FastQCFastQC Report
Wed 25 May 2016
SRR1781133_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781133_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences219768
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGT36331.6531069127443485No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG3940.17927996796621892No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCC2760.12558698263623458No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCGT150.002222504270.037
CGCAATA150.002222504270.028
CACCGAA150.002222504270.09
CGTTGAA200.00694459552.516
CCGTCTT5200.052.547
AAAAACG200.00694459552.567
CTCGAAA200.00694459552.557
CCGTTTG200.00694459552.563
CGATAGA200.00694459552.58
TCGTATG5500.052.18181640
TATGCCG5450.052.0183543
CGTCTTC5350.051.68224348
CTCGTAT5250.051.33333239
ATGCCGT5550.051.0810844
CGTATGC5700.050.9649141
GCCGTCT5600.050.046
TCTCGTA4900.049.99999638
CTTGAAA5500.048.99999657
CTGCTTG5650.047.69911654
GTATGCC6100.047.0491842
Produced by FastQC (version 0.11.5)