FastQCFastQC Report
Wed 25 May 2016
SRR1781134_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781134_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120947
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTATC150.002220078870.000017
CAGAAAC200.00693707552.514
ATGACAC200.00693707552.570
GTGGTAC200.00693707552.514
GCCAAAA200.00693707552.519
TTGTTCG305.8584375E-446.6666689
GTCGGTT350.001251317740.060
TCACATA400.002410909435.09
TTGAGCT400.002410909435.04
TGTAGTT400.002410909435.023
TGAGGGC400.002410909435.054
GGTTCAG450.00429337531.11111312
GATTATC450.00429337531.11111366
AGTCATT500.00718527528.00000238
TATGGTT500.00718527528.0000024
CCGTGCG500.00718527528.0000029
GGTAGTA750.001823604323.3333341
AAAGAAC850.003771467120.58823640
GATCCGT1408.151448E-620.070
TATATAG900.00525013819.4444455
Produced by FastQC (version 0.11.5)