FastQCFastQC Report
Wed 25 May 2016
SRR1781132_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781132_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences205688
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGGA350.001253366140.00000412
TAAGGTG350.001253366140.00000420
CTAAGGT501.7056132E-435.0000043
AGTATTC501.7056132E-435.0000048
TAGAGCA450.004300319631.1111094
TAAGGTT604.970242E-429.1666684
GTGAGAT604.970242E-429.16666821
TAATGAA604.970242E-429.16666818
GGTAACG500.007196824528.00000252
TTTAACG500.007196824528.00000234
GGATAAA500.007196824528.0000021
CGCCTAT500.007196824528.0000021
AACGACC500.007196824528.00000255
GAGCAGC500.007196824528.0000026
CACGTAA500.007196824528.00000261
TCTGGTA500.007196824528.00000212
TGCCGCT500.007196824528.00000266
CTCGAAT657.937013E-426.92307712
CGACGAA808.6367494E-526.2570
GTAGCAT700.001223131925.00000236
Produced by FastQC (version 0.11.5)