5
73	585.5
74	491.5
75	453.0
76	439.5
77	385.5
78	310.0
79	275.0
80	246.0
81	187.0
82	141.0
83	125.0
84	88.5
85	45.5
86	34.0
87	29.0
88	24.0
89	14.0
90	5.0
91	0.5
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	124573.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.90580623409567
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.71879115547239	40.950286177582626
2	17.216330491439663	19.938509949989164
3	5.387121369654121	9.358368185722428
4	2.1626117695986693	5.0091111235982115
5	1.0466486449019201	3.030351681343469
6	0.761072988147224	2.6442326988994407
7	0.5697650239134955	2.309489215158983
8	0.4034102724059056	1.8687837653424098
9	0.30914257988493793	1.6111035296573095
>10	1.4181742566022042	12.571745081197369
>50	0.005545158383586331	0.21031844781774545
>100	0.0	0.0
>500	0.0013862895958965827	0.4977001436908479
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGT	620	0.4977001436908479	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0016054843344866063	0.0
10	0.0	0.0	0.0	0.0016054843344866063	0.0
11	0.0	0.0	0.0	0.0016054843344866063	0.0
12	0.0	0.0	0.0	0.0016054843344866063	0.0
13	0.0	0.0	0.0	0.0016054843344866063	0.0
14	0.0	0.0	0.0	0.0016054843344866063	0.0
15	0.0	0.0	0.0	0.0016054843344866063	0.0
16	0.0	0.0	0.0	0.0016054843344866063	0.0
17	0.0	0.0	0.0	0.0016054843344866063	0.0
18	0.0	0.0	0.0	0.002408226501729909	0.0
19	0.0	0.0	0.0	0.002408226501729909	0.0
20	0.0	0.0	0.0	0.0032109686689732126	0.0
21	0.0	0.0	0.0	0.0032109686689732126	0.0
22	0.0	0.0	0.0	0.0032109686689732126	0.0
23	0.0	0.0	0.0	0.0032109686689732126	0.0
24	0.0	0.0	0.0	0.0040137108362165155	0.0
25	0.0	0.0	0.0	0.004816453003459818	0.0
26	0.0	0.0	0.0	0.005619195170703122	0.0
27	0.0	0.0	0.0	0.008027421672433031	0.0
28	0.0	0.0	0.0	0.014449359010379456	0.0
29	0.0	0.0	0.0	0.016857585512109367	0.0
30	0.0	0.0	0.0	0.02087129634832588	0.0
31	0.0	0.0	0.0	0.032109686689732124	0.0
32	0.0	0.0	0.0	0.04977001436908479	0.0
33	0.0	0.0	0.0	0.06823308421568076	0.0
34	0.0	0.0	0.0	0.08268244322606022	0.0
35	0.0	0.0	0.0	0.12121406725373877	0.0
36	0.0	0.0	0.0	0.17419505029179677	0.0
37	0.0	0.0	0.0	0.256877493517857	0.0
38	0.0	0.0	0.0	0.3516010692525668	0.0
39	0.0	0.0	0.0	0.48003981601149526	0.0
40	0.0	0.0	0.0	0.627744374784263	0.0
41	0.0	0.0	0.0	0.7923065190691402	0.0
42	0.0	0.0	0.0	0.9721207645316401	0.0
43	0.0	0.0	0.0	1.2217735785443073	0.0
44	0.0	0.0	0.0	1.5115635009191397	0.0
45	0.0	0.0	0.0	1.8414905316561374	0.0
46	0.0	0.0	0.0	2.1971053117449206	0.0
47	0.0	0.0	0.0	2.616136723045925	0.0
48	0.0	0.0	0.0	3.00626941632617	0.0
49	0.0	0.0	0.0	3.424498085459931	0.0
50	0.0	0.0	0.0	3.80820884140223	0.0
51	0.0	0.0	0.0	4.129305708299551	0.0
52	0.0	0.0	0.0	4.507397269071147	0.0
53	0.0	0.0	0.0	4.900740931020366	0.0
54	0.0	0.0	0.0	5.30291475680926	0.0
55	0.0	0.0	0.0	5.719537941608535	0.0
56	0.0	0.0	0.0	6.184325656442407	0.0
57	0.0	0.0	0.0	6.63948046526936	0.0
58	0.0	0.0	0.0	7.109887375273936	0.0
59	0.0	0.0	0.0	7.518483138400777	0.0
60	0.0	0.0	0.0	7.983270853234649	0.0
61	0.0	0.0	0.0	8.427187271720197	0.0
62	0.0	0.0	0.0	8.82695287100736	0.0
63	0.0	0.0	0.0	9.225915728127283	0.0
64	0.0	0.0	0.0	9.685084247790453	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATGC	135	0.0	57.037037	41
TATGCCG	135	0.0	57.037037	43
CGTCTTC	130	0.0	56.53846	48
TCGTATG	130	0.0	56.53846	40
CCGTCTT	140	0.0	55.0	47
ATGCCGT	140	0.0	55.0	44
CTCGTAT	135	0.0	54.444443	39
TGCCGTC	145	0.0	53.103447	45
ACGTTTA	20	0.0069375657	52.5	26
TAACGCT	20	0.0069375657	52.5	4
CTGCGGT	20	0.0069375657	52.5	30
CGGTCGT	20	0.0069375657	52.5	10
CTTACGT	20	0.0069375657	52.5	23
CTTACGC	20	0.0069375657	52.5	3
TCTCGTA	100	0.0	52.499996	38
GCCGTCT	150	0.0	51.333332	46
TCGCAGA	145	0.0	50.689655	31
ATCTCGT	100	0.0	48.999996	37
CGCAGAT	110	0.0	47.727272	32
CCTCGCA	165	0.0	46.666668	29
>>END_MODULE