##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781136_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	74796
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.94657468313814	34.0	34.0	34.0	31.0	34.0
2	32.95585325418472	34.0	34.0	34.0	31.0	34.0
3	33.05588534146211	34.0	34.0	34.0	31.0	34.0
4	33.1834322691053	34.0	34.0	34.0	32.0	34.0
5	33.1454088453928	34.0	34.0	34.0	32.0	34.0
6	36.28294293812503	38.0	37.0	38.0	34.0	38.0
7	36.14855072463768	38.0	36.0	38.0	32.0	38.0
8	35.83881490988823	38.0	36.0	38.0	31.0	38.0
9	35.86274667094497	38.0	36.0	38.0	31.0	38.0
10-11	35.75093587892401	38.0	36.0	38.0	31.0	38.0
12-13	35.69874725921172	38.0	36.0	38.0	31.0	38.0
14-15	35.70204288999412	38.0	36.0	38.0	31.0	38.0
16-17	35.635488528798334	38.0	36.0	38.0	31.0	38.0
18-19	35.63528798331461	38.0	36.0	38.0	31.0	38.0
20-21	35.55275683191614	38.0	36.0	38.0	31.0	38.0
22-23	35.62056794480988	38.0	36.0	38.0	31.0	38.0
24-25	35.59519894111985	38.0	36.0	38.0	31.0	38.0
26-27	35.34929006898765	38.0	36.0	38.0	31.0	38.0
28-29	35.28047622867533	38.0	36.0	38.0	31.0	38.0
30-31	35.31966950104284	38.0	36.0	38.0	31.0	38.0
32-33	35.23359537943205	38.0	35.5	38.0	30.0	38.0
34-35	35.25362987325526	38.0	35.5	38.0	30.0	38.0
36-37	35.21121450344938	37.5	35.0	38.0	29.0	38.0
38-39	35.162034065992835	37.5	35.0	38.0	27.5	38.0
40-41	35.20741082410824	37.0	35.0	38.0	28.5	38.0
42-43	35.18049093534414	37.0	35.0	38.0	28.0	38.0
44-45	35.138316220118725	37.0	35.0	38.0	27.0	38.0
46-47	35.18212872346115	37.0	35.0	38.0	27.5	38.0
48-49	35.14374431787796	37.0	35.0	38.0	27.0	38.0
50-51	35.05109898925076	37.0	35.0	38.0	27.0	38.0
52-53	35.06943553131184	37.0	35.0	38.0	27.0	38.0
54-55	35.09787288090272	37.0	35.0	38.0	27.0	38.0
56-57	35.01737392373924	37.0	35.0	38.0	27.0	38.0
58-59	35.01147120166854	37.0	35.0	38.0	27.0	38.0
60-61	35.00411118241617	37.0	35.0	38.0	27.0	38.0
62-63	34.965111770682924	37.0	35.0	38.0	27.0	38.0
64-65	34.90845767153324	37.0	35.0	38.0	27.0	38.0
66-67	34.89284186320124	37.0	35.0	38.0	27.0	38.0
68-69	34.81138697256538	37.0	35.0	38.0	27.0	38.0
70-71	34.725546820685594	37.0	34.0	38.0	27.0	38.0
72-73	34.700438526124394	37.0	34.5	38.0	27.0	38.0
74-75	34.70837344243007	37.0	34.5	38.0	27.0	38.0
76	33.66364511471202	37.0	34.0	38.0	24.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	1.0
21	6.0
22	22.0
23	40.0
24	121.0
25	182.0
26	405.0
27	683.0
28	1070.0
29	1657.0
30	2401.0
31	3493.0
32	4575.0
33	6004.0
34	7829.0
35	9803.0
36	13779.0
37	22722.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.12685170329964	18.241617198780684	9.015187977966736	25.61634311995294
2	24.399700518744318	20.851382426867744	30.297074709877535	24.451842344510403
3	21.014492753623188	22.110808064602384	28.84913631745013	28.025562864324293
4	14.673244558532542	17.256270388790846	35.75191186694476	32.31857318573186
5	14.447296646879511	34.414941975506714	35.31739665222739	15.820364725386385
6	31.329215466067705	37.57553879886625	17.356543130648696	13.73870260441735
7	30.57115353762233	28.952082999090862	20.918230921439648	19.558532541847157
8	26.256751697951763	35.45644152093695	18.36729236857586	19.91951441253543
9	25.5588534146211	15.641210759933685	19.60666345793893	39.19327236750628
10-11	26.11570137440505	24.45451628429328	26.935263917856574	22.494518423445104
12-13	25.911144981015028	22.219771110754586	26.06155409380181	25.80752981442858
14-15	24.033370768490293	22.92101181881384	22.862853628536286	30.182763784159583
16-17	25.1898497245842	26.837665115781594	24.011979250227284	23.96050590940692
18-19	24.641023584148886	25.887748007914862	24.834884218407403	24.636344189528852
20-21	23.5908337344243	26.091635916359163	25.199876998769987	25.11765335044655
22-23	24.08150168458206	26.008743783090004	25.58091341782983	24.3288411144981
24-25	24.539413872399592	26.084951066901972	25.241991550350285	24.133643510348147
26-27	23.676399807476336	26.479357184876196	25.600299481255682	24.24394352639179
28-29	24.40504839831007	25.801513449917106	26.12706561848227	23.66637253329055
30-31	24.136317450131024	26.46732445585325	25.644419487673137	23.751938606342584
32-33	23.384940371142843	26.925905128616506	26.092972886250603	23.59618161399005
34-35	24.922455746296592	25.239986095513128	25.748034654259587	24.08952350393069
36-37	24.366944756404084	25.637734638215946	25.2038879084443	24.791432696935665
38-39	23.637627680624632	26.008075298144284	26.247392908711696	24.106904112519388
40-41	23.84418952885181	24.87766725493342	26.870420878121827	24.407722338092945
42-43	24.352906572543986	25.46994491684047	26.228675330231567	23.948473180383978
44-45	23.111530028343765	26.205278357131395	26.759452377132465	23.923739237392375
46-47	24.179100486657042	25.130354564415207	26.150462591582436	24.540082357345312
48-49	23.711161024653723	24.989304240868496	26.36103534948393	24.93849938499385
50-51	22.95376758115407	24.736616931386703	27.325659126156477	24.98395636130274
52-53	22.81138028771592	24.822183004438738	27.91258890849778	24.453847799347557
54-55	22.23648323439756	25.138376383763838	27.434622172308682	25.190518209529923
56-57	22.537969944916842	25.16778972137547	26.967351195251084	25.326889138456604
58-59	22.618856623348844	25.024733942991606	27.55160703780951	24.804802395850047
60-61	22.841462110273277	25.42315097064014	26.63511417722873	25.100272741857854
62-63	21.304615220065244	26.254077758168886	26.818947537301458	25.622359484464408
64-65	21.969089256109953	26.49540082357345	26.277474731269052	25.258035189047547
66-67	22.070030482913523	26.151799561473876	25.75806192844537	26.020108027167225
68-69	21.79996256484304	26.540857799882346	25.895101342317773	25.764078292956842
70-71	22.48248569442216	26.04417348521311	25.800176480025673	25.673164340339056
72-73	22.065351088293493	25.60297342103856	25.49334188994064	26.83833360072731
74-75	22.002513503395903	25.825578907962992	25.63840312316167	26.533504465479435
76	22.450398417027646	25.655115246804645	25.374351569602656	26.520134766565057
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.0
10	1.0
11	0.0
12	0.0
13	0.0
14	0.5
15	7.5
16	15.5
17	17.0
18	18.5
19	24.0
20	43.0
21	58.0
22	74.0
23	106.5
24	142.0
25	161.0
26	223.0
27	336.5
28	470.5
29	553.0
30	615.5
31	762.5
32	1046.0
33	1245.0
34	1353.0
35	1624.5
36	2045.5
37	2303.0
38	2579.0
39	3105.0
40	3649.0
41	4181.0
42	4419.0
43	4511.0
44	4643.0
45	4600.5
46	4518.0
47	4481.0
48	4141.5
49	3577.0
50	3315.0
51	3086.0
52	2559.0
53	2129.0
54	1997.0
55	1868.5
56	1552.0
57	1253.0
58	1142.0
59	1043.0
60	910.5
61	832.5
62	788.0
63	799.0
64	779.5
65	795.0
66	868.5
67	896.0
68	927.5
69	944.0
70	925.5
71	922.0
72	901.5
73	845.5
74	760.0
75	710.0
76	667.0
77	571.5
78	422.5
79	326.0
80	311.0
81	246.5
82	159.0
83	121.0
84	101.5
85	76.0
86	46.5
87	23.0
88	20.5
89	11.0
90	2.5
91	1.0
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	74796.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.37210546018504
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.4388456252863	43.451521471736456
2	13.433348602840129	15.682656826568268
3	4.491525423728813	7.865393871330017
4	2.402656894182318	5.609925664474035
5	1.2849289967934037	3.750200545483716
6	0.9344938158497481	3.272902294240334
7	0.7214841960604673	2.9480186106208888
8	0.46266605588639487	2.16054334456388
9	0.3939532753092075	2.0696293919460933
>10	1.429225836005497	12.199850259372159
>50	0.004580852038479157	0.15107759773249907
>100	0.0	0.0
>500	0.0022904260192395786	0.8382801219316541
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT	627	0.8382801219316541	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0013369698914380448	0.0
15	0.0	0.0	0.0	0.0013369698914380448	0.0
16	0.0	0.0	0.0	0.0013369698914380448	0.0
17	0.0	0.0	0.0	0.0013369698914380448	0.0
18	0.0	0.0	0.0	0.0013369698914380448	0.0
19	0.0	0.0	0.0	0.0013369698914380448	0.0
20	0.0	0.0	0.0	0.0013369698914380448	0.0
21	0.0	0.0	0.0	0.0026739397828760897	0.0
22	0.0	0.0	0.0	0.0026739397828760897	0.0
23	0.0	0.0	0.0	0.004010909674314134	0.0
24	0.0	0.0	0.0	0.005347879565752179	0.0
25	0.0	0.0	0.0	0.005347879565752179	0.0
26	0.0	0.0	0.0	0.005347879565752179	0.0
27	0.0	0.0	0.0	0.013369698914380447	0.0
28	0.0	0.0	0.0	0.020054548371570672	0.0
29	0.0	0.0	0.0	0.025402427937322853	0.0
30	0.0	0.0	0.0	0.03609818706882721	0.0
31	0.0	0.0	0.0	0.042783036526017434	0.0
32	0.0	0.0	0.0	0.05748970533183593	0.0
33	0.0	0.0	0.0	0.08155516337772073	0.0
34	0.0	0.0	0.0	0.1163163805551099	0.0
35	0.0	0.0	0.0	0.1724691159955078	0.0
36	0.0	0.0	0.0	0.24867639980747633	0.0
37	0.0	0.0	0.0	0.3970800577570993	0.0
38	0.0	0.0	0.0	0.5642012941868549	0.0
39	0.0	0.0	0.0	0.7781164768169421	0.0
40	0.0	0.0	0.0	0.9987165089042195	0.0
41	0.0	0.0	0.0	1.2380341194716296	0.0
42	0.0	0.0	0.0	1.5027541579763624	0.0
43	0.0	0.0	0.0	1.8062463233327986	0.0
44	0.0	0.0	0.0	2.1645542542381944	0.0
45	0.0	0.0	0.0	2.587036739932617	0.0
46	0.0	0.0	0.0	3.0576501417188084	0.0
47	0.0	0.0	0.0	3.573720519813894	0.0
48	0.0	0.0	0.0	4.088453928017541	0.0
49	0.0	0.0	0.0	4.63661158350714	0.0
50	0.0	0.0	0.0	5.076474677790256	0.0
51	0.0	0.0	0.0	5.500294133376117	0.0
52	0.0	0.0	0.0	5.934809348093481	0.0
53	0.0	0.0	0.0	6.3760094122680355	0.0
54	0.0	0.0	0.0	6.847959783945665	0.0
55	0.0	0.0	0.0	7.362693192149313	0.0
56	0.0	0.0	0.0	7.898818118615969	0.0
57	0.0	0.0	0.0	8.449649713888443	0.0
58	0.0	0.0	0.0	8.997807369378041	0.0
59	0.0	0.0	0.0	9.467083801272794	0.0
60	0.0	0.0	0.0	9.981817209476443	0.0
61	0.0	0.0	0.0	10.499224557462966	0.0
62	0.0	0.0	0.0	11.032675544146747	0.0
63	0.0	0.0	0.0	11.528691373870261	0.0
64	0.0	0.0	0.0	12.022033263810899	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTCTT	100	0.0	52.500004	47
TAAACCA	20	0.006926763	52.5	5
ATAGTGA	20	0.006926763	52.5	6
GCATACG	20	0.006926763	52.5	1
TCTCGTA	85	0.0	49.411766	38
CTGCTTG	110	0.0	47.727272	54
TCTTCTG	110	0.0	47.727272	50
CGTCTTC	110	0.0	47.727272	48
TTCTGCT	110	0.0	47.727272	52
TGCCGTC	110	0.0	47.727272	45
ATCTCGT	90	0.0	46.666668	37
TATGCCG	105	0.0	46.666664	43
TCGTATG	105	0.0	46.666664	40
ATGCCTC	30	5.844003E-4	46.666664	67
CTCGTAT	100	0.0	45.500004	39
GTATGCC	110	0.0	44.545452	42
GCCGTCT	120	0.0	43.75	46
CGTATGC	115	0.0	42.608696	41
TTGAAAA	115	0.0	42.608696	58
CTTGAAA	125	0.0	42.0	57
>>END_MODULE