FastQCFastQC Report
Wed 25 May 2016
SRR1781133_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781133_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences242989
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCTT352.0825717E-550.045
TCGTTGT604.9130904E-946.66666834
CATCGTT604.9130904E-946.66666832
GAACCGT305.870202E-446.66666869
TATACCG305.870202E-446.6666686
CAATACG451.6802678E-646.66666469
AACTACA701.9175786E-840.046
CCTCGTA400.002415692435.022
CTAGGGA501.7065236E-435.04
GTACCAT652.09724E-532.3076936
GATCGTT552.9865606E-431.8181844
GACTGCA552.9865606E-431.818187
AGGGATC552.9865606E-431.8181841
GTAGCAA450.004301841431.1111098
TACCTAG450.004301841431.11110933
AGTTAGA450.004301841431.11110936
ATACCGC450.004301841431.1111097
GCACTCG450.004301841431.1111099
GTATTTC1052.0127118E-829.99999839
GTCTATG604.9728673E-429.1666682