FastQCFastQC Report
Wed 25 May 2016
SRR1781136_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781136_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74796
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT6270.8382801219316541No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT1000.052.50000447
TAAACCA200.00692676352.55
ATAGTGA200.00692676352.56
GCATACG200.00692676352.51
TCTCGTA850.049.41176638
CTGCTTG1100.047.72727254
TCTTCTG1100.047.72727250
CGTCTTC1100.047.72727248
TTCTGCT1100.047.72727252
TGCCGTC1100.047.72727245
ATCTCGT900.046.66666837
TATGCCG1050.046.66666443
TCGTATG1050.046.66666440
ATGCCTC305.844003E-446.66666467
CTCGTAT1000.045.50000439
GTATGCC1100.044.54545242
GCCGTCT1200.043.7546
CGTATGC1150.042.60869641
TTGAAAA1150.042.60869658
CTTGAAA1250.042.057
Produced by FastQC (version 0.11.5)