FastQCFastQC Report
Wed 25 May 2016
SRR1781136_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781136_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79367
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGTTG150.002217258370.031
TAATACA252.3774494E-456.0000044
TGGAGGC200.006928328452.513
GCTGTCC200.006928328452.58
TGTCCCC200.006928328452.510
TATCTAT350.001248718539.99999635
CCAGTCA350.001248718539.99999614
GGCTTAA450.00428456431.11110923
TGACAAC450.00428456431.11110939
TTCTCAG450.00428456431.11110956
CATACAC604.94309E-429.1666663
TATACCA604.94309E-429.16666610
ACTGATT500.007170616628.00000242
CCCTAGA500.007170616628.00000269
GTAATAC500.007170616628.0000023
GCCTACA500.007170616628.0000021
AATACCC500.007170616628.0000025
AGATGTG500.007170616628.0000025
ATACCCA657.893867E-426.9230796
CTATACT808.571271E-526.254
Produced by FastQC (version 0.11.5)