##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781135_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	237400
Sequences flagged as poor quality	0
Sequence length	76
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.885126368997476	34.0	34.0	34.0	31.0	34.0
2	32.98354254422915	34.0	34.0	34.0	31.0	34.0
3	33.09277590564448	34.0	34.0	34.0	31.0	34.0
4	33.198917438921654	34.0	34.0	34.0	32.0	34.0
5	33.162228306655436	34.0	34.0	34.0	32.0	34.0
6	36.24848357203033	38.0	37.0	38.0	34.0	38.0
7	36.137712721145746	38.0	37.0	38.0	32.0	38.0
8	35.77656697556866	38.0	36.0	38.0	31.0	38.0
9	35.826107834877845	38.0	36.0	38.0	31.0	38.0
10-11	35.79565290648694	38.0	36.0	38.0	31.0	38.0
12-13	35.733430918281385	38.0	36.0	38.0	31.0	38.0
14-15	35.75532434709351	38.0	36.0	38.0	31.0	38.0
16-17	35.66993049705139	38.0	36.0	38.0	31.0	38.0
18-19	35.69411541701769	38.0	36.0	38.0	31.0	38.0
20-21	35.62417649536647	38.0	36.0	38.0	31.0	38.0
22-23	35.67057708508845	38.0	36.0	38.0	31.0	38.0
24-25	35.66403748946925	38.0	36.0	38.0	31.0	38.0
26-27	35.405524431339515	38.0	36.0	38.0	31.0	38.0
28-29	35.35040227464195	38.0	36.0	38.0	31.0	38.0
30-31	35.39999368155013	38.0	36.0	38.0	31.0	38.0
32-33	35.33437657961247	38.0	36.0	38.0	31.0	38.0
34-35	35.35037910699242	38.0	36.0	38.0	31.0	38.0
36-37	35.31474304970514	38.0	36.0	38.0	30.5	38.0
38-39	35.344264953664705	38.0	36.0	38.0	31.0	38.0
40-41	35.321286857624266	38.0	36.0	38.0	31.0	38.0
42-43	35.29739679865206	38.0	36.0	38.0	30.5	38.0
44-45	35.26101937657961	37.5	36.0	38.0	29.0	38.0
46-47	35.27627422072452	37.5	36.0	38.0	30.0	38.0
48-49	35.271278433024435	37.0	36.0	38.0	29.5	38.0
50-51	35.17308129738838	37.0	35.5	38.0	27.0	38.0
52-53	35.192173546756536	37.0	36.0	38.0	27.0	38.0
54-55	35.21687447346251	37.0	36.0	38.0	27.0	38.0
56-57	35.1222451558551	37.0	35.0	38.0	27.0	38.0
58-59	35.1353601516428	37.0	35.0	38.0	27.0	38.0
60-61	35.15653748946925	37.0	35.0	38.0	27.0	38.0
62-63	35.090149536647004	37.0	35.0	38.0	27.0	38.0
64-65	35.03763268744734	37.0	35.0	38.0	27.0	38.0
66-67	35.06513479359731	37.0	35.0	38.0	27.0	38.0
68-69	34.9812552653749	37.0	35.0	38.0	27.0	38.0
70-71	35.00256318449874	37.0	35.0	38.0	27.0	38.0
72-73	34.99121103622578	37.0	35.0	38.0	27.0	38.0
74-75	34.99453032855939	37.0	35.0	38.0	27.0	38.0
76	34.12040438079191	37.0	34.0	38.0	24.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	7.0
21	18.0
22	51.0
23	131.0
24	271.0
25	570.0
26	1024.0
27	1768.0
28	2980.0
29	4751.0
30	6970.0
31	10197.0
32	14085.0
33	18874.0
34	24698.0
35	32051.0
36	45885.0
37	73068.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.38247683235046	20.10909856781803	9.41491154170177	24.09351305812974
2	21.391322662173547	24.451979780960404	32.38921651221567	21.76748104465038
3	19.906908171861836	25.386689132266216	31.447346251053077	23.25905644481887
4	13.67228306655434	18.950294860994102	38.97051390058972	28.40690817186184
5	12.855096882898062	35.765374894692506	36.84035383319292	14.53917438921651
6	27.933445661331085	39.90732940185342	19.922072451558552	12.23715248525695
7	25.682813816343725	33.3921651221567	23.252737994945242	17.67228306655434
8	23.11541701769166	38.63100252737995	21.331929233361414	16.921651221566975
9	24.06866048862679	18.27632687447346	22.66301600673968	34.99199663016007
10-11	23.090143218197134	26.838247683235046	29.48989048020219	20.581718618365628
12-13	23.714827295703454	25.072662173546757	29.03875315922494	22.173757371524854
14-15	21.76263689974726	26.072662173546757	26.236310025273802	25.92839090143218
16-17	21.37636899747262	30.053706823925864	26.64174389216512	21.928180286436394
18-19	20.733150800336983	29.435551811288963	28.637110362257793	21.19418702611626
20-21	21.144481887110363	29.151642796967142	28.626579612468404	21.077295703454084
22-23	20.936394271272114	29.46419545071609	28.56718618365628	21.032224094355517
24-25	21.279907329401855	29.25	28.511162594776746	20.9589300758214
26-27	21.1969250210615	28.99536647009267	28.395324347093513	21.412384161752318
28-29	20.777379949452403	29.075610783487782	28.66385846672283	21.483150800336983
30-31	20.90016849199663	29.57055602358888	28.721988205560233	20.807287278854254
32-33	20.47514743049705	29.584456613310866	29.295914069081718	20.644481887110363
34-35	20.566133108677338	29.230202190395953	29.5787700084246	20.624894692502103
36-37	20.919755686604883	29.37594776748104	29.050126368997475	20.654170176916598
38-39	20.898904802021903	29.251263689974728	29.094144903117105	20.755686604886268
40-41	20.60425442291491	28.9589300758214	29.53306655433867	20.90374894692502
42-43	20.640690817186183	28.78306655433867	29.630160067396798	20.94608256107835
44-45	20.676495366470093	28.913016006739678	29.657961246840774	20.752527379949452
46-47	20.90459140690817	28.838458298230833	29.57413647851727	20.682813816343725
48-49	20.68871103622578	28.756739679865206	29.358466722830666	21.19608256107835
50-51	20.665543386689134	28.583192923336142	29.79317607413648	20.95808761583825
52-53	20.379738837405224	28.575610783487786	30.070977253580455	20.97367312552654
54-55	20.418281381634372	28.74157540016849	29.83129738837405	21.008845829823084
56-57	20.575400168491996	28.555602358887956	29.5821398483572	21.286857624262847
58-59	20.17312552653749	28.335509688289807	30.209140690817186	21.282224094355517
60-61	20.4863100252738	28.87173546756529	29.519587194608256	21.122367312552655
62-63	20.032645324347094	29.164279696714406	29.442080876158382	21.36099410278012
64-65	19.920808761583825	28.73146588037068	29.73188711036226	21.615838247683236
66-67	19.937026116259478	28.830244313395113	29.336352148272958	21.89637742207245
68-69	20.297177759056446	28.844987363100255	29.349831508003373	21.50800336983993
70-71	20.359519797809604	28.604675652906487	29.1695450716091	21.86625947767481
72-73	20.02379949452401	28.57055602358888	29.22240943555181	22.183235046335298
74-75	19.960193765796124	28.55960404380792	29.20977253580455	22.270429654591407
76	19.908593091828138	28.85804549283909	28.894271272114576	22.339090143218197
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	0.5
6	0.0
7	0.0
8	0.0
9	0.0
10	3.0
11	7.5
12	9.0
13	15.5
14	40.5
15	95.0
16	183.0
17	235.0
18	362.0
19	593.0
20	908.5
21	1120.0
22	1399.0
23	1978.0
24	2700.0
25	3122.0
26	3591.5
27	4623.5
28	5683.5
29	6181.0
30	6687.0
31	7773.5
32	8621.5
33	8889.0
34	9475.0
35	10461.0
36	11083.5
37	11306.0
38	11600.0
39	12022.5
40	12054.5
41	11713.0
42	11468.0
43	11147.5
44	10630.5
45	10015.5
46	9597.0
47	9400.0
48	8769.0
49	7848.0
50	7361.0
51	7063.0
52	6511.0
53	6003.5
54	5750.0
55	5569.5
56	5143.5
57	4647.5
58	4397.0
59	3908.5
60	3143.0
61	2570.0
62	2274.0
63	2033.5
64	1590.0
65	1282.0
66	1050.5
67	924.0
68	865.0
69	774.5
70	669.0
71	595.0
72	572.0
73	510.5
74	426.5
75	381.0
76	373.0
77	330.0
78	261.0
79	227.0
80	193.5
81	136.5
82	102.5
83	92.0
84	72.0
85	49.0
86	32.5
87	19.0
88	15.0
89	8.0
90	4.0
91	2.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	237400.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.79012853130159
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.34795446124727	69.00387862667733
2	13.736601504445686	22.745100082726584
3	2.345099801388952	5.824533419271635
4	0.41781739811318114	1.383646243696171
5	0.10080096625489038	0.4172662476160885
6	0.024017537359060582	0.11930490029771779
7	0.010325982911890755	0.05984226167412216
8	0.005132318575461547	0.03399242516208385
9	0.0020464105304001364	0.015248033175567274
>10	0.009184152819442607	0.1292122019798685
>50	5.09733176896678E-4	0.026164542643805692
>100	0.0	0.0
>500	5.09733176896678E-4	0.24181101507904293
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT	573	0.24136478517270432	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	4.2122999157540015E-4	0.0
6	0.0	0.0	0.0	4.2122999157540015E-4	0.0
7	0.0	0.0	0.0	4.2122999157540015E-4	0.0
8	0.0	0.0	0.0	4.2122999157540015E-4	0.0
9	0.0	0.0	0.0	8.424599831508003E-4	0.0
10	0.0	0.0	0.0	8.424599831508003E-4	0.0
11	0.0	0.0	0.0	8.424599831508003E-4	0.0
12	0.0	0.0	0.0	8.424599831508003E-4	0.0
13	0.0	0.0	0.0	0.0012636899747262005	0.0
14	0.0	0.0	0.0	0.0016849199663016006	0.0
15	0.0	0.0	0.0	0.0016849199663016006	0.0
16	0.0	0.0	0.0	0.0016849199663016006	0.0
17	0.0	0.0	0.0	0.0016849199663016006	0.0
18	0.0	0.0	0.0	0.0016849199663016006	0.0
19	0.0	0.0	0.0	0.0016849199663016006	0.0
20	0.0	0.0	0.0	0.0029486099410278013	0.0
21	0.0	0.0	0.0	0.0029486099410278013	0.0
22	0.0	0.0	0.0	0.0037910699241786015	0.0
23	0.0	0.0	0.0	0.0037910699241786015	0.0
24	0.0	0.0	0.0	0.004212299915754001	0.0
25	0.0	0.0	0.0	0.006318449873631003	0.0
26	0.0	0.0	0.0	0.006739679865206402	0.0
27	0.0	0.0	0.0	0.007582139848357203	0.0
28	0.0	0.0	0.0	0.011794439764111205	0.0
29	0.0	0.0	0.0	0.015585509688289806	0.0
30	0.0	0.0	0.0	0.019797809604043808	0.0
31	0.0	0.0	0.0	0.02443133951137321	0.0
32	0.0	0.0	0.0	0.031171019376579612	0.0
33	0.0	0.0	0.0	0.037910699241786014	0.0
34	0.0	0.0	0.0	0.04759898904802022	0.0
35	0.0	0.0	0.0	0.05602358887952822	0.0
36	0.0	0.0	0.0	0.07540016849199663	0.0
37	0.0	0.0	0.0	0.12047177759056445	0.0
38	0.0	0.0	0.0	0.19208087615838249	0.0
39	0.0	0.0	0.0	0.2860151642796967	0.0
40	0.0	0.0	0.0	0.4090143218197136	0.0
41	0.0	0.0	0.0	0.5341196293176074	0.0
42	0.0	0.0	0.0	0.6449031171019377	0.0
43	0.0	0.0	0.0	0.7586352148272957	0.0
44	0.0	0.0	0.0	0.8837405223251895	0.0
45	0.0	0.0	0.0	1.012636899747262	0.0
46	0.0	0.0	0.0	1.1672283066554339	0.0
47	0.0	0.0	0.0	1.343302443133951	0.0
48	0.0	0.0	0.0	1.5787700084246	0.0
49	0.0	0.0	0.0	1.8416175231676495	0.0
50	0.0	0.0	0.0	2.1044650379106993	0.0
51	0.0	0.0	0.0	2.355939342881213	0.0
52	0.0	0.0	0.0	2.5893007582139846	0.0
53	0.0	0.0	0.0	2.7973883740522325	0.0
54	0.0	0.0	0.0	3.0037910699241785	0.0
55	0.0	0.0	0.0	3.219882055602359	0.0
56	0.0	0.0	0.0	3.4271272114574556	0.0
57	0.0	0.0	0.0	3.650379106992418	0.0
58	0.0	0.0	0.0	3.8942712721145747	0.0
59	0.0	0.0	0.0	4.17312552653749	0.0
60	0.0	0.0	0.0	4.454085930918281	0.0
61	0.0	0.0	0.0	4.761583824768324	0.0
62	0.0	0.0	0.0	5.050126368997472	0.0
63	0.0	0.0	0.0	5.308340353833193	0.0
64	0.0	0.0	0.0	5.544229149115417	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGCCGT	100	0.0	52.499996	44
TATGCCG	95	0.0	51.57895	43
CGTATGC	105	0.0	50.000004	41
TCGTATG	105	0.0	50.000004	40
GCCGTCT	100	0.0	48.999996	46
CGTCTTC	95	0.0	47.89474	48
ATCTCGT	100	0.0	45.499996	37
CCGTCTT	100	0.0	45.499996	47
CTCGTAT	110	0.0	44.545452	39
TCTCGTA	105	0.0	43.333336	38
TGCCGTC	120	0.0	40.833336	45
AGCGTCA	35	0.0012537575	40.0	3
GCGTCAG	35	0.0012537575	40.0	4
TATCTCG	120	0.0	37.916668	36
GCGCGGC	40	0.0024155814	35.0	31
CCGCGCG	40	0.0024155814	35.0	29
GTATGCC	155	0.0	31.612904	42
CGCGCGG	45	0.0043016453	31.111109	30
CTGCTTG	180	0.0	29.166664	54
GGAGACG	50	0.0071990304	27.999998	6
>>END_MODULE