##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781136_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	79367
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.50956946841887	34.0	34.0	34.0	31.0	34.0
2	32.52044300527927	34.0	34.0	34.0	31.0	34.0
3	32.45631055728451	34.0	34.0	34.0	31.0	34.0
4	32.56832184661131	34.0	34.0	34.0	31.0	34.0
5	32.62544886413749	34.0	34.0	34.0	31.0	34.0
6	35.745158567162676	38.0	37.0	38.0	31.0	38.0
7	35.708191061776304	38.0	37.0	38.0	31.0	38.0
8	35.55712071767863	38.0	37.0	38.0	31.0	38.0
9	35.894162561266015	38.0	37.0	38.0	32.0	38.0
10-11	35.78923230057833	38.0	37.0	38.0	31.0	38.0
12-13	35.728136379099624	38.0	37.0	38.0	31.0	38.0
14-15	35.68793705192334	38.0	37.0	38.0	31.0	38.0
16-17	35.642641148084216	38.0	37.0	38.0	31.0	38.0
18-19	35.667909836581956	38.0	37.0	38.0	31.0	38.0
20-21	35.65377297869392	38.0	37.0	38.0	31.0	38.0
22-23	35.692787934531985	38.0	37.0	38.0	31.0	38.0
24-25	35.68936081746821	38.0	37.0	38.0	31.0	38.0
26-27	35.621530358965316	38.0	37.0	38.0	31.0	38.0
28-29	35.709243136316104	38.0	37.0	38.0	31.0	38.0
30-31	35.776386911436745	38.0	37.0	38.0	31.0	38.0
32-33	35.724224173775	38.0	37.0	38.0	31.0	38.0
34-35	35.75114972217672	38.0	37.0	38.0	31.0	38.0
36-37	35.67402068869933	38.0	37.0	38.0	31.0	38.0
38-39	35.668061032923006	38.0	37.0	38.0	31.0	38.0
40-41	35.640518099462	38.0	37.0	38.0	31.0	38.0
42-43	35.72162233673945	38.0	37.0	38.0	31.0	38.0
44-45	35.72277520883995	38.0	37.0	38.0	31.0	38.0
46-47	35.69271863620901	38.0	37.0	38.0	31.0	38.0
48-49	35.66748144694898	38.0	37.0	38.0	31.0	38.0
50-51	35.55468897652677	38.0	36.5	38.0	31.0	38.0
52-53	35.61681177315509	38.0	37.0	38.0	31.0	38.0
54-55	35.60329230032633	38.0	37.0	38.0	31.0	38.0
56-57	35.55159575138282	38.0	37.0	38.0	31.0	38.0
58-59	35.5505121776053	38.0	37.0	38.0	31.0	38.0
60-61	35.55715221691635	38.0	37.0	38.0	31.0	38.0
62-63	35.536709211637074	38.0	36.5	38.0	31.0	38.0
64-65	35.55351720488365	38.0	37.0	38.0	31.0	38.0
66-67	35.457967417188506	38.0	36.0	38.0	31.0	38.0
68-69	35.18625499262917	38.0	36.0	38.0	28.0	38.0
70-71	35.39347587788376	38.0	36.0	38.0	30.5	38.0
72-73	35.417642093061346	38.0	36.0	38.0	31.0	38.0
74-75	35.42239847795683	38.0	36.0	38.0	30.5	38.0
76	34.17597994128542	37.0	34.0	38.0	25.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	11.0
14	47.0
15	83.0
16	172.0
17	276.0
18	322.0
19	307.0
20	321.0
21	309.0
22	278.0
23	312.0
24	406.0
25	440.0
26	551.0
27	713.0
28	816.0
29	1004.0
30	1310.0
31	1750.0
32	2370.0
33	3178.0
34	4570.0
35	7181.0
36	13871.0
37	38769.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.81416709715625	17.959605377549863	9.120919273753575	26.10530825154031
2	24.04903801328008	20.061234518124664	30.449683117668553	25.440044350926705
3	20.03855506696738	21.25946552093439	28.521929769299582	30.180049642798647
4	14.175916942809986	16.048231632794487	35.938110297730795	33.83774112666473
5	14.002041150604155	33.46479015207832	35.71005581664924	16.823112880668287
6	30.9335114090239	36.25688258344148	18.051583151687726	14.758022855846889
7	29.64960247961999	28.797863091713182	21.516499300716923	20.0360351279499
8	26.15444706238109	33.52022881046279	19.309032721408144	21.01629140574798
9	24.91841697430922	15.262010659342042	20.48080436453438	39.33876800181436
10-11	25.971751483614096	23.79200423349755	26.958307608955863	23.27793667393249
12-13	25.98750110247332	22.050096387667416	26.059319364471378	25.903083145387885
14-15	23.89154182468784	23.264077009336372	23.46882205450628	29.375559111469503
16-17	24.49947711265387	26.210515705519928	24.43521866770824	24.85478851411796
18-19	23.82728337974221	25.933322413597594	25.661798984464575	24.577595222195622
20-21	23.628838182115995	25.99884082805196	25.69644814595487	24.67587284387718
22-23	23.9898194463694	25.489813146521854	26.23193518716847	24.28843221994028
24-25	23.639547922940267	25.87914372472186	25.72605742941021	24.755250922927665
26-27	22.86718661408394	26.083888769891768	26.36612193984906	24.682802676175235
28-29	23.780034523164538	25.46398377159273	26.93562815779858	23.82035354744415
30-31	23.943200574546093	25.48666322275001	26.28044401325488	24.289692189449017
32-33	23.246437436214045	26.189726208625753	26.088928647926718	24.474907707233484
34-35	24.22732369876649	25.324757140877193	26.36360200083158	24.08431715952474
36-37	24.31174165585193	24.965035846132526	25.993170965262642	24.73005153275291
38-39	23.446142603349	25.961041742789824	26.32517293081507	24.2676427230461
40-41	23.61749845653735	25.190570388196605	26.87199969760732	24.319931457658726
42-43	23.501581261733467	25.394685448612144	26.52361812844129	24.580115161213097
44-45	23.032242619728603	25.543361850643215	26.43479027807527	24.989605251552913
46-47	23.262187055073266	25.28317814708884	26.74411279247042	24.71052200536747
48-49	23.11288066828783	25.10489246160243	27.037685688006352	24.744541182103394
50-51	22.697090730404323	25.30207768971991	26.990436831428678	25.01039474844709
52-53	22.661811584159665	25.41295500648884	27.22101125152771	24.70422215782378
54-55	22.251061524311112	25.406655158945153	27.257550367281112	25.084732949462623
56-57	21.81007219625285	25.418624869278162	27.38858719618985	25.38271573827913
58-59	22.64039210251112	25.305227613491756	26.957047639447122	25.09733264455
60-61	22.045056509632467	25.24222913805486	26.94696788337722	25.76574646893545
62-63	21.508939483664495	24.992755175324756	27.597112149885973	25.901193191124776
64-65	22.201292728715966	25.551551652450012	27.002406541761694	25.24474907707234
66-67	22.153413887383923	25.13828165358398	26.747262716242265	25.961041742789824
68-69	22.24539166152179	25.111822293900488	26.454319805460706	26.188466239117012
70-71	22.47281615784898	25.281918177580103	26.76301233510149	25.48225332946943
72-73	22.718510212052866	24.806279688031548	26.483299104161677	25.9919109957539
74-75	22.511245227865487	25.11056232439175	26.37620169591896	26.001990751823804
76	22.88482618720627	24.666423072561646	26.45179986644323	25.996950873788855
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	0.5
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	1.0
15	2.5
16	6.0
17	9.0
18	17.5
19	26.5
20	41.5
21	56.0
22	71.5
23	115.5
24	170.5
25	197.0
26	228.5
27	317.0
28	455.0
29	536.0
30	623.0
31	832.0
32	1167.5
33	1381.0
34	1489.5
35	1834.0
36	2315.0
37	2560.0
38	2782.0
39	3368.5
40	4025.0
41	4419.0
42	4521.0
43	4638.0
44	4724.0
45	4665.5
46	4638.0
47	4628.5
48	4346.0
49	3911.5
50	3750.0
51	3434.0
52	2736.5
53	2267.5
54	2180.0
55	1959.0
56	1645.5
57	1428.5
58	1304.0
59	1163.0
60	961.5
61	859.0
62	817.0
63	806.5
64	800.0
65	822.0
66	879.0
67	918.0
68	917.0
69	910.5
70	909.5
71	914.0
72	933.5
73	897.5
74	810.0
75	778.0
76	733.5
77	623.0
78	500.0
79	443.0
80	379.0
81	274.5
82	183.0
83	132.0
84	111.5
85	80.5
86	52.5
87	35.0
88	29.5
89	18.0
90	8.5
91	4.0
92	3.0
93	1.5
94	2.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	79367.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.93591795078559
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.16162846331173	42.966220217470735
2	13.237788699926059	15.338868799375055
3	4.597451176547344	7.990726624415689
4	2.3748423296072376	5.503546814167097
5	1.4766647818711671	4.277596482165132
6	0.956896176764821	3.326319503068026
7	0.6850506720020878	2.778232766767044
8	0.5436910095254665	2.5199390174757768
9	0.4175546953155583	2.1772273110990716
>10	1.5462572310904266	13.05706401905074
>50	0.002174764038101866	0.06425844494563232
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.002519939017475777	0.0
22	0.0	0.0	0.0	0.006299847543689442	0.0
23	0.0	0.0	0.0	0.006299847543689442	0.0
24	0.0	0.0	0.0	0.010079756069903108	0.0
25	0.0	0.0	0.0	0.010079756069903108	0.0
26	0.0	0.0	0.0	0.010079756069903108	0.0
27	0.0	0.0	0.0	0.020159512139806216	0.0
28	0.0	0.0	0.0	0.02519939017475777	0.0
29	0.0	0.0	0.0	0.03401917673592299	0.0
30	0.0	0.0	0.0	0.04661887182330188	0.0
31	0.0	0.0	0.0	0.05417868887572921	0.0
32	0.0	0.0	0.0	0.07055829248932176	0.0
33	0.0	0.0	0.0	0.09575768266407952	0.0
34	0.0	0.0	0.0	0.13481673743495406	0.0
35	0.0	0.0	0.0	0.19025539581942116	0.0
36	0.0	0.0	0.0	0.2734133833961218	0.0
37	0.0	0.0	0.0	0.4107500598485517	0.0
38	0.0	0.0	0.0	0.5758060654932151	0.0
39	0.0	0.0	0.0	0.7975606990310834	0.0
40	0.0	0.0	0.0	1.0268751496213793	0.0
41	0.0	0.0	0.0	1.2700492648077917	0.0
42	0.0	0.0	0.0	1.5359028311514862	0.0
43	0.0	0.0	0.0	1.8584550253883856	0.0
44	0.0	0.0	0.0	2.238965817027228	0.0
45	0.0	0.0	0.0	2.6635755414718965	0.0
46	0.0	0.0	0.0	3.14488389380977	0.0
47	0.0	0.0	0.0	3.661471392392304	0.0
48	0.0	0.0	0.0	4.185618708027266	0.0
49	0.0	0.0	0.0	4.731185505310772	0.0
50	0.0	0.0	0.0	5.17973465042146	0.0
51	0.0	0.0	0.0	5.606864313883604	0.0
52	0.0	0.0	0.0	6.0428137639069135	0.0
53	0.0	0.0	0.0	6.490102939508864	0.0
54	0.0	0.0	0.0	6.9814910479166405	0.0
55	0.0	0.0	0.0	7.501858455025388	0.0
56	0.0	0.0	0.0	8.057505008378797	0.0
57	0.0	0.0	0.0	8.577872415487546	0.0
58	0.0	0.0	0.0	9.146118663928332	0.0
59	0.0	0.0	0.0	9.590887900512808	0.0
60	0.0	0.0	0.0	10.122595033200197	0.0
61	0.0	0.0	0.0	10.640442501291469	0.0
62	0.0	0.0	0.0	11.184749329066237	0.0
63	0.0	0.0	0.0	11.700076858140033	0.0
64	0.0	0.0	0.0	12.201544722617713	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGGTTG	15	0.0022172583	70.0	31
TAATACA	25	2.3774494E-4	56.000004	4
TGGAGGC	20	0.0069283284	52.5	13
GCTGTCC	20	0.0069283284	52.5	8
TGTCCCC	20	0.0069283284	52.5	10
TATCTAT	35	0.0012487185	39.999996	35
CCAGTCA	35	0.0012487185	39.999996	14
GGCTTAA	45	0.004284564	31.111109	23
TGACAAC	45	0.004284564	31.111109	39
TTCTCAG	45	0.004284564	31.111109	56
CATACAC	60	4.94309E-4	29.166666	3
TATACCA	60	4.94309E-4	29.166666	10
ACTGATT	50	0.0071706166	28.000002	42
CCCTAGA	50	0.0071706166	28.000002	69
GTAATAC	50	0.0071706166	28.000002	3
GCCTACA	50	0.0071706166	28.000002	1
AATACCC	50	0.0071706166	28.000002	5
AGATGTG	50	0.0071706166	28.000002	5
ATACCCA	65	7.893867E-4	26.923079	6
CTATACT	80	8.571271E-5	26.25	4
>>END_MODULE