FastQCFastQC Report
Wed 25 May 2016
SRR1781131_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781131_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences265970
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT29371.1042598789337144No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG3500.1315937887731699No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCC2870.10790690679399932No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACTCG252.3876855E-455.99999636
AATATCG252.3876855E-455.99999636
ATATCGT252.3876855E-455.99999637
AGCCACG200.006946192652.544
CTCTCGG200.006946192652.532
TCGTATG4250.051.05882340
CTCGTAT3950.050.50633239
TATGCCG4300.050.4651243
ATCGTAT352.0831096E-550.039
CGTATGC4350.049.8850641
CCGTCTT4350.049.08046347
CGTCTTC4350.048.27586448
ATCTCGT3700.048.24324437
GCCGTCT4450.047.97752846
AATCTCG3800.047.89473336
TCTCGTA3750.047.59999538
TCTTCTG4150.047.22891650
ATGCCGT4700.046.91489444
TAGGTTC305.87121E-446.66666845
AACTCGT305.87121E-446.66666837
Produced by FastQC (version 0.11.5)