FastQCFastQC Report
Wed 25 May 2016
SRR1781135_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781135_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences251842
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGATT200.006945765652.546
TTCCCGT450.004302135731.11111345
ACCCTTA604.973377E-429.1666666
GGGAATC500.00719984827.99999824
TACACTG1301.5972728E-724.2307685
GGTTTAG750.00182907723.33333438
GTTCGAG901.9230583E-423.33333456
GGTATAG750.00182907723.3333341
GAGTTCG750.00182907723.33333454
TTACACT1052.0324402E-523.3333324
ATAATAC1854.0017767E-1022.7027033
AATACTC952.7730592E-422.1052635
TTTACAC1355.9939375E-620.740743
CTCCGTC850.003782669820.5882369
AGTTCGA850.003782669820.58823655
GTATAAG1408.209308E-620.0000021
CCTAGAC1055.450295E-420.03
CTATACT1055.450295E-420.04
GGCCTGA1055.450295E-420.06
GACTCCG1055.450295E-420.07
Produced by FastQC (version 0.11.5)