FastQCFastQC Report
Wed 25 May 2016
SRR1781134_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781134_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110352
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT38053.4480571262867916No Hit
TCTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG3740.3389154704944179No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATACAGACCTCGTATGCCGT2650.24014064085834422No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCC2410.21839205451645644No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATACAGAACTCGTATGCCGT1920.17398869073510223No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATTG150.002219563870.045
ACGTTAC150.002219563870.017
TTACTCG150.002219563870.020
ATATGTC150.002219563870.011
GATCTTA150.002219563870.01
TCGACAT150.002219563870.069
TATGCCG6800.056.1029443
CGTATGC6750.056.041
ACAGATC5750.056.033
CCATACA252.3815445E-455.9999962
TCGTATG6700.055.89552340
TCTCGTA5950.055.8823538
AGATCTC5800.055.51724635
ATGCCGT6750.055.4814844
CCGTCTT6650.055.26315747
ATCTCGT5900.055.16949537
TGCCGTC6750.054.96296345
GCCGTCT6700.054.85074646
GTATGCC6900.054.7826142
CTCGTAT6650.054.73684339
Produced by FastQC (version 0.11.5)