FastQCFastQC Report
Wed 25 May 2016
SRR1781137_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781137_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93420
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAATCG150.002218492370.0000160
GCTGTCG150.002218492370.0000166
GACTATC150.002218492370.0000150
TGCCAAT200.00693215852.4999962
CCGTGCG200.00693215852.4999969
GTATGGC200.00693215852.4999969
TACTGAC200.00693215852.49999646
GTACTGG200.00693215852.4999961
GAGCAAG200.00693215852.49999626
AAGGCGT200.00693215852.49999623
GGCTCTC200.00693215852.49999665
TCAGTTA200.00693215852.49999618
AGCTTGA350.001249855440.00000457
GGATATA400.0024081134.99999635
TAATCCA400.0024081134.9999964
TTAATCC450.0042884231.1111093
GAATGCT450.0042884231.11110946
AGGCGTG450.0042884231.11110924
CTAGTAA450.0042884231.11110930
GCCCTAC450.0042884231.11110920
Produced by FastQC (version 0.11.5)