FastQCFastQC Report
Wed 25 May 2016
SRR1781137_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781137_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88273
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT5120.580018805297203No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCTGC150.002218085870.016
ATACTGG207.88819E-570.06
GAGCGAA150.002218085870.034
CATGTAC252.3789187E-456.0000043
ATCTCGT452.665547E-854.44444337
GGTAAAC200.00693089452.535
GAATATA200.00693089452.548
ATCGTAC200.00693089452.525
TCGTACT200.00693089452.526
CATCGTA200.00693089452.524
CCGAAAT200.00693089452.547
ACATCGT200.00693089452.523
GTAAGAT200.00693089452.549
CTCTAAT200.00693089452.51
ACTGGTG352.0716972E-550.08
CCGTCTT604.8512447E-946.66666447
ACTAAAT305.849785E-446.66666424
TCTCGTA551.2942473E-744.54545238
CGTATGC659.84619E-943.07692741
CTCGTAT659.84619E-943.07692739
Produced by FastQC (version 0.11.5)