##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781131_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	293372
Sequences flagged as poor quality	0
Sequence length	76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.726623535988438	34.0	33.0	34.0	24.0	34.0
2	31.893888987360757	34.0	34.0	34.0	24.0	34.0
3	31.82452313104182	34.0	34.0	34.0	23.0	34.0
4	31.98578255593581	34.0	34.0	34.0	27.0	34.0
5	32.0083750323821	34.0	34.0	34.0	27.0	34.0
6	34.969724445414016	38.0	36.0	38.0	27.0	38.0
7	34.948423844129636	38.0	35.0	38.0	27.0	38.0
8	34.71933586027296	38.0	35.0	38.0	27.0	38.0
9	34.93487108517514	38.0	36.0	38.0	27.0	38.0
10-11	34.827727254134686	38.0	35.0	38.0	27.0	38.0
12-13	34.79642569843067	38.0	35.0	38.0	27.0	38.0
14-15	34.67195062923524	38.0	35.0	38.0	26.0	38.0
16-17	34.658201873389416	38.0	35.0	38.0	26.5	38.0
18-19	34.70708520240514	38.0	35.0	38.0	26.5	38.0
20-21	34.63831926700571	38.0	34.5	38.0	26.5	38.0
22-23	34.703766889819065	38.0	35.0	38.0	26.5	38.0
24-25	34.67625915220266	38.0	35.0	38.0	26.5	38.0
26-27	34.61853210258647	38.0	35.0	38.0	26.0	38.0
28-29	34.72077260270237	38.0	35.0	38.0	26.5	38.0
30-31	34.77557844647751	38.0	35.0	38.0	27.0	38.0
32-33	34.71273843447909	38.0	35.0	38.0	26.5	38.0
34-35	34.738340741447715	38.0	35.0	38.0	26.5	38.0
36-37	34.60320514568534	38.0	35.0	38.0	26.0	38.0
38-39	34.61571486031387	38.0	35.0	38.0	26.0	38.0
40-41	34.59474148862195	38.0	34.5	38.0	26.0	38.0
42-43	34.67507464925078	38.0	35.0	38.0	26.5	38.0
44-45	34.68241515891087	38.0	35.0	38.0	26.5	38.0
46-47	34.68540965054606	38.0	35.0	38.0	26.5	38.0
48-49	34.659585781874206	38.0	35.0	38.0	26.5	38.0
50-51	34.53019545150866	37.5	34.5	38.0	25.5	38.0
52-53	34.611419971912795	38.0	35.0	38.0	26.0	38.0
54-55	34.5936643578801	38.0	35.0	38.0	26.0	38.0
56-57	34.563003626794654	37.5	34.5	38.0	25.5	38.0
58-59	34.55315606124647	37.5	34.5	38.0	25.5	38.0
60-61	34.53489937690031	37.0	34.5	38.0	25.5	38.0
62-63	34.49876095878271	37.0	34.0	38.0	25.0	38.0
64-65	34.52524780824346	37.0	34.0	38.0	25.0	38.0
66-67	34.424418485745065	37.0	34.0	38.0	25.0	38.0
68-69	34.20309368310541	37.0	34.0	38.0	24.5	38.0
70-71	34.36992282835445	37.0	34.0	38.0	25.0	38.0
72-73	34.33525694340291	37.0	34.0	38.0	24.5	38.0
74-75	34.32824195901449	37.0	34.0	38.0	24.5	38.0
76	32.9430518249867	37.0	32.0	38.0	22.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	5.0
13	18.0
14	91.0
15	288.0
16	585.0
17	1051.0
18	1415.0
19	1427.0
20	1511.0
21	1569.0
22	1681.0
23	2076.0
24	2579.0
25	3085.0
26	3769.0
27	4697.0
28	5836.0
29	7204.0
30	8929.0
31	10847.0
32	13708.0
33	17349.0
34	22171.0
35	30322.0
36	46867.0
37	104292.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.184557490149025	17.91343413822723	9.356721159483522	24.545287212140217
2	24.216694163042142	20.547973221711683	30.964441050952374	24.270891564293798
3	19.46198001172573	22.501465715882908	30.05501547523281	27.981538797158557
4	13.512877847920047	16.700980325320753	38.863967931499936	30.922173895259263
5	12.785132868849105	34.84620209154248	36.99569147703257	15.372973562575842
6	28.730417354076053	38.90214471728727	18.78843243390644	13.57900549473024
7	28.988451522299336	29.96707252225843	22.796994941575882	18.247481013866356
8	25.667071158801797	34.95630121483986	19.52742593021829	19.849201696140053
9	24.949211240336503	16.132759772575433	21.468647314672157	37.44938167241591
10-11	24.9933531489031	24.65692022415227	28.39807479923101	21.95165182771362
12-13	25.245933490585333	23.317324080007634	26.85907312217935	24.577669307227684
14-15	23.522013007376298	24.27276631716728	24.166416699616867	28.03880397583955
16-17	23.641145030882292	27.162783087683895	25.525612532893394	23.670459348540422
18-19	22.899765485458733	27.09835976166778	26.861800035449875	23.140074717423612
20-21	23.08655904448959	27.00666730294643	26.630694135773012	23.27607951679097
22-23	23.312551981784218	26.438787614359928	27.203175490503522	23.045484913352333
24-25	22.881017956723888	26.938324039103932	27.15732926114285	23.023328743029328
26-27	22.301548886737656	27.21016320575924	27.145569447663714	23.342718459839386
28-29	23.048723122861077	26.75442782542301	27.993639474796506	22.203209576919406
30-31	23.090478982315968	26.593369510382725	27.45149503020056	22.864656477100745
32-33	22.664228351717274	27.271177890187204	26.89827250044312	23.1663212576524
34-35	23.71085175136005	26.085141049588916	27.36525639802026	22.838750801030773
36-37	23.835608033486494	26.12161351458217	26.593880806620945	23.44889764531039
38-39	23.011057633311975	26.941562248612684	27.333385599171017	22.713994518904325
40-41	23.1530275554586	25.940955510409992	28.0399970003954	22.866019933736005
42-43	22.941862209072443	26.238189056897042	27.48745619895559	23.332492535074923
44-45	22.31859209467843	26.479691313417774	27.703904939803394	23.497811652100406
46-47	22.60406582768635	25.670479800389952	27.949838430388724	23.77561594153498
48-49	21.9843747869599	25.744788187011714	28.580437124197267	23.69039990183112
50-51	22.107597180371677	25.58032123038327	28.275363702057454	24.0367178871876
52-53	21.924041830849568	25.577083020874518	28.683548532238934	23.815326616036977
54-55	20.999618232142126	26.389021447172873	28.843584254802778	23.767776065882224
56-57	21.008651132350735	25.99344859086757	28.65917674488363	24.33872353189807
58-59	21.412916024705833	26.002651923155582	28.162708097568956	24.421723954569625
60-61	20.96740656913407	25.633325607079065	28.627817242272606	24.771450581514255
62-63	20.318912506987715	25.494764326520595	29.314488090206293	24.871835076285397
64-65	21.29088665584991	25.74069781710593	28.294111230792307	24.674304296251858
66-67	21.31508801112581	25.534986297260815	27.919331088174744	25.23059460343864
68-69	20.90247194687973	25.51385271941426	27.72384549309409	25.85982984061192
70-71	21.656633898258864	25.253773366238086	27.780599375536863	25.308993359966188
72-73	21.57721254925487	24.519211103990838	27.384174358834517	26.51940198791977
74-75	21.65680433033827	24.884617482240976	27.28941412268383	26.169164064736922
76	22.06345527180508	24.54290116302851	27.157670125301664	26.235973439864747
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	0.5
11	0.0
12	0.0
13	1.5
14	3.0
15	8.5
16	24.5
17	35.0
18	39.5
19	59.5
20	94.0
21	113.0
22	148.5
23	318.0
24	529.0
25	606.0
26	851.0
27	1139.0
28	1527.0
29	1872.0
30	2152.5
31	2677.5
32	3779.0
33	4636.0
34	5284.0
35	6648.0
36	8448.5
37	9533.0
38	10588.0
39	13032.0
40	15934.5
41	18311.0
42	19174.0
43	19483.5
44	19898.5
45	20332.5
46	20661.0
47	20214.0
48	18718.5
49	16780.5
50	15891.0
51	14799.0
52	12072.5
53	9685.0
54	8932.0
55	8182.0
56	6582.5
57	5096.0
58	4459.0
59	3884.5
60	2922.5
61	2400.0
62	2265.0
63	2074.0
64	1883.0
65	1791.0
66	1656.0
67	1613.0
68	1604.5
69	1587.0
70	1498.5
71	1419.0
72	1378.0
73	1328.0
74	1259.0
75	1199.0
76	1069.0
77	894.5
78	748.5
79	647.0
80	576.0
81	444.5
82	320.5
83	257.0
84	212.5
85	137.0
86	96.0
87	86.0
88	66.5
89	32.5
90	21.0
91	17.5
92	11.0
93	9.0
94	6.5
95	3.0
96	0.0
97	0.5
98	0.5
99	3.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	293372.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.706598768875075
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.55139755775143	23.763240501025226
2	14.235776910418831	10.166223486070827
3	6.082742502979131	6.515821379047762
4	2.808441683168914	4.011196013867863
5	1.7240902016504696	3.078069853584111
6	1.1771656366311747	2.521954864301805
7	0.9012840100992925	2.252725056779273
8	0.7030992579403202	2.0084226478375053
9	0.5955427395833068	1.9138325086816017
>10	4.869905943671296	34.692922989567684
>50	0.31669326943652104	7.540826595408088
>100	0.033860286669313566	1.5347641038282238
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.002386049111708002	0.0
2	0.0	0.0	0.0	0.002386049111708002	0.0
3	0.0	0.0	0.0	0.002726913270523431	0.0
4	0.0	0.0	0.0	0.0034086415881542887	0.0
5	0.0	0.0	0.0	0.0037495057469697176	0.0
6	0.0	0.0	0.0	0.0037495057469697176	0.0
7	0.0	0.0	0.0	0.0037495057469697176	0.0
8	0.0	0.0	0.0	0.0037495057469697176	0.0
9	0.0	0.0	0.0	0.0040903699057851465	0.0
10	0.0	0.0	0.0	0.0040903699057851465	0.0
11	0.0	0.0	0.0	0.0040903699057851465	0.0
12	0.0	0.0	0.0	0.0040903699057851465	0.0
13	0.0	0.0	0.0	0.0040903699057851465	0.0
14	0.0	0.0	0.0	0.0040903699057851465	0.0
15	0.0	0.0	0.0	0.0040903699057851465	0.0
16	0.0	0.0	0.0	0.004431234064600576	0.0
17	0.0	0.0	0.0	0.004431234064600576	0.0
18	0.0	0.0	0.0	0.004431234064600576	0.0
19	0.0	0.0	0.0	0.004772098223416004	0.0
20	0.0	0.0	0.0	0.004772098223416004	0.0
21	0.0	0.0	0.0	0.0051129623822314335	0.0
22	0.0	0.0	0.0	0.00613555485867772	0.0
23	0.0	0.0	0.0	0.0068172831763085775	0.0
24	0.0	0.0	0.0	0.007839875652754864	0.0
25	0.0	0.0	0.0	0.007839875652754864	0.0
26	0.0	0.0	0.0	0.00920333228801658	0.0
27	0.0	0.0	0.0	0.012952838034986298	0.0
28	0.0	0.0	0.0	0.02113357784655659	0.0
29	0.0	0.0	0.0	0.03681332915206632	0.0
30	0.0	0.0	0.0	0.044312340646005755	0.0
31	0.0	0.0	0.0	0.0613555485867772	0.0
32	0.0	0.0	0.0	0.07873962068636407	0.0
33	0.0	0.0	0.0	0.09748714942121266	0.0
34	0.0	0.0	0.0	0.141458625908403	0.0
35	0.0	0.0	0.0	0.20963145767148875	0.0
36	3.408641588154289E-4	0.0	0.0	0.31598107522190255	0.0
37	3.408641588154289E-4	0.0	0.0	0.49186698117066385	0.0
38	3.408641588154289E-4	0.0	0.0	0.7195642392593704	0.0
39	3.408641588154289E-4	0.0	0.0	0.9912329738352672	0.0
40	3.408641588154289E-4	0.0	0.0	1.2768771389225966	0.0
41	3.408641588154289E-4	0.0	0.0	1.5805871044271438	0.0
42	3.408641588154289E-4	0.0	0.0	1.9422439769303137	0.0
43	3.408641588154289E-4	0.0	0.0	2.3754823227847237	0.0
44	3.408641588154289E-4	0.0	0.0	2.941998554735967	0.0
45	3.408641588154289E-4	0.0	0.0	3.6373614387194415	0.0
46	3.408641588154289E-4	0.0	0.0	4.414531720818619	0.0
47	3.408641588154289E-4	0.0	0.0	5.214880765717246	0.0
48	3.408641588154289E-4	0.0	0.0	5.941603152311741	0.0
49	3.408641588154289E-4	0.0	0.0	6.6315122097541686	0.0
50	3.408641588154289E-4	0.0	0.0	7.281540160615192	0.0
51	3.408641588154289E-4	0.0	0.0	7.914524903535443	0.0
52	3.408641588154289E-4	0.0	0.0	8.521603970385723	0.0
53	3.408641588154289E-4	0.0	0.0	9.110958100977598	0.0
54	3.408641588154289E-4	0.0	0.0	9.793368146926086	0.0
55	3.408641588154289E-4	0.0	0.0	10.525885224220444	0.0
56	3.408641588154289E-4	0.0	0.0	11.284307977584772	0.0
57	3.408641588154289E-4	0.0	0.0	12.082611837530507	0.0
58	3.408641588154289E-4	0.0	0.0	12.84819273822996	0.0
59	3.408641588154289E-4	0.0	0.0	13.530261920019633	0.0
60	3.408641588154289E-4	0.0	0.0	14.256302578296497	0.0
61	3.408641588154289E-4	0.0	0.0	14.915533861445537	0.0
62	3.408641588154289E-4	0.0	0.0	15.551927245953943	0.0
63	3.408641588154289E-4	0.0	0.0	16.178776434015518	0.0
64	3.408641588154289E-4	0.0	0.0	16.829145249035353	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAAAGA	20	0.006946902	52.500004	33
CAATACG	40	4.5864E-5	43.750004	69
AGACTCG	40	0.0024165066	35.000004	31
GAAGTAC	40	0.0024165066	35.000004	37
CATCGTT	80	6.224036E-8	35.000004	32
GAACCGT	40	0.0024165066	35.000004	69
GCCGTTA	40	0.0024165066	35.000004	67
CGAAGTA	40	0.0024165066	35.000004	36
AGACCGC	65	2.0987156E-5	32.307693	17
TTAAGGT	55	2.988062E-4	31.81818	43
CACGGTT	45	0.004303282	31.111113	49
ACTCTGA	45	0.004303282	31.111113	42
ACGGCGT	45	0.004303282	31.111113	41
TTCGGTC	90	1.7511411E-7	31.111113	28
TCGAAGT	45	0.004303282	31.111113	35
GTCGGAA	45	0.004303282	31.111113	13
ACGGTTA	45	0.004303282	31.111113	50
TCGTTGT	80	2.4603414E-6	30.625002	34
CGCAACC	70	3.4820787E-5	30.000002	21
CGAATAT	60	4.975356E-4	29.166666	25
>>END_MODULE