FastQCFastQC Report
Wed 25 May 2016
SRR1781135_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781135_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences237400
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT5730.24136478517270432No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT1000.052.49999644
TATGCCG950.051.5789543
CGTATGC1050.050.00000441
TCGTATG1050.050.00000440
GCCGTCT1000.048.99999646
CGTCTTC950.047.8947448
ATCTCGT1000.045.49999637
CCGTCTT1000.045.49999647
CTCGTAT1100.044.54545239
TCTCGTA1050.043.33333638
TGCCGTC1200.040.83333645
AGCGTCA350.001253757540.03
GCGTCAG350.001253757540.04
TATCTCG1200.037.91666836
GCGCGGC400.002415581435.031
CCGCGCG400.002415581435.029
GTATGCC1550.031.61290442
CGCGCGG450.004301645331.11110930
CTGCTTG1800.029.16666454
GGAGACG500.007199030427.9999986