##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781134_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	120947
Sequences flagged as poor quality	0
Sequence length	76
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.755157217624248	34.0	32.0	34.0	21.0	34.0
2	30.929340950995066	34.0	32.0	34.0	21.0	34.0
3	30.965364994584405	34.0	32.0	34.0	21.0	34.0
4	31.13221493712122	34.0	32.0	34.0	23.0	34.0
5	31.15634120730568	34.0	32.0	34.0	23.0	34.0
6	33.79328962272731	37.0	34.0	38.0	23.0	38.0
7	33.710898162004845	37.0	34.0	38.0	22.0	38.0
8	33.44744392171778	37.0	34.0	38.0	21.0	38.0
9	33.80032576252408	37.0	34.0	38.0	23.0	38.0
10-11	33.67950424566132	37.0	34.0	38.0	22.0	38.0
12-13	33.625381365391455	37.0	34.0	38.0	22.0	38.0
14-15	33.49058678594757	37.0	34.0	38.0	21.5	38.0
16-17	33.45390129560882	37.0	34.0	38.0	21.5	38.0
18-19	33.46906496233888	37.0	34.0	38.0	21.0	38.0
20-21	33.41873713279371	37.0	34.0	38.0	21.5	38.0
22-23	33.47904867421267	37.0	34.0	38.0	21.5	38.0
24-25	33.462653062911855	37.0	34.0	38.0	21.0	38.0
26-27	33.375333823906345	37.0	34.0	38.0	21.0	38.0
28-29	33.46286803310541	37.0	34.0	38.0	21.0	38.0
30-31	33.53495332666375	37.0	34.0	38.0	22.0	38.0
32-33	33.488759539302336	37.0	34.0	38.0	21.5	38.0
34-35	33.50867735454372	37.0	34.0	38.0	22.0	38.0
36-37	33.322339537152644	37.0	34.0	38.0	21.0	38.0
38-39	33.36494910994072	37.0	34.0	38.0	21.0	38.0
40-41	33.37318412197078	37.0	34.0	38.0	21.0	38.0
42-43	33.421916211233025	37.0	34.0	38.0	21.0	38.0
44-45	33.44378942842732	37.0	34.0	38.0	21.5	38.0
46-47	33.469259262321515	37.0	34.0	38.0	21.5	38.0
48-49	33.407339578493065	37.0	34.0	38.0	21.0	38.0
50-51	33.27924214738687	37.0	34.0	38.0	21.0	38.0
52-53	33.365970218360104	37.0	34.0	38.0	21.0	38.0
54-55	33.314898261221856	37.0	34.0	38.0	21.0	38.0
56-57	33.268766484493206	37.0	34.0	38.0	21.0	38.0
58-59	33.267625488850484	37.0	34.0	38.0	21.0	38.0
60-61	33.2709327225975	37.0	34.0	38.0	21.0	38.0
62-63	33.20873192390055	37.0	34.0	38.0	21.0	38.0
64-65	33.20676825386326	37.0	34.0	38.0	21.0	38.0
66-67	33.12007738926968	37.0	33.0	38.0	21.0	38.0
68-69	32.85757397868488	37.0	32.0	38.0	20.0	38.0
70-71	32.98863965207901	37.0	33.0	38.0	21.0	38.0
72-73	32.94175547967292	37.0	32.0	38.0	21.0	38.0
74-75	32.916955360612505	37.0	32.0	38.0	21.0	38.0
76	31.474001008706292	36.0	27.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	12.0
14	84.0
15	246.0
16	648.0
17	1084.0
18	1464.0
19	1553.0
20	1407.0
21	1321.0
22	1307.0
23	1465.0
24	1674.0
25	1918.0
26	2218.0
27	2652.0
28	3069.0
29	3563.0
30	4217.0
31	5021.0
32	6142.0
33	7319.0
34	9079.0
35	11685.0
36	17229.0
37	34568.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	46.720464335618075	17.148006978263204	9.331360017197616	26.8001686689211
2	24.016304662372775	19.64827569100515	30.694436406029084	25.640983240592984
3	20.988532166982232	20.741316444393	28.562924256079107	29.70722713254566
4	15.13059439258518	15.806096885412618	35.074867503948006	33.98844121805419
5	15.261230125592201	32.28769626365268	34.7085913664663	17.74248224428882
6	30.72833555193597	34.622603289043965	18.537871960445486	16.111189198574582
7	29.738645853142287	27.5170115835862	21.894714213663836	20.84962834960768
8	26.279279353766526	32.62586091428477	19.983959916326985	21.11089981562172
9	24.977056065880095	15.589473074983257	20.987705358545476	38.445765500591165
10-11	26.557500392734006	22.962123905512332	26.547992095711344	23.932383606042315
12-13	26.245380207859643	21.534639139457777	25.323488800879723	26.896491851802857
14-15	24.825750121954243	22.63842840252342	22.903833910721225	29.63198756480111
16-17	25.141177540575626	25.06511116439432	24.053511042026674	25.74020025300338
18-19	24.52189802144741	24.692633963636965	25.051468825187897	25.73399918972773
20-21	24.51817738348202	24.951011600122367	25.147378603851273	25.383432412544337
22-23	25.12794860558757	24.393329309532273	25.113066053726012	25.365656031154142
24-25	24.665762689442484	24.660388434603586	24.808387144782426	25.865461731171507
26-27	24.067980189669854	24.832777993666646	25.442135811553822	25.657106005109675
28-29	24.521071213010657	24.691393750981835	25.953103425467354	24.834431610540154
30-31	24.73397438547463	24.35818995097026	25.339611565396414	25.568224098158698
32-33	24.355296121441622	25.09818350186445	25.041133719728474	25.505386656965445
34-35	25.21931093784881	23.93321041447907	25.502079423218433	25.345399224453686
36-37	25.41278411204908	23.705011285935164	24.83236458944827	26.049840012567486
38-39	24.237889323422657	24.239129536077787	26.101515539864568	25.421465600634992
40-41	24.408625265612212	23.52270002563106	26.659197830454662	25.40947687830207
42-43	24.179185924413176	23.937344456662835	26.032890439614047	25.850579179309946
44-45	23.52890108890671	24.20771081548116	26.510785716057445	25.75260237955468
46-47	23.83812744425244	23.942718711501733	26.622404855019138	25.596748989226686
48-49	23.30979685316709	24.051030616716414	27.14825502079423	25.490917509322266
50-51	23.17709409906819	24.320570167097983	26.38717785476283	26.115157879071
52-53	22.962537309730706	24.044829553440763	27.05110502947572	25.941528107352806
54-55	22.655377975476863	24.490479300850787	26.786939733933046	26.067202989739307
56-57	22.403614806485486	24.727359917980603	26.854324621528434	26.014700654005473
58-59	23.084078149933443	24.227140813744864	26.293335097191335	26.39544593913036
60-61	22.639255210960172	23.771155960875426	26.89690525602123	26.69268357214317
62-63	22.134075256103912	23.721960858888604	27.183807783574625	26.960156101432858
64-65	22.97948688268415	24.067566785451476	26.43513274409452	26.51781358776985
66-67	22.760796051162906	23.93197020182394	25.939461086260923	27.36777266075223
68-69	22.454463525345815	24.23871613185941	26.080018520508986	27.226801822285797
70-71	22.992715817672206	23.79761383085153	26.11391766641587	27.095752685060397
72-73	23.242825369790072	23.239931540261438	26.001058314799042	27.516184775149444
74-75	22.996436455637593	23.504510240022487	26.083739158474373	27.415314145865544
76	23.19528388467676	22.92078348367467	25.495464955724405	28.38846767592417
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	2.5
15	2.5
16	5.5
17	11.0
18	16.5
19	24.5
20	30.5
21	34.0
22	54.0
23	108.0
24	168.5
25	195.0
26	262.5
27	393.5
28	525.0
29	593.0
30	691.0
31	887.0
32	1316.5
33	1648.0
34	1843.0
35	2320.0
36	2928.0
37	3254.0
38	3671.0
39	4668.5
40	5610.0
41	6247.0
42	6523.0
43	6689.0
44	6968.5
45	7100.5
46	7119.0
47	7039.0
48	6566.5
49	5770.5
50	5367.0
51	5005.0
52	4165.5
53	3508.0
54	3328.0
55	3103.5
56	2643.0
57	2218.0
58	2029.0
59	1870.0
60	1677.5
61	1722.0
62	1800.0
63	1782.5
64	1801.5
65	1844.5
66	1844.0
67	1837.0
68	1862.5
69	1878.0
70	1782.5
71	1697.0
72	1728.0
73	1680.0
74	1510.0
75	1419.0
76	1319.5
77	1106.0
78	902.0
79	812.0
80	706.5
81	510.0
82	338.5
83	258.0
84	220.0
85	141.0
86	75.5
87	51.0
88	39.5
89	20.5
90	11.0
91	8.5
92	8.0
93	6.0
94	3.0
95	1.5
96	1.0
97	0.5
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	120947.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.341595905644624
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.65763289210267	40.76330954881063
2	12.428661069112858	13.756438770701216
3	5.269369826993755	8.748460069286548
4	2.5487793946275437	5.642140772404441
5	1.4626348343143993	4.047227297907348
6	1.00845609107479	3.348574168850819
7	0.7245943765500344	2.807014642777415
8	0.5542773478351809	2.453967440283761
9	0.39591239131084355	1.9719381216565932
>10	1.9466937580302985	16.369980239278362
>50	0.0029880180476290078	0.09094892804286175
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0033072337470131546	0.0
2	0.0	0.0	0.0	0.0033072337470131546	0.0
3	0.0	0.0	0.0	0.0033072337470131546	0.0
4	0.0	0.0	0.0	0.004960850620519732	0.0
5	0.0	0.0	0.0	0.00578765905727302	0.0
6	0.0	0.0	0.0	0.006614467494026309	0.0
7	0.0	0.0	0.0	0.006614467494026309	0.0
8	0.0	0.0	0.0	0.007441275930779597	0.0
9	0.0	0.0	0.0	0.007441275930779597	0.0
10	0.0	0.0	0.0	0.007441275930779597	0.0
11	0.0	0.0	0.0	0.007441275930779597	0.0
12	0.0	0.0	0.0	0.007441275930779597	0.0
13	0.0	0.0	0.0	0.008268084367532886	0.0
14	0.0	0.0	0.0	0.009094892804286174	0.0016536168735065773
15	0.0	0.0	0.0	0.009094892804286174	0.0016536168735065773
16	0.0	0.0	0.0	0.009094892804286174	0.0016536168735065773
17	0.0	0.0	0.0	0.009094892804286174	0.0016536168735065773
18	0.0	0.0	0.0	0.009094892804286174	0.0016536168735065773
19	0.0	0.0	0.0	0.01157531811454604	0.0016536168735065773
20	0.0	0.0	0.0	0.01157531811454604	0.0016536168735065773
21	0.0	0.0	0.0	0.014055743424805906	0.0016536168735065773
22	0.0	0.0	0.0	0.015709360298312484	0.0016536168735065773
23	0.0	0.0	0.0	0.01736297717181906	0.0016536168735065773
24	0.0	0.0	0.0	0.020670210918832217	0.0016536168735065773
25	0.0	0.0	0.0	0.021497019355585505	0.0016536168735065773
26	0.0	0.0	0.0	0.02397744466584537	0.0016536168735065773
27	0.0	0.0	0.0	0.038859996527404565	0.0016536168735065773
28	0.0	0.0	0.0	0.052915739952210473	0.0016536168735065773
29	0.0	0.0	0.0	0.07193233399753611	0.0016536168735065773
30	0.0	0.0	0.0	0.09260254491636832	0.0016536168735065773
31	0.0	0.0	0.0	0.1091387136514341	0.0016536168735065773
32	0.0	0.0	0.0	0.1471719017420854	0.0016536168735065773
33	0.0	0.0	0.0	0.20091445013104914	0.0016536168735065773
34	0.0	0.0	0.0	0.2902097613004043	0.0016536168735065773
35	0.0	0.0	0.0	0.4051361340091114	0.0016536168735065773
36	0.0	0.0	0.0	0.5828999479110685	0.0016536168735065773
37	0.0	0.0	0.0	0.8499590729823807	0.0016536168735065773
38	0.0	0.0	0.0	1.1153645811801864	0.0016536168735065773
39	0.0	0.0	0.0	1.429551787146436	0.0016536168735065773
40	0.0	0.0	0.0	1.773504096835804	0.0016536168735065773
41	0.0	0.0	0.0	2.157970019926083	0.0016536168735065773
42	0.0	0.0	0.0	2.4804253102598657	0.0016536168735065773
43	0.0	0.0	0.0	2.927728674543395	0.0016536168735065773
44	0.0	0.0	0.0	3.4105848016073157	0.0016536168735065773
45	0.0	0.0	0.0	3.9364349673824073	0.0016536168735065773
46	0.0	0.0	0.0	4.488743003133604	0.0016536168735065773
47	0.0	0.0	0.0	5.097274012584024	0.0016536168735065773
48	0.0	0.0	0.0	5.685134811115613	0.0016536168735065773
49	0.0	0.0	0.0	6.201063275649664	0.0016536168735065773
50	0.0	0.0	0.0	6.741795993286315	0.0016536168735065773
51	0.0	0.0	0.0	7.225478928786989	0.0016536168735065773
52	0.0	0.0	0.0	7.7190835655287025	0.0016536168735065773
53	0.0	0.0	0.0	8.186230332294311	0.0016536168735065773
54	0.0	0.0	0.0	8.674047309978752	0.0016536168735065773
55	0.0	0.0	0.0	9.23214300478722	0.0016536168735065773
56	0.0	0.0	0.0	9.827445079249589	0.0016536168735065773
57	0.0	0.0	0.0	10.35081481971442	0.0016536168735065773
58	0.0	0.0	0.0	10.958519020728087	0.0016536168735065773
59	0.0	0.0	0.0	11.514134290226297	0.0016536168735065773
60	0.0	0.0	0.0	12.002778076347491	0.0016536168735065773
61	0.0	0.0	0.0	12.50051675527297	0.0016536168735065773
62	0.0	0.0	0.0	13.011484369186503	0.0016536168735065773
63	0.0	0.0	0.0	13.518317940916269	0.0016536168735065773
64	0.0	0.0	0.0	14.030939171703308	0.0016536168735065773
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTTATC	15	0.0022200788	70.00001	7
CAGAAAC	20	0.006937075	52.5	14
ATGACAC	20	0.006937075	52.5	70
GTGGTAC	20	0.006937075	52.5	14
GCCAAAA	20	0.006937075	52.5	19
TTGTTCG	30	5.8584375E-4	46.666668	9
GTCGGTT	35	0.0012513177	40.0	60
TCACATA	40	0.0024109094	35.0	9
TTGAGCT	40	0.0024109094	35.0	4
TGTAGTT	40	0.0024109094	35.0	23
TGAGGGC	40	0.0024109094	35.0	54
GGTTCAG	45	0.004293375	31.111113	12
GATTATC	45	0.004293375	31.111113	66
AGTCATT	50	0.007185275	28.000002	38
TATGGTT	50	0.007185275	28.000002	4
CCGTGCG	50	0.007185275	28.000002	9
GGTAGTA	75	0.0018236043	23.333334	1
AAAGAAC	85	0.0037714671	20.588236	40
GATCCGT	140	8.151448E-6	20.0	70
TATATAG	90	0.005250138	19.444445	5
>>END_MODULE