##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781132_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	186964
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.28255706981023	34.0	34.0	34.0	32.0	34.0
2	33.28717828031065	34.0	34.0	34.0	33.0	34.0
3	33.335326586936525	34.0	34.0	34.0	33.0	34.0
4	33.392380351297575	34.0	34.0	34.0	33.0	34.0
5	33.38113754519587	34.0	34.0	34.0	33.0	34.0
6	36.80207419610193	38.0	37.0	38.0	34.0	38.0
7	36.71010461907105	38.0	37.0	38.0	34.0	38.0
8	36.492886331058386	38.0	37.0	38.0	34.0	38.0
9	36.47070559038104	38.0	37.0	38.0	34.0	38.0
10-11	36.36440972593654	38.0	37.0	38.0	34.0	38.0
12-13	36.31216704820179	38.0	37.0	38.0	34.0	38.0
14-15	36.31680697888363	38.0	37.0	38.0	34.0	38.0
16-17	36.24883132581674	38.0	37.0	38.0	34.0	38.0
18-19	36.25768864594254	38.0	37.0	38.0	34.0	38.0
20-21	36.16651601377805	38.0	37.0	38.0	33.0	38.0
22-23	36.25151900900708	38.0	37.0	38.0	34.0	38.0
24-25	36.229046768361826	38.0	37.0	38.0	34.0	38.0
26-27	36.02146937378319	38.0	37.0	38.0	32.5	38.0
28-29	36.000783573308226	38.0	37.0	38.0	32.0	38.0
30-31	35.99660362422712	38.0	37.0	38.0	32.0	38.0
32-33	35.949765730301024	38.0	37.0	38.0	32.0	38.0
34-35	35.985778010740034	38.0	37.0	38.0	32.0	38.0
36-37	35.94018634603453	38.0	36.5	38.0	32.0	38.0
38-39	35.85144198883207	38.0	36.0	38.0	31.0	38.0
40-41	35.90056909351533	38.0	36.5	38.0	32.0	38.0
42-43	35.92570762285788	38.0	36.5	38.0	32.0	38.0
44-45	35.879789692133244	38.0	36.0	38.0	31.5	38.0
46-47	35.91176376200766	38.0	36.5	38.0	32.0	38.0
48-49	35.88761205365739	38.0	36.5	38.0	32.0	38.0
50-51	35.78664074367258	38.0	36.0	38.0	31.0	38.0
52-53	35.81041804839434	38.0	36.0	38.0	31.0	38.0
54-55	35.811653580368414	38.0	36.0	38.0	31.0	38.0
56-57	35.777513318071925	38.0	36.0	38.0	31.0	38.0
58-59	35.759266489805526	38.0	36.0	38.0	31.0	38.0
60-61	35.75447412336065	38.0	36.0	38.0	31.0	38.0
62-63	35.73891765259622	38.0	36.0	38.0	31.0	38.0
64-65	35.68608662630239	38.0	36.0	38.0	31.0	38.0
66-67	35.648734515735654	38.0	36.0	38.0	31.0	38.0
68-69	35.556609828630116	38.0	36.0	38.0	31.0	38.0
70-71	35.45184367043923	38.0	36.0	38.0	30.5	38.0
72-73	35.34687159025267	38.0	36.0	38.0	29.5	38.0
74-75	35.343346847521445	38.0	36.0	38.0	30.0	38.0
76	34.386470122590445	37.0	34.0	38.0	25.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	3.0
21	9.0
22	28.0
23	52.0
24	139.0
25	275.0
26	494.0
27	977.0
28	1613.0
29	2517.0
30	4020.0
31	5933.0
32	8430.0
33	11500.0
34	15649.0
35	21408.0
36	32839.0
37	81077.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	44.495196936308595	18.495004385871077	9.973042938747566	27.036755739072767
2	23.611497400569093	20.225818874221776	30.94659934532851	25.21608437988062
3	20.430671145247214	21.860358143813784	28.721572067349864	28.98739864358914
4	14.818360753941937	17.576645771378445	34.76712094306925	32.837872531610365
5	14.687319483964828	33.26148349414861	35.209986949359234	16.841210072527332
6	30.887764489420423	36.96005648146167	17.4306283562611	14.721550672856806
7	29.59339765944246	28.58625189876126	20.75158854110952	21.068761900686763
8	26.20023106052502	34.822746625018716	18.34631265912154	20.630709655334716
9	24.505787210372052	16.179585374724546	20.11724182195503	39.19738559294837
10-11	25.719389829058	24.88714404912176	26.513392952653987	22.880073169166256
12-13	25.441796281637107	22.390674140476243	26.010087503476605	26.15744207441005
14-15	23.467619434757495	23.17291029289061	22.68832502513853	30.671145247213367
16-17	24.84488992533322	27.083021330309577	23.81126847949338	24.260820264863824
18-19	24.663571596671016	25.6257889219315	24.925654136625234	24.784985344772256
20-21	23.42857448492758	25.703878821591324	24.79701974711709	26.070526946364005
22-23	23.659100147622002	26.334214073297534	25.167144477011615	24.83954130206885
24-25	24.45256840889155	26.51900900708158	24.99839541302069	24.030027171006182
26-27	23.731573992854237	26.782963565178324	25.281070152542735	24.204392289424703
28-29	24.566226653259452	25.326800881453117	25.858454033931665	24.24851843135577
30-31	23.724353351447338	26.121606298538758	25.45971416957275	24.694326180441152
32-33	22.85413234633405	25.914347147044353	27.025256199054365	24.20626430756723
34-35	24.520228493185854	26.105025566419204	25.731691662566053	23.643054277828888
36-37	23.922252412229092	25.91621916518688	26.06170171797779	24.099826704606233
38-39	22.95762820649965	25.9309278791639	26.464453049784986	24.646990864551466
40-41	23.202060289681437	24.822960569949295	27.14560022250273	24.829378917866542
42-43	23.952204702509576	24.7914036926895	26.803288333582937	24.453103271217987
44-45	22.62521127061894	25.81192101153163	27.057615369803813	24.505252348045616
46-47	23.75778224684966	24.22578678248219	26.807032369867994	25.209398600800153
48-49	23.151783230996344	24.421813825121415	26.986746111550886	25.43965683233136
50-51	22.26792323655891	24.614096831475578	27.49700477097195	25.62097516099356
52-53	21.79644209580454	24.539216105774372	28.85715966710169	24.8071821313194
54-55	21.44022378639738	24.905864230547056	27.968485911726322	25.685426071329243
56-57	22.05371087482082	24.86173808861599	27.040232344194603	26.044318692368584
58-59	21.34929719090306	24.65340921246871	28.53677713356582	25.46051646306241
60-61	22.09382554930361	25.221967865471427	26.955189234291094	25.72901735093387
62-63	20.564921589182944	26.33448150446075	26.75140668791853	26.349190218437773
64-65	21.08026144070516	26.711292013435738	26.016238420230632	26.192208125628465
66-67	21.064750433238487	26.487986992148222	25.669112770372905	26.778149804240385
68-69	20.989067414047625	26.83136860572089	25.241222909223165	26.93834107100832
70-71	21.43300314499048	26.325121413748104	25.306208681885284	26.935666759376137
72-73	21.27200958473289	25.57952333069468	25.21956098500246	27.92890609956997
74-75	21.504674696733062	25.58834855908089	25.37734537130143	27.529631372884623
76	21.890310434094264	25.572837551614214	25.22892107571511	27.30793093857641
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	0.5
10	0.0
11	0.0
12	0.0
13	0.0
14	4.0
15	9.0
16	20.0
17	30.0
18	42.0
19	75.0
20	107.5
21	119.0
22	150.0
23	245.0
24	416.5
25	524.0
26	618.0
27	805.0
28	1091.5
29	1285.0
30	1443.0
31	1819.0
32	2487.0
33	2937.0
34	3299.5
35	3941.5
36	4867.5
37	5514.0
38	6013.5
39	7446.5
40	9290.5
41	10680.5
42	11160.0
43	11329.5
44	11233.0
45	11081.5
46	11196.0
47	10991.0
48	10188.5
49	9234.0
50	8877.0
51	8261.5
52	6849.0
53	5763.0
54	5474.0
55	5054.5
56	4278.5
57	3607.0
58	3292.0
59	2969.5
60	2519.0
61	2365.5
62	2340.0
63	2291.5
64	2195.5
65	2151.5
66	2164.0
67	2173.0
68	2121.5
69	2007.5
70	1951.5
71	1958.0
72	1892.0
73	1769.0
74	1597.0
75	1482.0
76	1413.0
77	1235.0
78	1001.0
79	876.0
80	791.5
81	601.5
82	439.0
83	382.0
84	308.5
85	191.0
86	112.0
87	77.0
88	58.5
89	34.5
90	22.5
91	9.5
92	3.0
93	3.5
94	3.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	186964.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.27983996919193
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.83473309944836	28.45146659249909
2	18.912789255466176	17.12736141717122
3	7.466600517381907	10.14259429622815
4	3.197609175850786	5.791489270661732
5	1.7151564548708316	3.883100489933891
6	1.005232880919475	2.7310070387882157
7	0.807966263864772	2.5609208189811943
8	0.6260557307724111	2.2678162640936224
9	0.5457315992770828	2.223957553325774
>10	2.8467817191726614	22.047025095740356
>50	0.03661835406404668	1.0338888770030594
>100	0.003543711683617421	0.3326843670439229
>500	0.0	0.0
>1k	0.0011812372278724736	1.4066879185297705
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT	2630	1.4066879185297705	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG	283	0.15136603838172055	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCC	196	0.10483301598168632	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	5.34862326437175E-4	0.0
5	0.0	0.0	0.0	0.00106972465287435	0.0
6	0.0	0.0	0.0	0.00106972465287435	0.0
7	0.0	0.0	0.0	0.0016045869793115252	0.0
8	0.0	0.0	0.0	0.0016045869793115252	0.0
9	0.0	0.0	0.0	0.0026743116321858752	0.0
10	0.0	0.0	0.0	0.0026743116321858752	0.0
11	0.0	0.0	0.0	0.0032091739586230504	0.0
12	0.0	0.0	0.0	0.0042788986114974	0.0
13	0.0	0.0	0.0	0.0042788986114974	0.0
14	0.0	0.0	0.0	0.004813760937934576	0.0
15	0.0	0.0	0.0	0.004813760937934576	0.0
16	0.0	0.0	0.0	0.004813760937934576	0.0
17	0.0	0.0016045869793115252	0.0	0.0053486232643717505	0.0
18	0.0	0.0016045869793115252	0.0	0.006953210243683276	0.0
19	0.0	0.0016045869793115252	0.0	0.007488072570120451	0.0
20	0.0	0.0016045869793115252	0.0	0.007488072570120451	0.0
21	0.0	0.0016045869793115252	0.0	0.008022934896557626	0.0
22	0.0	0.0016045869793115252	0.0	0.011232108855180677	0.0
23	0.0	0.0016045869793115252	0.0	0.011766971181617852	0.0
24	0.0	0.0016045869793115252	0.0	0.012301833508055027	0.0
25	0.0	0.0016045869793115252	0.0	0.012301833508055027	0.0
26	0.0	0.0016045869793115252	0.0	0.012836695834492201	0.0
27	0.0	0.0016045869793115252	0.0	0.021929355383924178	0.0
28	0.0	0.0016045869793115252	0.0	0.033161464239104856	0.0
29	0.0	0.0016045869793115252	0.0	0.04974219635865728	0.0
30	0.0	0.0016045869793115252	0.0	0.06364861684602384	0.0
31	0.0	0.0016045869793115252	0.0	0.07969448663913908	0.0
32	0.0	0.0016045869793115252	0.0	0.10269356667593761	0.0
33	0.0	0.0016045869793115252	0.0	0.13425044393573093	0.0
34	0.0	0.0016045869793115252	0.0	0.18720181425301127	0.0
35	0.0	0.0016045869793115252	0.0	0.2727797864829593	0.0
36	0.0	0.0016045869793115252	0.0	0.4113091290301876	0.0
37	0.0	0.0016045869793115252	0.0	0.6332769945016152	0.0
38	0.0	0.0016045869793115252	0.0	0.9135448535546951	0.0
39	0.0	0.0016045869793115252	0.0	1.2366016987227488	0.0
40	0.0	0.0016045869793115252	0.0	1.6051218416379625	0.0
41	0.0	0.0016045869793115252	0.0	2.0084080357715925	0.0
42	0.0	0.0016045869793115252	0.0	2.49352816585011	0.0
43	0.0	0.0016045869793115252	0.0	3.0717143407286964	0.0
44	0.0	0.0016045869793115252	0.0	3.7172931687383666	0.0
45	0.0	0.0016045869793115252	0.0	4.407265569842322	0.0
46	0.0	0.0016045869793115252	0.0	5.167304935709549	0.0
47	0.0	0.0016045869793115252	0.0	5.870114032647996	0.0
48	0.0	0.0016045869793115252	0.0	6.567574506322073	0.0
49	0.0	0.0016045869793115252	0.0	7.172503797522518	0.0
50	0.0	0.0016045869793115252	0.0	7.744271624483858	0.0
51	0.0	0.0016045869793115252	0.0	8.381827517596971	0.0
52	0.0	0.0016045869793115252	0.0	9.032754968871012	0.0
53	0.0	0.0016045869793115252	0.0	9.67886865920712	0.0
54	0.0	0.0016045869793115252	0.0	10.377933719860508	0.0
55	0.0	0.0016045869793115252	0.0	11.141182259686357	0.0
56	0.0	0.0016045869793115252	0.0	11.881966581801844	0.0
57	0.0	0.0016045869793115252	0.0	12.591194026657538	0.0
58	0.0	0.0016045869793115252	0.0	13.272073768212062	0.0
59	0.0	0.0016045869793115252	0.0	13.887165443614814	0.0
60	0.0	0.0016045869793115252	0.0	14.510814916240559	0.0
61	0.0	0.0016045869793115252	0.0	15.121627693031813	0.0
62	0.0	0.0016045869793115252	0.0	15.725487259579385	0.0
63	0.0	0.0016045869793115252	0.0	16.329346826126955	0.0
64	0.0	0.0016045869793115252	0.0	17.016644915598725	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGCCG	385	0.0	60.90909	43
CGTATGC	380	0.0	60.78947	41
CTCGTAT	380	0.0	60.78947	39
CGTCTTC	380	0.0	59.86842	48
CCGTCTT	380	0.0	59.86842	47
TCTCGTA	340	0.0	59.705887	38
TCGTATG	390	0.0	59.23077	40
ATCTCGT	345	0.0	58.840576	37
TACCTCG	30	8.352788E-6	58.333336	36
GCCGTCT	400	0.0	57.750004	46
GTATGCC	410	0.0	57.19512	42
GTATCTC	360	0.0	56.388885	35
ATGCCGT	405	0.0	56.17284	44
TGCCGTC	405	0.0	56.17284	45
TAGTATC	390	0.0	53.846153	33
ATTAGAG	20	0.006942981	52.5	3
CGCATCT	20	0.006942981	52.5	15
AAAAACG	20	0.006942981	52.5	66
GTCTTCT	435	0.0	52.29885	49
TATCTCG	400	0.0	51.625004	36
>>END_MODULE