##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781133_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	219768
Sequences flagged as poor quality	0
Sequence length	76
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.3338202104037	34.0	34.0	34.0	33.0	34.0
2	33.31343962724328	34.0	34.0	34.0	33.0	34.0
3	33.355265552764735	34.0	34.0	34.0	33.0	34.0
4	33.41707163918314	34.0	34.0	34.0	34.0	34.0
5	33.411247315350735	34.0	34.0	34.0	34.0	34.0
6	36.85819136543992	38.0	37.0	38.0	34.0	38.0
7	36.76544810891485	38.0	37.0	38.0	34.0	38.0
8	36.568945433366096	38.0	37.0	38.0	34.0	38.0
9	36.50972389064832	38.0	37.0	38.0	34.0	38.0
10-11	36.417005660514725	38.0	37.0	38.0	34.0	38.0
12-13	36.3569059189691	38.0	37.0	38.0	34.0	38.0
14-15	36.36236167230898	38.0	37.0	38.0	34.0	38.0
16-17	36.32489716428233	38.0	37.0	38.0	34.0	38.0
18-19	36.31643141858687	38.0	37.0	38.0	34.0	38.0
20-21	36.24703778530086	38.0	37.0	38.0	34.0	38.0
22-23	36.30503530996323	38.0	37.0	38.0	34.0	38.0
24-25	36.30832286775145	38.0	37.0	38.0	34.0	38.0
26-27	36.0942493902661	38.0	37.0	38.0	33.0	38.0
28-29	36.076007426012886	38.0	37.0	38.0	32.5	38.0
30-31	36.12219476902916	38.0	37.0	38.0	33.5	38.0
32-33	36.06488205744239	38.0	37.0	38.0	32.0	38.0
34-35	36.052371136835205	38.0	37.0	38.0	32.5	38.0
36-37	36.02846410760438	38.0	37.0	38.0	32.0	38.0
38-39	35.89499836190892	38.0	37.0	38.0	32.0	38.0
40-41	35.99287657893779	38.0	37.0	38.0	32.0	38.0
42-43	36.01355065341633	38.0	37.0	38.0	32.0	38.0
44-45	35.98138263987478	38.0	37.0	38.0	32.0	38.0
46-47	35.99810026937497	38.0	37.0	38.0	32.0	38.0
48-49	35.980820683630014	38.0	37.0	38.0	32.0	38.0
50-51	35.865501346874886	38.0	36.5	38.0	31.5	38.0
52-53	35.89905491245313	38.0	37.0	38.0	32.0	38.0
54-55	35.91209366240763	38.0	37.0	38.0	32.0	38.0
56-57	35.86951011976266	38.0	37.0	38.0	32.0	38.0
58-59	35.84856530523097	38.0	36.5	38.0	31.5	38.0
60-61	35.84904080666885	38.0	36.0	38.0	31.5	38.0
62-63	35.83636607695388	38.0	36.0	38.0	31.0	38.0
64-65	35.79288840959558	38.0	36.0	38.0	31.0	38.0
66-67	35.764961231844495	38.0	36.0	38.0	31.0	38.0
68-69	35.664955771540896	38.0	36.0	38.0	31.0	38.0
70-71	35.517978049579554	38.0	36.0	38.0	31.0	38.0
72-73	35.41459175130137	38.0	36.0	38.0	31.0	38.0
74-75	35.43184403552837	38.0	36.0	38.0	31.0	38.0
76	34.49517673182629	37.0	34.0	38.0	25.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	2.0
21	8.0
22	25.0
23	66.0
24	156.0
25	301.0
26	592.0
27	1090.0
28	1745.0
29	2924.0
30	4488.0
31	6501.0
32	9218.0
33	13014.0
34	17788.0
35	24865.0
36	37556.0
37	99428.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.38496960431	16.935131593316587	8.881638819118344	26.79825998325507
2	25.16699428488224	19.876870153980562	30.018929052455317	24.937206508681882
3	20.687270212223797	21.325670707291327	29.33684612864475	28.65021295184012
4	14.49073568490408	17.738706272068725	35.19757198500237	32.572986058024824
5	14.096228750318518	32.96567289141276	36.41794983801099	16.520148520257727
6	30.84889519857304	37.78939609042263	17.30233700993775	14.059371701066578
7	29.781860871464456	28.924138182082928	20.35328164245932	20.9407193039933
8	26.29045174911725	35.02557242182666	18.293382112045432	20.390593717010667
9	24.803884095955738	15.835790469950128	19.576098431072765	39.784227003021364
10-11	25.62725237523206	24.43258345163991	26.964571730188197	22.975592442939828
12-13	25.712342106221104	21.921753849514033	26.164637253831312	26.201266790433547
14-15	23.798960722216155	22.70872010483783	22.70052964944851	30.79178952349751
16-17	25.297814058461647	26.65060427359761	23.444951039277782	24.606630628662955
18-19	24.93902660987951	25.473908849332023	24.62551417858833	24.96155036220014
20-21	23.50160168905391	25.387226529795054	24.7397255287394	26.371446252411634
22-23	23.970050234793057	26.15485420989407	24.992947107859198	24.882148447453677
24-25	25.141967893414872	26.14575370390594	24.665556404936115	24.046721997743077
26-27	24.16889629063376	26.471551818281096	25.112391248953443	24.2471606421317
28-29	23.713188453278004	26.06043646026719	25.78878635652142	24.437588729933385
30-31	25.151523424702415	25.940992319172945	25.138782716319025	23.768701539805615
32-33	23.454051545265916	25.76262240180554	24.880328346256054	25.902997706672494
34-35	25.252539041170692	25.797886862509557	25.11989916639365	23.829674929926103
36-37	24.3015361654108	25.61155400240253	24.82117505733319	25.26573477485348
38-39	23.452458956717994	25.385633941247132	26.43856248407411	24.72334461796076
40-41	23.668823486585854	24.305631393105458	27.06376724545885	24.96177787484984
42-43	24.623239052091296	24.391176149393907	26.667667722325362	24.317917076189435
44-45	22.894597939645443	25.658421608241415	27.05443922682101	24.392541225292128
46-47	24.191647555604092	24.12430381129191	26.195806486840667	25.488242146263335
48-49	23.67519384077755	24.09700229332751	26.574387535946997	25.653416329947948
50-51	22.457318626915658	24.17708674602308	27.64301445160351	25.722580175457754
52-53	22.179298168978196	23.90884933202286	29.147555604091586	24.764296894907357
54-55	21.298824214626332	24.389583560845985	28.32623493866259	25.985357285865096
56-57	22.25278475483237	24.43053583779258	27.023952531760763	26.292726875614285
58-59	21.44306723453824	24.332705398420153	28.543282006479558	25.680945360562045
60-61	22.06667940737505	25.19452331549634	26.79825998325507	25.94053729387354
62-63	20.267964398820574	26.32480615922245	26.920661788795456	26.486567653161515
64-65	20.778957810054237	26.613292199046267	26.15735684904081	26.450393141858687
66-67	21.02148629463798	26.481562374868044	25.508945797386335	26.98800553310764
68-69	20.83265079538422	26.951375996505405	25.210904226274984	27.00506898183539
70-71	21.255141785883293	26.46927669178406	25.079856940045868	27.195724582286772
72-73	21.118406683411596	25.297586545811946	24.941756761675947	28.642250009100508
74-75	21.077454406464998	25.747151541625712	24.701958428888645	28.473435623020638
76	21.80026209457246	25.733500782643514	24.2196316115176	28.246605511266427
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	0.5
14	4.5
15	11.5
16	19.0
17	24.0
18	30.5
19	52.0
20	102.0
21	137.0
22	148.0
23	257.5
24	427.0
25	498.0
26	606.5
27	784.5
28	1055.5
29	1257.0
30	1507.0
31	1919.0
32	2646.0
33	3211.0
34	3521.0
35	4305.5
36	5469.0
37	6158.0
38	6747.5
39	8275.5
40	10187.0
41	11852.5
42	12545.0
43	13255.0
44	14130.5
45	14257.0
46	14218.0
47	13877.5
48	12573.0
49	10962.0
50	10315.0
51	9698.0
52	8090.0
53	6544.0
54	5989.0
55	5628.0
56	4758.5
57	4009.0
58	3768.0
59	3394.0
60	2917.5
61	2746.5
62	2678.0
63	2623.0
64	2545.0
65	2572.5
66	2575.0
67	2527.0
68	2521.0
69	2483.5
70	2482.0
71	2512.0
72	2491.5
73	2373.0
74	2194.5
75	2114.0
76	1967.5
77	1628.0
78	1333.5
79	1232.0
80	1079.0
81	766.5
82	526.5
83	446.0
84	362.0
85	228.0
86	126.0
87	74.0
88	58.5
89	35.5
90	17.5
91	5.0
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	219768.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.32980233700994
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.53908294972471	24.41529249026246
2	19.342900983843307	15.601907466055112
3	7.938442097662243	9.604674019875505
4	3.402834190811445	5.489425212041789
5	1.8232692481270871	3.6766044192057077
6	1.1496976261395433	2.7820246805722397
7	0.859734633089629	2.427104947035055
8	0.6227998916869754	2.0093917221797533
9	0.5291542557992599	1.920661788795457
>10	3.6781297951078615	27.125878198827856
>50	0.1049282426211752	2.671908558115831
>100	0.00789782471342179	0.6220195842888865
>500	0.0	0.0
>1k	0.00112826067334597	1.6531069127443485
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGT	3633	1.6531069127443485	No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG	394	0.17927996796621892	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCC	276	0.12558698263623458	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.001820101197626588	0.0
2	0.0	0.0	0.0	0.002275126497033235	0.0
3	0.0	0.0	0.0	0.002275126497033235	0.0
4	0.0	0.0	0.0	0.0040952276946598235	0.0
5	0.0	0.0	0.0	0.00455025299406647	0.0
6	0.0	0.0	0.0	0.00455025299406647	0.0
7	0.0	0.0	0.0	0.005460303592879764	0.0
8	0.0	0.0	0.0	0.005460303592879764	0.0
9	0.0	0.0	0.0	0.006370354191693058	0.0
10	0.0	0.0	0.0	0.007280404790506352	0.0
11	0.0	0.0	0.0	0.007280404790506352	0.0
12	0.0	0.0	0.0	0.007280404790506352	0.0
13	0.0	0.0	0.0	0.007280404790506352	0.0
14	0.0	0.0	0.0	0.0077354300899129995	0.0
15	0.0	0.0	0.0	0.008190455389319647	0.0
16	0.0	0.0	0.0	0.008190455389319647	0.0
17	0.0	0.0	0.0	0.008190455389319647	0.0
18	0.0	0.0	0.0	0.008645480688726294	0.0
19	0.0	0.0	0.0	0.009555531287539587	0.0
20	0.0	0.0	0.0	0.010465581886352882	0.0
21	0.0	0.0	0.0	0.01228568308397947	0.0
22	0.0	0.0	0.0	0.012740708383386116	0.0
23	0.0	0.0	0.0	0.013650758982199411	0.0
24	0.0	0.0	0.0	0.014105784281606058	0.0
25	0.0	0.0	0.0	0.014560809581012704	0.0
26	0.0	0.0	0.0	0.015470860179825999	0.0
27	0.0	0.0	0.0	0.026846492664992172	0.0
28	0.0	0.0	0.0	0.037767099850751704	0.0
29	0.0	0.0	0.0	0.054603035928797644	0.0
30	0.0	0.0	0.0	0.07234902260565687	0.0
31	0.0	0.0	0.0	0.0969203887736158	0.0
32	0.0	0.0	0.0	0.12149175494157476	0.0
33	0.0	0.0	0.0	0.15743875359469986	0.0
34	0.0	0.0	0.0	0.22159732081103709	0.0
35	0.0	0.0	0.0	0.3289832914710058	0.0
36	0.0	0.0	0.0	0.485967019766299	0.0
37	0.0	0.0	0.0	0.7335007826435149	0.0
38	0.0	0.0	0.0	1.052018492228168	0.0
39	0.0	0.0	0.0	1.3891922390884934	0.0
40	0.0	0.0	0.0	1.7614029340031305	0.0
41	0.0	0.0	0.0	2.1595500709839466	0.0
42	0.0	0.0	0.0	2.564977612755269	0.0
43	0.0	0.0	0.0	3.105547668450366	0.0
44	0.0	0.0	0.0	3.691620254086127	0.0
45	0.0	0.0	0.0	4.446052200502348	0.0
46	0.0	0.0	0.0	5.271468093626005	0.0
47	0.0	0.0	0.0	6.173783262349387	0.0
48	0.0	0.0	0.0	7.01603509155109	0.0
49	0.0	0.0	0.0	7.767736886170871	0.0
50	0.0	0.0	0.0	8.518983655491246	0.0
51	0.0	0.0	0.0	9.227003021367988	0.0
52	0.0	0.0	0.0	9.92137162826253	0.0
53	0.0	0.0	0.0	10.589803793090896	0.0
54	0.0	0.0	0.0	11.356976447890503	0.0
55	0.0	0.0	0.0	12.201958428888647	0.0
56	0.0	0.0	0.0	13.047395435186196	0.0
57	0.0	0.0	0.0	13.887827163190273	0.0
58	0.0	0.0	0.0	14.705507626224017	0.0
59	0.0	0.0	0.0	15.539569000036401	0.0
60	0.0	0.0	0.0	16.336318299297442	0.0
61	0.0	0.0	0.0	17.098940701102983	0.0
62	0.0	0.0	0.0	17.795584434494558	0.0
63	0.0	0.0	0.0	18.47903243420334	0.0
64	0.0	0.0	0.0	19.215263368643296	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATCGT	15	0.0022225042	70.0	37
CGCAATA	15	0.0022225042	70.0	28
CACCGAA	15	0.0022225042	70.0	9
CGTTGAA	20	0.006944595	52.5	16
CCGTCTT	520	0.0	52.5	47
AAAAACG	20	0.006944595	52.5	67
CTCGAAA	20	0.006944595	52.5	57
CCGTTTG	20	0.006944595	52.5	63
CGATAGA	20	0.006944595	52.5	8
TCGTATG	550	0.0	52.181816	40
TATGCCG	545	0.0	52.01835	43
CGTCTTC	535	0.0	51.682243	48
CTCGTAT	525	0.0	51.333332	39
ATGCCGT	555	0.0	51.08108	44
CGTATGC	570	0.0	50.96491	41
GCCGTCT	560	0.0	50.0	46
TCTCGTA	490	0.0	49.999996	38
CTTGAAA	550	0.0	48.999996	57
CTGCTTG	565	0.0	47.699116	54
GTATGCC	610	0.0	47.04918	42
>>END_MODULE