##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781132_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	205688
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.726201820232586	34.0	31.0	34.0	21.0	34.0
2	30.805311928746452	34.0	32.0	34.0	21.0	34.0
3	30.801412819415813	34.0	32.0	34.0	21.0	34.0
4	30.936116837151413	34.0	32.0	34.0	21.0	34.0
5	31.026778421687215	34.0	32.0	34.0	23.0	34.0
6	33.742299015985374	37.0	34.0	38.0	23.0	38.0
7	33.6522402862588	37.0	34.0	38.0	22.0	38.0
8	33.34884388005134	37.0	34.0	38.0	21.0	38.0
9	33.74475419081327	37.0	34.0	38.0	23.0	38.0
10-11	33.531098070864616	37.0	34.0	38.0	22.0	38.0
12-13	33.492622321185486	37.0	34.0	38.0	22.0	38.0
14-15	33.36801612150441	37.0	34.0	38.0	21.0	38.0
16-17	33.344815934813894	37.0	34.0	38.0	21.5	38.0
18-19	33.387954085799855	37.0	34.0	38.0	21.5	38.0
20-21	33.30672426198903	37.0	34.0	38.0	21.0	38.0
22-23	33.39024882346078	37.0	34.0	38.0	21.5	38.0
24-25	33.37423913888997	37.0	34.0	38.0	21.5	38.0
26-27	33.291395219944775	37.0	34.0	38.0	21.0	38.0
28-29	33.3951202792579	37.0	34.0	38.0	21.5	38.0
30-31	33.45841517249426	37.0	34.0	38.0	22.0	38.0
32-33	33.42705942981603	37.0	34.0	38.0	21.5	38.0
34-35	33.44930428610323	37.0	34.0	38.0	22.0	38.0
36-37	33.28779996888491	37.0	34.0	38.0	21.0	38.0
38-39	33.30655410135739	37.0	34.0	38.0	21.0	38.0
40-41	33.280466045661385	37.0	34.0	38.0	21.0	38.0
42-43	33.333252304461126	37.0	34.0	38.0	21.0	38.0
44-45	33.347224923184626	37.0	34.0	38.0	21.5	38.0
46-47	33.33479104274436	37.0	34.0	38.0	21.5	38.0
48-49	33.24775874139473	37.0	34.0	38.0	21.0	38.0
50-51	33.13927404612812	37.0	33.5	38.0	21.0	38.0
52-53	33.229575862471314	37.0	34.0	38.0	21.0	38.0
54-55	33.1867512932208	37.0	34.0	38.0	21.0	38.0
56-57	33.174098634825555	37.0	34.0	38.0	21.0	38.0
58-59	33.149393741978145	37.0	33.5	38.0	21.0	38.0
60-61	33.120152852864535	37.0	33.5	38.0	21.0	38.0
62-63	33.08177919956439	37.0	33.0	38.0	21.0	38.0
64-65	33.10851386566061	37.0	33.0	38.0	21.0	38.0
66-67	32.99614950799269	37.0	32.5	38.0	21.0	38.0
68-69	32.72094871844736	37.0	31.5	38.0	20.0	38.0
70-71	32.882513807319825	37.0	32.0	38.0	21.0	38.0
72-73	32.849186632180775	37.0	32.0	38.0	21.0	38.0
74-75	32.81745167438062	37.0	31.0	38.0	21.0	38.0
76	31.335060868888803	36.0	27.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	24.0
14	81.0
15	234.0
16	550.0
17	1115.0
18	1570.0
19	1838.0
20	2025.0
21	2357.0
22	2569.0
23	2941.0
24	3449.0
25	4079.0
26	4698.0
27	5471.0
28	6180.0
29	7258.0
30	8242.0
31	9530.0
32	11039.0
33	13179.0
34	15967.0
35	20274.0
36	29143.0
37	51871.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.2671619151336	18.4779860759986	9.584419120220918	25.670432888646882
2	23.67906732526934	19.973941114697986	30.44368169266073	25.90330986737194
3	20.224806503053166	21.172844307883786	28.92439033876551	29.677958850297536
4	14.379059546497608	16.568297615806465	35.599548831239545	33.45309400645638
5	14.542899926101668	33.12978880634748	34.8688304616701	17.458480805880754
6	30.86908327175139	35.537318657384	18.229065380576408	15.364532690288204
7	29.8417992299016	28.457664034848897	21.860293259694295	19.840243475555212
8	26.616526000544518	32.89350861498969	19.36476605344016	21.12519933102563
9	25.111333670413444	16.299929991054412	21.11401734666096	37.47471899187118
10-11	26.336490218194548	23.791373342149274	26.549677180973124	23.322459258683054
12-13	25.942446812648285	22.26065691727276	26.104342499319355	25.692553770759595
14-15	24.128048306172456	23.452510598576485	23.36572867644199	29.05371241880907
16-17	24.43846991560033	26.203035665668388	24.455729065380577	24.902765353350706
18-19	23.70386216016491	25.810207693205246	26.007107852670064	24.478822293959784
20-21	23.82030064952744	25.75915950371436	25.73436466881879	24.686175177939404
22-23	24.05171910855276	25.495653611294777	26.03506281358173	24.41756446657073
24-25	23.669100773987786	25.770341488079033	25.842295126599513	24.71826261133367
26-27	23.203832989770916	25.83305783516783	26.322634281047026	24.640474894014236
28-29	23.696326475049588	25.703978841740888	26.940803547119906	23.65889113608961
30-31	24.043697250204193	25.666543502780907	26.053051223211853	24.23670802380304
32-33	23.440356267745326	25.964810781377622	26.08343705028976	24.511395900587296
34-35	24.422426198903192	25.161652600054452	26.29176228073587	24.124158920306485
36-37	24.023521061024464	25.274201703551007	25.99787056123838	24.704406674186146
38-39	23.480222472871535	25.757944070631243	26.71108669441095	24.050746762086266
40-41	23.93892691843958	25.02357940181245	26.922328964256543	24.115164715491424
42-43	23.622428143596128	25.15873556065497	26.60947648866244	24.609359807086463
44-45	23.08326202792579	25.0031601260161	27.027828555871032	24.88574929018708
46-47	23.21914744661818	24.673291587258372	27.13551592703512	24.97204503908833
48-49	22.516384037960407	24.785840690754927	27.692670452335577	25.005104818949086
50-51	22.674876511998757	24.878213605071757	27.35453696861266	25.09237291431683
52-53	22.448076698689277	24.762990548792345	27.778966201236827	25.009966551281554
54-55	22.038718836295747	25.170160631636264	27.735210610244643	25.055909921823343
56-57	21.690618801291276	25.27882034926685	27.63141262494652	25.399148224495356
58-59	21.890489213005164	24.9538742023701	27.460103312063204	25.695533272561534
60-61	21.920875113947126	24.675782436949255	27.573381950774838	25.829960498328774
62-63	21.358805569600563	24.805773793318036	27.97027536851931	25.865145268562095
64-65	22.091225545486367	24.774658706390262	27.23007662090156	25.90403912722181
66-67	21.963848158375793	24.493893664190423	26.821448018357902	26.72081015907588
68-69	21.75941231379565	24.87456730582241	26.609719575279062	26.756300805102875
70-71	22.394840729648788	24.32737933180351	26.621630819493603	26.656149119054103
72-73	22.230028003578234	23.987058068530978	26.443691416125393	27.339222511765392
74-75	22.45196608455525	24.240111236435766	26.399935825133213	26.90798685387577
76	22.77429893819766	23.66788534090467	25.961164482128275	27.596651238769397
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.5
10	1.5
11	0.5
12	1.0
13	2.5
14	4.5
15	12.5
16	30.5
17	41.0
18	41.5
19	59.0
20	100.0
21	124.0
22	165.0
23	263.0
24	396.5
25	473.0
26	577.5
27	838.0
28	1180.5
29	1367.0
30	1569.0
31	2030.0
32	2804.5
33	3320.0
34	3621.5
35	4420.0
36	5594.5
37	6272.0
38	6898.5
39	8316.5
40	9804.5
41	11050.5
42	11600.0
43	11700.0
44	11984.5
45	12284.5
46	12400.0
47	12186.0
48	11318.5
49	10168.0
50	9671.0
51	9142.0
52	7846.5
53	6599.5
54	6119.0
55	5696.0
56	4899.5
57	4171.0
58	3816.0
59	3523.0
60	3035.5
61	2730.5
62	2620.0
63	2550.0
64	2441.5
65	2407.0
66	2339.0
67	2267.0
68	2312.0
69	2230.0
70	2081.0
71	2059.0
72	2017.0
73	1860.0
74	1665.5
75	1586.0
76	1476.5
77	1266.0
78	1057.5
79	950.0
80	853.5
81	675.5
82	492.0
83	390.0
84	333.5
85	219.0
86	132.0
87	103.0
88	86.5
89	59.0
90	32.5
91	13.5
92	10.0
93	6.5
94	3.0
95	2.5
96	2.0
97	1.5
98	0.5
99	2.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	2.430866166232352E-4
60-61	2.430866166232352E-4
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	205688.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.2729468648246
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.5708811222546	38.66064693959372
2	13.969447592056438	14.883872786051494
3	5.27578842343403	8.431703890549736
4	2.326548882860787	4.95768460060239
5	1.284496828409746	3.4214465643954064
6	0.8177070706803405	2.6136999196407094
7	0.6465828333752653	2.411176104827578
8	0.47051028991346877	2.0052375739130754
9	0.397894052268017	1.907728983387343
>10	2.2262166168203152	20.225101203715393
>50	0.01392628792696271	0.48170143332315385
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	4.861732332464704E-4	0.0
5	0.0	0.0	0.0	9.723464664929408E-4	0.0
6	0.0	0.0	0.0	9.723464664929408E-4	0.0
7	0.0	0.0	0.0	0.001458519699739411	0.0
8	0.0	0.0	0.0	0.001458519699739411	0.0
9	4.861732332464704E-4	0.0	0.0	0.002430866166232352	0.0
10	4.861732332464704E-4	0.0	0.0	0.002430866166232352	0.0
11	4.861732332464704E-4	0.0	0.0	0.002917039399478822	0.0
12	4.861732332464704E-4	0.0	0.0	0.003403212632725293	0.0
13	4.861732332464704E-4	0.0	0.0	0.003403212632725293	0.0
14	4.861732332464704E-4	0.0	0.0	0.003889385865971763	0.0
15	4.861732332464704E-4	4.861732332464704E-4	0.0	0.003889385865971763	0.0
16	4.861732332464704E-4	4.861732332464704E-4	0.0	0.003889385865971763	0.0
17	4.861732332464704E-4	4.861732332464704E-4	0.0	0.004375559099218234	0.0
18	4.861732332464704E-4	4.861732332464704E-4	0.0	0.005834078798957644	0.0
19	4.861732332464704E-4	4.861732332464704E-4	0.0	0.005834078798957644	0.0
20	4.861732332464704E-4	4.861732332464704E-4	0.0	0.005834078798957644	0.0
21	4.861732332464704E-4	4.861732332464704E-4	0.0	0.0063202520322041145	0.0
22	4.861732332464704E-4	4.861732332464704E-4	0.0	0.007778771731943526	0.0
23	4.861732332464704E-4	4.861732332464704E-4	0.0	0.007778771731943526	0.0
24	4.861732332464704E-4	4.861732332464704E-4	0.0	0.010209637898175878	0.0
25	4.861732332464704E-4	4.861732332464704E-4	0.0	0.010209637898175878	0.0
26	4.861732332464704E-4	4.861732332464704E-4	0.0	0.010695811131422348	0.0
27	4.861732332464704E-4	4.861732332464704E-4	0.0	0.019933102563105286	0.0
28	4.861732332464704E-4	4.861732332464704E-4	0.0	0.030142740461281164	0.0
29	4.861732332464704E-4	4.861732332464704E-4	0.0	0.045214110691921744	0.0
30	4.861732332464704E-4	4.861732332464704E-4	0.0	0.057854614756329976	0.0
31	4.861732332464704E-4	4.861732332464704E-4	0.0	0.07098129205398468	0.0
32	4.861732332464704E-4	4.861732332464704E-4	0.0	0.09140056785033643	0.0
33	4.861732332464704E-4	4.861732332464704E-4	0.0	0.11959861537863171	0.0
34	4.861732332464704E-4	4.861732332464704E-4	0.0	0.16335420637081405	0.0
35	4.861732332464704E-4	4.861732332464704E-4	0.0	0.23530784489129167	0.0
36	4.861732332464704E-4	4.861732332464704E-4	0.0	0.35004472793745867	0.0
37	4.861732332464704E-4	4.861732332464704E-4	0.0	0.5474310606355256	0.0
38	4.861732332464704E-4	4.861732332464704E-4	0.0	0.7958655828244721	0.0
39	4.861732332464704E-4	4.861732332464704E-4	0.0	1.073956672241453	0.0
40	4.861732332464704E-4	4.861732332464704E-4	0.0	1.409902376414764	0.0
41	4.861732332464704E-4	4.861732332464704E-4	0.0	1.773559954883124	0.0
42	4.861732332464704E-4	4.861732332464704E-4	0.0	2.2218116759363697	0.0
43	4.861732332464704E-4	4.861732332464704E-4	0.0	2.753199019874762	0.0
44	4.861732332464704E-4	4.861732332464704E-4	0.0	3.334176033604294	0.0
45	4.861732332464704E-4	4.861732332464704E-4	0.0	3.9662012368247055	0.0
46	4.861732332464704E-4	4.861732332464704E-4	0.0	4.665318346233129	0.0
47	4.861732332464704E-4	4.861732332464704E-4	0.0	5.30171910855276	0.0
48	4.861732332464704E-4	4.861732332464704E-4	0.0	5.949788028470304	0.0
49	4.861732332464704E-4	4.861732332464704E-4	0.0	6.5156936719691965	0.0
50	4.861732332464704E-4	4.861732332464704E-4	0.0	7.055832134106025	0.0
51	4.861732332464704E-4	4.861732332464704E-4	0.0	7.648477305433472	0.0
52	4.861732332464704E-4	4.861732332464704E-4	0.0	8.244525689393646	0.0
53	4.861732332464704E-4	4.861732332464704E-4	0.0	8.848352845085762	0.0
54	4.861732332464704E-4	4.861732332464704E-4	0.0	9.488642993271363	0.0
55	4.861732332464704E-4	4.861732332464704E-4	0.0	10.187760102679787	0.0
56	4.861732332464704E-4	4.861732332464704E-4	0.0	10.871319668624324	0.0
57	4.861732332464704E-4	4.861732332464704E-4	0.0	11.50140017891175	0.0
58	4.861732332464704E-4	4.861732332464704E-4	0.0	12.13780094123138	0.0
59	4.861732332464704E-4	4.861732332464704E-4	0.0	12.684259655400412	0.0
60	4.861732332464704E-4	4.861732332464704E-4	0.0	13.271070747928903	0.0
61	4.861732332464704E-4	4.861732332464704E-4	0.0	13.838921084360779	0.0
62	4.861732332464704E-4	4.861732332464704E-4	0.0	14.398506475827467	0.0
63	4.861732332464704E-4	4.861732332464704E-4	0.0	14.969273851658823	0.0
64	4.861732332464704E-4	4.861732332464704E-4	0.0	15.599354361946249	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGGGA	35	0.0012533661	40.000004	12
TAAGGTG	35	0.0012533661	40.000004	20
CTAAGGT	50	1.7056132E-4	35.000004	3
AGTATTC	50	1.7056132E-4	35.000004	8
TAGAGCA	45	0.0043003196	31.111109	4
TAAGGTT	60	4.970242E-4	29.166668	4
GTGAGAT	60	4.970242E-4	29.166668	21
TAATGAA	60	4.970242E-4	29.166668	18
GGTAACG	50	0.0071968245	28.000002	52
TTTAACG	50	0.0071968245	28.000002	34
GGATAAA	50	0.0071968245	28.000002	1
CGCCTAT	50	0.0071968245	28.000002	1
AACGACC	50	0.0071968245	28.000002	55
GAGCAGC	50	0.0071968245	28.000002	6
CACGTAA	50	0.0071968245	28.000002	61
TCTGGTA	50	0.0071968245	28.000002	12
TGCCGCT	50	0.0071968245	28.000002	66
CTCGAAT	65	7.937013E-4	26.923077	12
CGACGAA	80	8.6367494E-5	26.25	70
GTAGCAT	70	0.0012231319	25.000002	36
>>END_MODULE