FastQCFastQC Report
Wed 25 May 2016
SRR1781132_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781132_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186964
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT26301.4066879185297705No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG2830.15136603838172055No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCC1960.10483301598168632No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3850.060.9090943
CGTATGC3800.060.7894741
CTCGTAT3800.060.7894739
CGTCTTC3800.059.8684248
CCGTCTT3800.059.8684247
TCTCGTA3400.059.70588738
TCGTATG3900.059.2307740
ATCTCGT3450.058.84057637
TACCTCG308.352788E-658.33333636
GCCGTCT4000.057.75000446
GTATGCC4100.057.1951242
GTATCTC3600.056.38888535
ATGCCGT4050.056.1728444
TGCCGTC4050.056.1728445
TAGTATC3900.053.84615333
ATTAGAG200.00694298152.53
CGCATCT200.00694298152.515
AAAAACG200.00694298152.566
GTCTTCT4350.052.2988549
TATCTCG4000.051.62500436