##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781134_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	110352
Sequences flagged as poor quality	0
Sequence length	76
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.26883065100768	34.0	34.0	34.0	32.0	34.0
2	33.259415325503845	34.0	34.0	34.0	32.0	34.0
3	33.29380527765695	34.0	34.0	34.0	33.0	34.0
4	33.35926852254603	34.0	34.0	34.0	33.0	34.0
5	33.35631433956793	34.0	34.0	34.0	33.0	34.0
6	36.76525119617225	38.0	37.0	38.0	34.0	38.0
7	36.65224916630419	38.0	37.0	38.0	34.0	38.0
8	36.46759460635059	38.0	37.0	38.0	34.0	38.0
9	36.423707771494854	38.0	37.0	38.0	34.0	38.0
10-11	36.31661410758301	38.0	37.0	38.0	34.0	38.0
12-13	36.260625090619115	38.0	37.0	38.0	34.0	38.0
14-15	36.25892145135566	38.0	37.0	38.0	34.0	38.0
16-17	36.16639480933739	38.0	37.0	38.0	33.0	38.0
18-19	36.142276533275336	38.0	37.0	38.0	33.0	38.0
20-21	36.090419747716396	38.0	36.5	38.0	32.5	38.0
22-23	36.15803066550674	38.0	37.0	38.0	33.0	38.0
24-25	36.14986588371756	38.0	37.0	38.0	33.0	38.0
26-27	35.933023415977964	38.0	36.0	38.0	31.5	38.0
28-29	35.91148778454401	38.0	36.0	38.0	31.0	38.0
30-31	35.94964749166304	38.0	37.0	38.0	32.0	38.0
32-33	35.907011200521964	38.0	36.0	38.0	31.5	38.0
34-35	35.88380364651297	38.0	36.0	38.0	31.5	38.0
36-37	35.78486117152385	38.0	36.0	38.0	31.0	38.0
38-39	35.441287878787875	38.0	36.0	38.0	31.0	38.0
40-41	35.706643286936355	38.0	36.0	38.0	31.0	38.0
42-43	35.81070574162679	38.0	36.0	38.0	31.0	38.0
44-45	35.7784045599536	38.0	36.0	38.0	31.0	38.0
46-47	35.806396802957806	38.0	36.0	38.0	31.0	38.0
48-49	35.76368348557344	38.0	36.0	38.0	31.0	38.0
50-51	35.649702769319994	38.0	36.0	38.0	31.0	38.0
52-53	35.642095295055825	38.0	36.0	38.0	31.0	38.0
54-55	35.68758608815427	38.0	36.0	38.0	31.0	38.0
56-57	35.63335055096418	38.0	36.0	38.0	31.0	38.0
58-59	35.5683766492678	38.0	36.0	38.0	31.0	38.0
60-61	35.565458713933594	38.0	36.0	38.0	31.0	38.0
62-63	35.549695519791214	38.0	36.0	38.0	31.0	38.0
64-65	35.4992116137451	38.0	36.0	38.0	31.0	38.0
66-67	35.43926254168479	38.0	36.0	38.0	31.0	38.0
68-69	35.28144483108598	38.0	36.0	38.0	29.5	38.0
70-71	34.96581847179933	38.0	35.5	38.0	27.0	38.0
72-73	34.694867333623314	38.0	35.5	38.0	25.0	38.0
74-75	34.72300003624764	38.0	36.0	38.0	25.0	38.0
76	33.80624728142671	37.0	34.0	38.0	23.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	2.0
21	5.0
22	15.0
23	40.0
24	115.0
25	242.0
26	414.0
27	724.0
28	1233.0
29	1825.0
30	2765.0
31	3837.0
32	5341.0
33	7166.0
34	9428.0
35	12992.0
36	18365.0
37	45843.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.87005219660722	16.79897056691315	9.03653762505437	29.294439611425254
2	23.773923444976077	19.245686530375526	31.988545744526604	24.991844280121793
3	20.538821226620268	20.07847614905031	30.81774684645498	28.56495577787444
4	14.787226330288531	19.455016673916194	33.74474409163405	32.01301290416123
5	14.67214006089604	31.9377990430622	36.57840365376251	16.811657242279253
6	30.13719733217341	38.35000724952878	16.969334493257936	14.543460925039872
7	28.96186747861389	27.50289981151225	20.15912715673481	23.376105553139045
8	25.185769175003625	36.13799478033928	17.69881832680876	20.97741771784834
9	24.084746991445556	15.330034797738145	18.71647092938959	41.86874728142671
10-11	26.10102218355807	24.684645498042627	26.35520878642888	22.859123531970422
12-13	25.31807307525011	20.722778019428738	25.847741771784833	28.11140713353632
14-15	23.813342757720747	21.37659489633174	21.429607075540087	33.380455270407424
16-17	26.610301580397273	26.009043787153836	22.4354791938524	24.945175438596493
18-19	26.47256053356532	24.14863346382485	22.924822386544875	26.453983616064956
20-21	23.937037842540235	23.83282586631869	23.066641293315936	29.16349499782514
22-23	24.232003044802088	25.775246483978542	23.35345077569958	26.639299695519792
24-25	26.270026823256487	25.920690155139916	23.11149775264608	24.697785268957517
26-27	25.70637596056256	26.40323691460055	23.42096201246919	24.469425112367695
28-29	23.97464477308975	25.839132956357837	24.241064955777876	25.94515731477454
30-31	23.883119472234306	28.31892489488183	23.408728432651877	24.389227200231986
32-33	23.373386979846313	26.883971291866025	25.716344062635926	24.026297665651732
34-35	24.29271784833986	25.817837465564736	23.667899086559373	26.22154559953603
36-37	26.76344787588807	25.472578657387267	23.099264172828764	24.664709293895896
38-39	23.76486153400029	24.159054661447005	25.993638538494995	26.082445266057707
40-41	23.418243439176454	22.91440118892272	27.00358851674641	26.663766855154414
42-43	25.72268740031898	23.138230390024646	26.399159054661446	24.739923154994926
44-45	22.55192474989126	26.00541902276352	26.962356821806583	24.48029940553864
46-47	25.16039582427142	23.17719660722053	24.967830216035956	26.694577352472088
48-49	24.976892127011745	23.046251993620416	24.937019718718282	27.039836160649557
50-51	22.629857184283022	23.186711613745107	27.012650427722196	27.170780774249675
52-53	21.94885457445266	23.006832680875743	29.954146730462522	25.090166014209075
54-55	21.446371610845294	23.37248078874873	27.72310424822387	27.45804335218211
56-57	23.62349572277802	23.297266927649705	25.45807959982601	27.621157749746267
58-59	21.916231694939828	23.259659997100187	29.135403073800205	25.68870523415978
60-61	24.001830506017107	25.26687327823691	25.17580107293026	25.555495142815715
62-63	20.71643468174569	28.17030955487893	25.14227200231985	25.970983761055532
64-65	21.27600768450051	28.606187472814266	24.133681310714802	25.984123531970422
66-67	21.373423227490214	28.38870160939539	23.772564158329708	26.465311004784688
68-69	20.961559373640714	29.016238944468608	23.833732057416267	26.188469624474408
70-71	21.535178338408002	27.914763665361754	23.836903726257795	26.71315426997245
72-73	21.398796578222417	26.259605625634336	24.313107148035378	28.028490648107873
74-75	21.259243149195303	26.378769754965926	24.666068580542262	27.69591851529651
76	21.74043062200957	26.088335508191967	24.06934174278672	28.101892127011745
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.0
14	1.5
15	3.0
16	11.0
17	17.0
18	15.5
19	18.0
20	25.0
21	28.0
22	31.0
23	61.5
24	124.0
25	159.0
26	237.0
27	316.5
28	412.5
29	507.0
30	584.0
31	753.0
32	1131.5
33	1418.0
34	1530.5
35	1913.5
36	2428.5
37	2673.0
38	3362.0
39	4873.0
40	6259.0
41	6880.5
42	6938.0
43	6907.5
44	6592.0
45	6234.0
46	6161.0
47	6128.5
48	5699.5
49	4976.0
50	4649.0
51	4254.0
52	3471.0
53	2946.0
54	2809.0
55	2585.5
56	2193.5
57	1925.0
58	1825.0
59	1639.0
60	1435.0
61	1440.5
62	1464.0
63	1518.0
64	1525.0
65	1524.5
66	1577.0
67	1583.0
68	1637.0
69	1734.5
70	1722.0
71	1666.0
72	1673.0
73	1623.0
74	1422.0
75	1278.0
76	1195.5
77	1035.5
78	843.5
79	729.0
80	630.5
81	476.5
82	337.5
83	254.0
84	212.5
85	119.5
86	62.0
87	56.0
88	42.0
89	21.0
90	11.5
91	6.0
92	3.0
93	2.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	110352.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.24615774974627
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.21834335645286	26.042119762215453
2	18.951029901724535	16.391184573002754
3	8.05062548456719	10.444758590691606
4	3.801102193910693	6.575322604030738
5	2.2442009094147477	4.8526533275337105
6	1.4542254258952705	3.77337973031753
7	1.0665716740355804	3.2287588806727565
8	0.7815937807765648	2.704074235174714
9	0.5762420635752153	2.242822966507177
>10	2.830920101418603	18.896802957807743
>50	0.012572554114368334	0.33710308829926056
>100	0.010477128428640278	1.0629621574597652
>500	0.0	0.0
>1k	0.0020954256857280557	3.4480571262867916
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT	3805	3.4480571262867916	No Hit
TCTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG	374	0.3389154704944179	No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATACAGACCTCGTATGCCGT	265	0.24014064085834422	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCC	241	0.21839205451645644	No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATACAGAACTCGTATGCCGT	192	0.17398869073510223	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.004530955487893287	0.0
2	0.0	0.0	0.0	0.004530955487893287	0.0
3	0.0	0.0	0.0	0.004530955487893287	0.0
4	0.0	0.0	0.0	0.006343337683050601	0.0
5	0.0	0.0	0.0	0.007249528780629259	0.0
6	0.0	0.0	0.0	0.008155719878207917	0.0
7	0.0	0.0	0.0	0.008155719878207917	0.0
8	0.0	0.0	0.0	0.009061910975786574	0.0
9	0.0	0.0	0.0	0.009061910975786574	0.0
10	0.0	0.0	0.0	0.009061910975786574	0.0
11	0.0	0.0	0.0	0.009061910975786574	0.0
12	0.0	0.0	0.0	0.009061910975786574	0.0
13	0.0	0.0	0.0	0.009968102073365232	0.0
14	0.0	0.0	0.0	0.009968102073365232	0.0018123821951573148
15	0.0	0.0	0.0	0.009968102073365232	0.0018123821951573148
16	0.0	0.0	0.0	0.009968102073365232	0.0018123821951573148
17	0.0	0.0	0.0	0.009968102073365232	0.0018123821951573148
18	0.0	0.0	0.0	0.01087429317094389	0.0018123821951573148
19	0.0	0.0	0.0	0.016311439756415833	0.0018123821951573148
20	0.0	0.0	0.0	0.016311439756415833	0.0018123821951573148
21	0.0	0.0	0.0	0.01721763085399449	0.0018123821951573148
22	0.0	0.0	0.0	0.01812382195157315	0.0018123821951573148
23	0.0	0.0	0.0	0.019936204146730464	0.0018123821951573148
24	0.0	0.0	0.0	0.02174858634188778	0.0018123821951573148
25	0.0	0.0	0.0	0.02356096853704509	0.0018123821951573148
26	0.0	0.0	0.0	0.028998115122517036	0.0018123821951573148
27	0.0	0.0	0.0	0.04440336378135421	0.0018123821951573148
28	0.0	0.0	0.0	0.0616209946353487	0.0018123821951573148
29	0.0	0.0	0.0	0.08065100768450051	0.0018123821951573148
30	0.0	0.0	0.0	0.10149340292880962	0.0018123821951573148
31	0.0	0.0	0.0	0.12052341597796143	0.0018123821951573148
32	0.0	0.0	0.0	0.16039582427142235	0.0018123821951573148
33	0.0	0.0	0.0	0.2211106278091924	0.0018123821951573148
34	0.0	0.0	0.0	0.31807307525010875	0.0018123821951573148
35	0.0	0.0	0.0	0.44493982891112077	0.0018123821951573148
36	0.0	0.0	0.0	0.6388647237929534	0.0018123821951573148
37	0.0	0.0	0.0	0.9360954037987531	0.0018123821951573148
38	0.0	0.0	0.0	1.2260765550239234	0.0018123821951573148
39	0.0	0.0	0.0	1.5622734522256054	0.0018123821951573148
40	0.0	0.0	0.0	1.9401551399159054	0.0018123821951573148
41	0.0	0.0	0.0	2.3415977961432506	0.0018123821951573148
42	0.0	0.0	0.0	2.685044222125562	0.0018123821951573148
43	0.0	0.0	0.0	3.1807307525010873	0.0018123821951573148
44	0.0	0.0	0.0	3.700884442511237	0.0018123821951573148
45	0.0	0.0	0.0	4.256379585326954	0.0018123821951573148
46	0.0	0.0	0.0	4.844497607655502	0.0018123821951573148
47	0.0	0.0	0.0	5.488799478033928	0.0018123821951573148
48	0.0	0.0	0.0	6.124945628534145	0.0018123821951573148
49	0.0	0.0	0.0	6.66866028708134	0.0018123821951573148
50	0.0	0.0	0.0	7.235029723068001	0.0018123821951573148
51	0.0	0.0	0.0	7.7452153110047846	0.0018123821951573148
52	0.0	0.0	0.0	8.270806147600405	0.0018123821951573148
53	0.0	0.0	0.0	8.786428882122662	0.0018123821951573148
54	0.0	0.0	0.0	9.309301145425547	0.0018123821951573148
55	0.0	0.0	0.0	9.911012034217777	0.0018123821951573148
56	0.0	0.0	0.0	10.54897056691315	0.0018123821951573148
57	0.0	0.0	0.0	11.13618239814412	0.0018123821951573148
58	0.0	0.0	0.0	11.761454255473394	0.0018123821951573148
59	0.0	0.0	0.0	12.374945628534146	0.0018123821951573148
60	0.0	0.0	0.0	12.89872408293461	0.0018123821951573148
61	0.0	0.0	0.0	13.41072205306655	0.0018123821951573148
62	0.0	0.0	0.0	13.960780049296796	0.0018123821951573148
63	0.0	0.0	0.0	14.480027548209366	0.0018123821951573148
64	0.0	0.0	0.0	15.021929824561404	0.0018123821951573148
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATTG	15	0.0022195638	70.0	45
ACGTTAC	15	0.0022195638	70.0	17
TTACTCG	15	0.0022195638	70.0	20
ATATGTC	15	0.0022195638	70.0	11
GATCTTA	15	0.0022195638	70.0	1
TCGACAT	15	0.0022195638	70.0	69
TATGCCG	680	0.0	56.10294	43
CGTATGC	675	0.0	56.0	41
ACAGATC	575	0.0	56.0	33
CCATACA	25	2.3815445E-4	55.999996	2
TCGTATG	670	0.0	55.895523	40
TCTCGTA	595	0.0	55.88235	38
AGATCTC	580	0.0	55.517246	35
ATGCCGT	675	0.0	55.48148	44
CCGTCTT	665	0.0	55.263157	47
ATCTCGT	590	0.0	55.169495	37
TGCCGTC	675	0.0	54.962963	45
GCCGTCT	670	0.0	54.850746	46
GTATGCC	690	0.0	54.78261	42
CTCGTAT	665	0.0	54.736843	39
>>END_MODULE