##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781135_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	251842
Sequences flagged as poor quality	0
Sequence length	76
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.49119686152429	34.0	34.0	34.0	31.0	34.0
2	32.68436956504475	34.0	34.0	34.0	31.0	34.0
3	32.6953724954535	34.0	34.0	34.0	31.0	34.0
4	32.79632070901597	34.0	34.0	34.0	31.0	34.0
5	32.788684969147326	34.0	34.0	34.0	31.0	34.0
6	35.97197846268692	38.0	37.0	38.0	32.0	38.0
7	35.887214999880875	38.0	37.0	38.0	32.0	38.0
8	35.947208964350665	38.0	37.0	38.0	32.0	38.0
9	36.1030765321114	38.0	37.0	38.0	34.0	38.0
10-11	36.0559040986015	38.0	37.0	38.0	34.0	38.0
12-13	36.002227587137966	38.0	37.0	38.0	33.0	38.0
14-15	35.95119162014279	38.0	37.0	38.0	32.0	38.0
16-17	35.945743759976494	38.0	37.0	38.0	32.5	38.0
18-19	35.979074181431216	38.0	37.0	38.0	33.0	38.0
20-21	35.95941503005853	38.0	37.0	38.0	32.5	38.0
22-23	35.98497073561995	38.0	37.0	38.0	33.0	38.0
24-25	36.00188014707634	38.0	37.0	38.0	33.0	38.0
26-27	35.949764137832446	38.0	37.0	38.0	32.0	38.0
28-29	36.02830941622128	38.0	37.0	38.0	33.5	38.0
30-31	36.08343127834118	38.0	37.0	38.0	34.0	38.0
32-33	36.054669197353896	38.0	37.0	38.0	33.5	38.0
34-35	36.077645904972165	38.0	37.0	38.0	34.0	38.0
36-37	35.985101770157485	38.0	37.0	38.0	33.0	38.0
38-39	36.005934276252574	38.0	37.0	38.0	33.0	38.0
40-41	35.99188380016042	38.0	37.0	38.0	33.0	38.0
42-43	36.066033465426734	38.0	37.0	38.0	34.0	38.0
44-45	36.06653179374369	38.0	37.0	38.0	34.0	38.0
46-47	36.042731156836425	38.0	37.0	38.0	33.5	38.0
48-49	36.014507111601716	38.0	37.0	38.0	33.5	38.0
50-51	35.950599979352134	38.0	37.0	38.0	32.5	38.0
52-53	35.99496708253588	38.0	37.0	38.0	33.5	38.0
54-55	35.96704481381184	38.0	37.0	38.0	32.5	38.0
56-57	35.912403411662865	38.0	37.0	38.0	32.0	38.0
58-59	35.91333653639981	38.0	37.0	38.0	32.0	38.0
60-61	35.922234972720986	38.0	37.0	38.0	32.0	38.0
62-63	35.89424123061285	38.0	37.0	38.0	32.0	38.0
64-65	35.90879996188086	38.0	37.0	38.0	32.0	38.0
66-67	35.83093169526926	38.0	37.0	38.0	32.0	38.0
68-69	35.595095337552905	38.0	37.0	38.0	31.0	38.0
70-71	35.78552822801598	38.0	37.0	38.0	31.5	38.0
72-73	35.79873889184489	38.0	37.0	38.0	31.5	38.0
74-75	35.827340157717934	38.0	37.0	38.0	32.0	38.0
76	34.884864319692504	37.0	35.0	38.0	27.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	20.0
14	43.0
15	153.0
16	264.0
17	395.0
18	514.0
19	600.0
20	666.0
21	707.0
22	788.0
23	916.0
24	1142.0
25	1450.0
26	1676.0
27	2126.0
28	2590.0
29	3187.0
30	3889.0
31	5090.0
32	6575.0
33	8914.0
34	13233.0
35	20751.0
36	39901.0
37	136250.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.991931449083154	20.159067987071257	9.286377967138126	24.56262259670746
2	20.453697159330055	23.15062618625964	33.72312799294796	22.672548661462347
3	18.74032131256899	23.974158400902155	32.16064040152159	25.124879885007267
4	12.763955178246677	17.746841273496873	39.235314204938014	30.25388934331843
5	12.246170217834992	34.59470620468389	37.76852153334234	15.390602044138785
6	27.118590227205946	39.70822976310544	20.01294462401029	13.160235385678323
7	25.228913366316974	32.46321106090326	23.97257010347758	18.33530546930218
8	22.888557111204644	37.7871840280811	21.824000762382763	17.500258098331496
9	23.40951866646548	17.60389450528506	23.163332565656244	35.82325426259321
10-11	22.928066009640965	26.071505150054396	30.052572644753457	20.94785619555118
12-13	23.44565243287458	24.461964247424973	29.389855544349235	22.70252777535121
14-15	21.393373623144672	25.948213562471707	26.382017296558953	26.276395517824668
16-17	20.971680656919816	29.654505602719166	26.892257844203904	22.481555896157115
18-19	20.230144296821024	29.34220662161196	28.72237355167129	21.70527552989573
20-21	20.811262617037666	29.256835635041018	28.625487408772166	21.30641433914915
22-23	20.43682149919394	29.15180946784095	29.037253516093426	21.37411551687169
24-25	20.93058346105892	28.98444262672628	28.629259615155533	21.455714297059266
26-27	20.59029073784357	28.72952089008188	28.67571731482437	22.00447105725018
28-29	20.329611423035075	28.894703822237748	29.01303198036864	21.762652774358525
30-31	20.51484661017622	29.40017947760898	28.75731609501195	21.327657817202848
32-33	20.084219470938127	29.364045711199882	29.056710159544473	21.495024658317515
34-35	20.384010609826795	28.880806219772715	29.402164849389695	21.333018321010794
36-37	20.627417190143028	29.404150221170415	28.860555427609373	21.107877161077184
38-39	20.490029462917224	29.410106336512577	28.700335924905296	21.3995282756649
40-41	20.093749255485584	29.17662661509994	29.261997601670892	21.467626527743587
42-43	19.994877740805745	29.094233686200077	29.432541037634707	21.47834753535947
44-45	20.20671690980853	29.018193946998515	29.467483580975372	21.30760556221758
46-47	20.30161768092693	29.356898372789285	29.227253595508294	21.114230350775486
48-49	20.09494047855401	29.08450536447455	29.3298973165715	21.490656840399932
50-51	20.249600940272074	28.769426862874344	29.488925596207142	21.492046600646436
52-53	19.97839915502577	28.797619142160563	29.7357073085506	21.488274394263073
54-55	20.121345923237584	28.990200204890364	29.641799223322558	21.246654648549487
56-57	20.016319756037515	28.961015239713788	29.614202555570557	21.40846244867814
58-59	19.87754226856521	28.551433041351327	29.853042780791135	21.717981909292334
60-61	19.93988294247981	28.860753964787445	29.432541037634707	21.766822055098036
62-63	19.838231907306962	28.847054899500478	29.574693657134233	21.740019536058323
64-65	19.726455476052447	28.470826947054107	29.9679958068948	21.834721769998648
66-67	19.727249624764735	28.462488385575085	29.597723969790586	22.2125380198696
68-69	20.066946736445868	28.13073276101683	29.533794998451413	22.268525504085897
70-71	20.295860102762845	28.420597041001898	29.35392031511821	21.92962254111705
72-73	19.98852455110744	28.53396176968099	29.41447415443016	22.06303952478141
74-75	19.91288188626202	28.491077739217445	29.364641322734098	22.23139905178644
76	20.079256041486328	28.814494802296682	28.993178262561447	22.113070893655546
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	1.0
6	1.5
7	1.5
8	1.0
9	4.5
10	5.5
11	7.5
12	12.0
13	22.5
14	52.0
15	109.5
16	206.0
17	264.0
18	345.5
19	589.5
20	961.5
21	1171.0
22	1436.0
23	2087.5
24	2933.0
25	3392.0
26	3797.5
27	4796.0
28	5982.5
29	6576.0
30	7143.5
31	8159.5
32	9175.5
33	9743.0
34	10185.5
35	11023.0
36	11760.5
37	12103.0
38	12199.5
39	12404.0
40	12517.5
41	12305.0
42	12087.0
43	11854.5
44	11356.0
45	10700.0
46	10310.0
47	9992.0
48	9280.0
49	8435.0
50	7984.0
51	7612.0
52	6888.0
53	6350.5
54	6165.0
55	5934.0
56	5472.5
57	4927.0
58	4612.0
59	4200.5
60	3473.5
61	2831.0
62	2504.0
63	2185.5
64	1686.0
65	1352.5
66	1119.5
67	1039.0
68	957.5
69	830.5
70	732.0
71	679.0
72	611.5
73	539.0
74	497.5
75	461.0
76	401.0
77	344.0
78	305.5
79	264.0
80	221.5
81	164.0
82	119.5
83	90.0
84	85.0
85	59.0
86	37.5
87	37.0
88	27.0
89	13.0
90	8.5
91	6.0
92	4.0
93	5.0
94	3.0
95	0.5
96	1.0
97	0.5
98	0.0
99	4.5
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	251842.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.19557312096055
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.52203581791399	67.82946029161874
2	14.250343667191453	23.42630329790904
3	2.574336468489245	6.347971843009892
4	0.4864018511181709	1.5992031567901674
5	0.10460819415037222	0.42991652356692783
6	0.030971273930229856	0.15274209665889044
7	0.014770595639995079	0.08498543017771495
8	0.005372258791893095	0.03532607122830176
9	0.005347931909598496	0.03956186954891892
>10	0.005811940865066254	0.05452941949142136
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0011912230684317945	0.0
6	0.0	0.0	0.0	0.0011912230684317945	0.0
7	0.0	0.0	0.0	0.0011912230684317945	0.0
8	0.0	0.0	0.0	0.0011912230684317945	0.0
9	0.0	0.0	0.0	0.0011912230684317945	0.0
10	0.0	0.0	0.0	0.0011912230684317945	0.0
11	0.0	0.0	0.0	0.0019853717807196574	0.0
12	0.0	0.0	0.0	0.0019853717807196574	0.0
13	0.0	0.0	0.0	0.0027795204930075204	0.0
14	0.0	0.0	0.0	0.003573669205295384	0.0
15	0.0	0.0	0.0	0.004764892273727178	0.0
16	0.0	0.0	0.0	0.004764892273727178	0.0
17	0.0	0.0	0.0	0.004764892273727178	0.0
18	0.0	0.0	0.0	0.004764892273727178	0.0
19	0.0	0.0	0.0	0.004764892273727178	0.0
20	0.0	0.0	0.0	0.005956115342158973	0.0
21	0.0	0.0	0.0	0.005956115342158973	0.0
22	0.0	0.0	0.0	0.008735635835166494	0.0
23	0.0	0.0	0.0	0.008735635835166494	0.0
24	0.0	0.0	0.0	0.009132710191310425	0.0
25	0.0	0.0	0.0	0.011118081972030082	0.0
26	0.0	0.0	0.0	0.011515156328174014	0.0
27	0.0	0.0	0.0	0.012309305040461876	0.0
28	0.0	0.0	0.0	0.015088825533469397	0.0
29	0.0	0.0	0.0	0.02064786651948444	0.0
30	0.0	0.0	0.0	0.024618610080923753	0.0
31	0.0	0.0	0.0	0.029383502354650932	0.0
32	0.0	0.0	0.0	0.035339617696809905	0.0
33	0.0	0.0	0.0	0.0428840304635446	0.0
34	0.0	0.0	0.0	0.05201674065485503	0.0
35	0.0	0.0	0.0	0.062340673914597244	0.0
36	0.0	0.0	0.0	0.07941487122878631	0.0
37	0.0	0.0	0.0	0.12150475298004304	0.0
38	0.0	0.0	0.0	0.19972840114039755	0.0
39	0.0	0.0	0.0	0.29423209790265326	0.0
40	0.0	0.0	0.0	0.41891344573184774	0.0
41	0.0	0.0	0.0	0.5455801653417619	0.0
42	0.0	0.0	0.0	0.6595405055550703	0.0
43	0.0	0.0	0.0	0.7818394072474012	0.0
44	0.0	0.0	0.0	0.9077119781450275	0.0
45	0.0	0.0	0.0	1.0391435900286687	0.0
46	0.0	0.0	0.0	1.1908259940756507	0.0
47	0.0	3.9707435614393154E-4	0.0	1.3651416364228366	0.0
48	0.0	3.9707435614393154E-4	0.0	1.6014008783284759	0.0
49	0.0	3.9707435614393154E-4	0.0	1.8682348456571978	0.0
50	0.0	3.9707435614393154E-4	0.0	2.13506881298592	0.0
51	0.0	3.9707435614393154E-4	0.0	2.388402252205748	0.0
52	0.0	3.9707435614393154E-4	0.0	2.6274410146043947	0.0
53	0.0	3.9707435614393154E-4	0.0	2.8394787207852543	0.0
54	0.0	3.9707435614393154E-4	0.0	3.0515164269661135	0.0
55	0.0	3.9707435614393154E-4	0.0	3.270701471557564	0.0
56	0.0	3.9707435614393154E-4	0.0	3.485518698231431	0.0
57	0.0	3.9707435614393154E-4	0.0	3.705497891535169	0.0
58	0.0	3.9707435614393154E-4	0.0	3.9572430333304216	0.0
59	0.0	3.9707435614393154E-4	0.0	4.229636041645159	0.0
60	0.0	3.9707435614393154E-4	0.0	4.514735429356501	0.0
61	0.0	3.9707435614393154E-4	0.0	4.822468055368049	0.0
62	0.0	3.9707435614393154E-4	0.0	5.120273822475997	0.0
63	0.0	3.9707435614393154E-4	0.0	5.387107789804719	0.0
64	0.0	3.9707435614393154E-4	0.0	5.6178079907243434	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGATT	20	0.0069457656	52.5	46
TTCCCGT	45	0.0043021357	31.111113	45
ACCCTTA	60	4.973377E-4	29.166666	6
GGGAATC	50	0.007199848	27.999998	24
TACACTG	130	1.5972728E-7	24.230768	5
GGTTTAG	75	0.001829077	23.333334	38
GTTCGAG	90	1.9230583E-4	23.333334	56
GGTATAG	75	0.001829077	23.333334	1
GAGTTCG	75	0.001829077	23.333334	54
TTACACT	105	2.0324402E-5	23.333332	4
ATAATAC	185	4.0017767E-10	22.702703	3
AATACTC	95	2.7730592E-4	22.105263	5
TTTACAC	135	5.9939375E-6	20.74074	3
CTCCGTC	85	0.0037826698	20.588236	9
AGTTCGA	85	0.0037826698	20.588236	55
GTATAAG	140	8.209308E-6	20.000002	1
CCTAGAC	105	5.450295E-4	20.0	3
CTATACT	105	5.450295E-4	20.0	4
GGCCTGA	105	5.450295E-4	20.0	6
GACTCCG	105	5.450295E-4	20.0	7
>>END_MODULE