FastQCFastQC Report
Wed 25 May 2016
SRR1781131_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781131_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences293372
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGA200.00694690252.50000433
CAATACG404.5864E-543.75000469
AGACTCG400.002416506635.00000431
GAAGTAC400.002416506635.00000437
CATCGTT806.224036E-835.00000432
GAACCGT400.002416506635.00000469
GCCGTTA400.002416506635.00000467
CGAAGTA400.002416506635.00000436
AGACCGC652.0987156E-532.30769317
TTAAGGT552.988062E-431.8181843
CACGGTT450.00430328231.11111349
ACTCTGA450.00430328231.11111342
ACGGCGT450.00430328231.11111341
TTCGGTC901.7511411E-731.11111328
TCGAAGT450.00430328231.11111335
GTCGGAA450.00430328231.11111313
ACGGTTA450.00430328231.11111350
TCGTTGT802.4603414E-630.62500234
CGCAACC703.4820787E-530.00000221
CGAATAT604.975356E-429.16666625